| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,185,346 | T→A | 5.1% | I226F (ATC→TTC) | potB ← | polyamine transporter subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,185,346 | 0 | T | A | 5.1% | 396.1 / 10.0 | 137 | I226F (ATC→TTC) | potB | polyamine transporter subunit |
| Reads supporting (aligned to +/- strand): ref base T (64/66); new base A (2/5); total (66/71) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.43e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
Only 100 of 145 total aligned reads displayed.
AATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCAC > AP009048/1185278‑1185412 | aTGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCTATcag < 1:5048588/71‑1 (MQ=255) aTGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcag < 1:1063512/71‑1 (MQ=255) aTGCTGATAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcag > 1:2917675/1‑71 (MQ=255) gCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcag > 1:2648589/1‑69 (MQ=255) gCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcag > 1:6630122/1‑69 (MQ=255) cTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcagca < 1:4363459/70‑1 (MQ=255) tAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGttt < 1:4448920/71‑1 (MQ=255) tAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGttt < 1:7663037/71‑1 (MQ=255) gCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGtttt < 1:3375059/70‑1 (MQ=255) tGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGttttt < 1:7828323/69‑1 (MQ=255) tGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGttttt < 1:5445761/69‑1 (MQ=255) aCCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcagca < 1:7662626/59‑1 (MQ=255) aCCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCAc > 1:6454332/1‑71 (MQ=255) ccAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcag < 1:1773543/56‑1 (MQ=255) cAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGttttt > 1:6319765/1‑64 (MQ=255) cAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCAc < 1:424488/69‑1 (MQ=255) cAAACGGCCACTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAgg > 1:5623728/1‑59 (MQ=255) aaaCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcagca > 1:5376267/1‑56 (MQ=255) aaaCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcagca > 1:7665496/1‑56 (MQ=255) aaaCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcagca > 1:6004068/1‑56 (MQ=255) aCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcagcag > 1:2846362/1‑55 (MQ=255) aCGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGcc > 1:4117871/1‑70 (MQ=255) cGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGt < 1:3351725/56‑1 (MQ=255) ggCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCAt < 1:6399367/71‑1 (MQ=255) gCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATc < 1:1107604/71‑1 (MQ=255) tctgTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATc < 1:736343/67‑1 (MQ=255) ccAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGtt < 1:7221626/54‑1 (MQ=255) ccAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGc < 1:1064715/65‑1 (MQ=255) ccAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCAt > 1:2978063/1‑69 (MQ=255) ccAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCAt > 1:7846186/1‑69 (MQ=255) aGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCAcc > 1:2511085/1‑61 (MQ=255) aGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCa < 1:3404986/66‑1 (MQ=255) aGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCa < 1:1218314/66‑1 (MQ=255) gTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGttt < 1:1971331/52‑1 (MQ=255) gTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCg < 1:2167431/56‑1 (MQ=255) tCACGAATATTAAGGAACTGGACCTTGATGACGTTACCTATCAGCAGGtttt < 1:1282936/52‑1 (MQ=255) tCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATc < 1:4230438/66‑1 (MQ=255) tCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATc < 1:3210868/66‑1 (MQ=255) cACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcagca > 1:6630371/1‑45 (MQ=255) cACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcagca > 1:4876059/1‑45 (MQ=255) cACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATcagca > 1:1319084/1‑45 (MQ=255) aCGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGt < 1:3291270/47‑1 (MQ=255) cGAATATTAAGGAACTGGACCTTGATGACGTTACCGAt > 1:6857389/1‑38 (MQ=255) cGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGa < 1:5084099/71‑1 (MQ=255) cGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGa < 1:2580498/71‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGAt < 1:4974822/37‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGAt < 1:7489163/37‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGAt < 1:4434983/37‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGAt < 1:4185750/37‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCAc > 1:2942609/1‑54 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCAc > 1:5795537/1‑54 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGAt < 1:4010983/71‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGAt < 1:4566448/71‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGAt < 1:4963293/71‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGAt < 1:2778920/71‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGAACAGCAgg < 1:6814936/44‑1 (MQ=255) gAATATTAAGGAACTGGACCTTGATGACGTTACCGAACAGCAgg < 1:7786117/44‑1 (MQ=255) aaTATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATa < 1:3722270/71‑1 (MQ=255) atatTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCa > 1:588474/1‑51 (MQ=255) atatTAAGGAACTGGACCTTGATGACGTTACCGAACAGCAGGtt > 1:1544494/1‑44 (MQ=255) atatTAAGGAACTGGACCTTGATGACGTTACCGAACAGCAGGtt > 1:3291089/1‑44 (MQ=255) tatTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACa < 1:1867920/71‑1 (MQ=255) tatTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACa < 1:7071559/71‑1 (MQ=255) ttAAGGAACTGGACCTTGATGACGTTACCGATcagca < 1:7079402/37‑1 (MQ=255) ggAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACAt > 1:7353561/1‑66 (MQ=255) aaCTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAg > 1:1340786/1‑71 (MQ=255) aCTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAgg < 1:5432432/71‑1 (MQ=255) cTGGACCTTGATGACGTTACCGATCAGCAGGTTTATCGCACCGCCCATCAGGTc < 1:3482785/54‑1 (MQ=255) tGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCg > 1:5352912/1‑37 (MQ=255) tGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCg > 1:7753609/1‑37 (MQ=255) tGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCg > 1:7744427/1‑37 (MQ=255) tGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGcc > 1:7137706/1‑71 (MQ=255) ggACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCg > 1:5053051/1‑41 (MQ=255) ggACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATa > 1:6195563/1‑61 (MQ=255) ccTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAgg > 1:1413446/1‑65 (MQ=255) ccTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAgg > 1:1711190/1‑65 (MQ=255) cTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCAt < 1:3729708/42‑1 (MQ=255) cTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCAt < 1:2162080/42‑1 (MQ=255) ttGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGc > 1:3170677/1‑71 (MQ=255) tgatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCgg > 1:3811504/1‑48 (MQ=255) tgatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCgg > 1:4168422/1‑48 (MQ=255) tgatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCgg > 1:2039676/1‑48 (MQ=255) tgatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCgg > 1:7114404/1‑48 (MQ=255) tgatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCgg > 1:3572813/1‑48 (MQ=255) tgatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCt > 1:984023/1‑71 (MQ=255) gatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGc < 1:1537696/69‑1 (MQ=255) gatgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCt < 1:3125313/70‑1 (MQ=255) atgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTg < 1:864214/70‑1 (MQ=255) tgaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGgc < 1:7723694/71‑1 (MQ=255) gaCGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACAt < 1:6533861/50‑1 (MQ=255) cGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAg < 1:357830/37‑1 (MQ=255) gTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGgcagca > 1:1499849/1‑71 (MQ=255) tACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAgg > 1:6476827/1‑53 (MQ=255) tACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAgg > 1:6244862/1‑53 (MQ=255) ccGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCAc > 1:2311190/1‑71 (MQ=255) aTCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACAt > 1:1935207/1‑40 (MQ=255) aTCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACAt > 1:5820450/1‑40 (MQ=255) aTCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGaa > 1:7490510/1‑44 (MQ=255) aTCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGaa > 1:5117847/1‑44 (MQ=255) aTCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGgc > 1:135791/1‑60 (MQ=255) | AATGCTGGTAGCTGCACCAAACGGCCAGTCACGAATATTAAGGAACTGGACCTTGATGACGTTACCGATCAGCAGGTTTTTCGCACCGCCCATCAGGTCGGATACATAGAACAGGCCCATCGCTGGCAGCATCAC > AP009048/1185278‑1185412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |