| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 596,033 | C→A | 9.0% | T404N (ACT→AAT) | cusC → | copper/silver efflux system, outer membrane component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 596,033 | 0 | C | A | 9.0% | 208.9 / 5.0 | 66 | T404N (ACT→AAT) | cusC | copper/silver efflux system, outer membrane component |
| Reads supporting (aligned to +/- strand): ref base C (31/29); new base A (2/4); total (33/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.72e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
TGGCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGCTGGATGCCGAGCG > AP009048/595964‑596102 | tGGCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACt > 1:3514905/1‑71 (MQ=255)tGGCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACt > 1:3358192/1‑71 (MQ=255)tGGCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACt > 1:4009883/1‑71 (MQ=255)cggCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACt < 1:1901776/70‑1 (MQ=255) gCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACttt > 1:6352642/1‑71 (MQ=255) gCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACttt > 1:2072698/1‑71 (MQ=255) gCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACtt < 1:5299000/70‑1 (MQ=255) gCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACtt < 1:2284103/70‑1 (MQ=255) gCATTACGTCAAAGCCTGAACGATCAAACTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACttt > 1:2576518/1‑71 (MQ=255) cATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTg > 1:3980598/1‑71 (MQ=255) ttACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCa < 1:3864640/71‑1 (MQ=255) aCGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCaa > 1:3958934/1‑70 (MQ=255) cAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGgc > 1:5547358/1‑71 (MQ=255) aaaGCCTGAACGATCAAATTAGCGCCAAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGgcg > 1:6409482/1‑71 (MQ=255) gCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCgg < 1:7173435/71‑1 (MQ=255) gCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCgg < 1:3318244/71‑1 (MQ=255) gCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCgg < 1:6552179/71‑1 (MQ=255) cTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGTTGCAAAGTAATTTGCAGCga > 1:6915771/1‑62 (MQ=25) cTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGTTGCAAAGTAATTTGCAGCga > 1:3535287/1‑62 (MQ=25) aaCGATCAAATTAGCGCCCAGCAGCGTTATCTTGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCAtt < 1:6683464/71‑1 (MQ=255) aaCGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCAtt < 1:6499853/71‑1 (MQ=255) aCGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATta < 1:6865748/71‑1 (MQ=255) aCGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATta < 1:6392013/71‑1 (MQ=255) cGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATtat < 1:5202587/71‑1 (MQ=255) gATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGTTGCAAAGTAATTTGCAGCg < 1:874019/56‑1 (MQ=25) gATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGTTGCAAAGTAATTTGCAGCg < 1:3306117/56‑1 (MQ=25) gATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGTTGCAAAGTAATTTGCAGCg < 1:3196670/56‑1 (MQ=25) gATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGTTGCAAAGTAATTTGCAGCg < 1:7609151/56‑1 (MQ=25) gATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACt < 1:4879591/48‑1 (MQ=255) gATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACt < 1:2518660/48‑1 (MQ=255) aTCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTatat < 1:1304688/71‑1 (MQ=255) tCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATc < 1:3960053/71‑1 (MQ=255) cAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTatat > 1:4151418/1‑69 (MQ=255) aaaTTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAg > 1:6068348/1‑71 (MQ=255) aaaTTAGCGCCCACCAGCGTTATCTGGCATCGCTGCAAATTACTTTg > 1:4170647/1‑47 (MQ=255) aaTTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACttt > 1:7533301/1‑45 (MQ=255) aaTTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACttt > 1:3406035/1‑45 (MQ=255) cagcagCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTatat < 1:7731789/57‑1 (MQ=255) cagcGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTatat < 1:3659883/54‑1 (MQ=255) cagcGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAg > 1:2951240/1‑71 (MQ=255) gcGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGtt < 1:4474330/71‑1 (MQ=255) aTCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCt < 1:1023197/70‑1 (MQ=255) aTCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTg > 1:2620666/1‑71 (MQ=255) aTCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTg > 1:1567690/1‑71 (MQ=255) aTCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCA‑GGCGCAGTAAGTTATCTg > 1:5135076/1‑70 (MQ=255) tCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTg < 1:2436064/70‑1 (MQ=255) tCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTg < 1:4065360/70‑1 (MQ=255) tCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTg > 1:5587244/1‑70 (MQ=255) tGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGgcg > 1:4735622/1‑54 (MQ=255) gCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGgcg > 1:194468/1‑52 (MQ=255) gCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTgg < 1:5188235/67‑1 (MQ=255) gCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTgg < 1:5482397/67‑1 (MQ=255) aTCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTg > 1:1875117/1‑70 (MQ=255) aTCGCTGCAAATCACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGc < 1:5760480/71‑1 (MQ=255) tCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTgg < 1:1342302/64‑1 (MQ=255) tCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGCt > 1:1823231/1‑71 (MQ=255) cAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGCTGGAt < 1:3941568/69‑1 (MQ=255) aaaTTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGt > 1:4042549/1‑50 (MQ=255) aaaTTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGCTGGATGcc < 1:5316797/71‑1 (MQ=255) ttACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGCTGGATGc < 1:3237690/67‑1 (MQ=255) ttACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGCTGGATGCCGAg > 1:4797560/1‑71 (MQ=255) tACTTTGCAAGGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGt > 1:4294887/1‑46 (MQ=255) tACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGaa > 1:5499457/1‑55 (MQ=255) tACTTTGCAACGGGCGCGCGCATTATATCAGCACGGCGCAGTAAGt > 1:6543682/1‑46 (MQ=255) aCTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCt > 1:196434/1‑50 (MQ=255) aCTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCt > 1:1363338/1‑50 (MQ=255) cTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGCTGGATGCCGAGCg < 1:2146212/70‑1 (MQ=255) cTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGCTGGATGCCGAGCg < 1:1263163/70‑1 (MQ=255) | TGGCATTACGTCAAAGCCTGAACGATCAAATTAGCGCCCAGCAGCGTTATCTGGCATCGCTGCAAATTACTTTGCAACGGGCGCGGGCATTATATCAGCACGGCGCAGTAAGTTATCTGGAAGTGCTGGATGCCGAGCG > AP009048/595964‑596102 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |