| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,093,686 | T→C | 100% | V130A (GTA→GCA) | ycdT → | predicted diguanylate cyclase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,093,686 | 0 | T | C | 98.3% | 194.4 / ‑4.9 | 58 | V130A (GTA→GCA) | ycdT | predicted diguanylate cyclase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (22/35); minor base A (0/1); total (22/36) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.52e-01 | |||||||||||
| Rejected as polymorphism: E-value score below prediction cutoff. | |||||||||||
| Rejected as polymorphism: Frequency below/above cutoff threshold. | |||||||||||
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. | |||||||||||
TCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTG > AP009048/1093620‑1093752 | tCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTg < 1:2279002/71‑1 (MQ=255) gACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTg < 1:637812/63‑1 (MQ=255) aaaTTTCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATaa < 1:4710457/68‑1 (MQ=255) aCATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATaa < 1:3250611/70‑1 (MQ=255) cATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg < 1:3027538/71‑1 (MQ=255) ttGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt < 1:6998556/71‑1 (MQ=255) gCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAg > 1:1482259/1‑71 (MQ=255) aTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa < 1:6740082/71‑1 (MQ=255) aTCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa < 1:682528/71‑1 (MQ=255) tCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGa > 1:6166955/1‑70 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt > 1:7222829/1‑61 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaa < 1:6319778/67‑1 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAaca > 1:2108538/1‑71 (MQ=255) ttattTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAaca < 1:6172730/71‑1 (MQ=255) attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt < 1:1588293/58‑1 (MQ=255) attTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt < 1:1579130/58‑1 (MQ=255) tttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:920104/71‑1 (MQ=255) ttGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa > 1:5917849/1‑70 (MQ=255) tGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATa > 1:2052439/1‑51 (MQ=255) gTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa < 1:2482964/70‑1 (MQ=255) tCGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc > 1:438388/1‑71 (MQ=255) cGTCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaaaa > 1:353878/1‑66 (MQ=255) cGTCAGATGAGTTTGTGCATATTAATATTTCTGGAATTGGTGAATAAAGTTAGTGAAAACACAAAACAgc < 1:1682270/70‑1 (MQ=255) gTCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt < 1:6331043/37‑1 (MQ=255) gTCAGATGAGTTTGTGCATATTAATATTTCTGGCAtt < 1:5497664/37‑1 (MQ=255) tCAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt < 1:618783/49‑1 (MQ=255) cAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGtt < 1:7774823/48‑1 (MQ=255) cAGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGt < 1:5549714/47‑1 (MQ=255) aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAg > 1:5341013/1‑45 (MQ=255) aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAat > 1:3966253/1‑71 (MQ=255) aGATGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTG‑AAACACAAAACAGCGCAatt > 1:7013221/1‑71 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:1184964/1‑53 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:2266246/1‑53 (MQ=255) aTGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:3421671/58‑1 (MQ=255) tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:3241128/57‑1 (MQ=255) tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:1712067/57‑1 (MQ=255) tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta > 1:3991352/1‑71 (MQ=255) tGAGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt < 1:5034313/70‑1 (MQ=255) aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt < 1:892394/71‑1 (MQ=255) aGTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttat < 1:5811548/70‑1 (MQ=255) gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCaa > 1:7671216/1‑64 (MQ=255) gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttattt < 1:3698561/71‑1 (MQ=255) gTTTGTGCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttattt < 1:6444320/71‑1 (MQ=255) tgtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACaa < 1:2551466/51‑1 (MQ=255) gtgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAacac < 1:5238762/48‑1 (MQ=255) tgCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaa > 1:7614216/1‑43 (MQ=255) gCATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaa > 1:5924880/1‑70 (MQ=255) cATATTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattta < 1:6706340/61‑1 (MQ=255) atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGaaaa > 1:5217907/1‑41 (MQ=255) atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaa < 1:186888/71‑1 (MQ=255) atatTAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaa < 1:5977705/71‑1 (MQ=255) ttAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCaaaaaaa < 1:7576114/70‑1 (MQ=255) tAATATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAAt > 1:7449845/1‑70 (MQ=255) aaTATTTCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATg < 1:4843600/70‑1 (MQ=255) tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAatttatt > 1:4670310/1‑51 (MQ=255) tCTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAattt > 1:5957380/1‑48 (MQ=255) cTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtgtg > 1:5740485/1‑71 (MQ=255) cTGGCATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTtgt < 1:2923359/70‑1 (MQ=255) | TCCAAAATGACATTGCAATCTATTATTTGTTTCGTCAGATGAGTTTGTGCATATTAATATTTCTGGTATTGGTGAATAAAGTTAGTGAAAACACAAAACAGCGCAATTTATTTTCCAAAAAAATGACTTTGTG > AP009048/1093620‑1093752 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |