| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,250,324 | T→A | 5.8% | D123V (GAC→GTC) | dhaH ← | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,250,324 | 0 | T | A | 5.8% | 293.7 / 8.2 | 104 | D123V (GAC→GTC) | dhaH | fused predicted dihydroxyacetone‑specific PTS enzyme HPr component and EI component |
| Reads supporting (aligned to +/- strand): ref base T (50/48); new base A (3/3); total (53/51) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
Only 100 of 106 total aligned reads displayed.
AGAGATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGAC > AP009048/1250257‑1250391 | agagATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCa > 1:3847043/1‑70 (MQ=255)agagATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCa < 1:1501998/70‑1 (MQ=255) gagaTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCa > 1:2378054/1‑69 (MQ=255) agaTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAg < 1:436550/71‑1 (MQ=255) agaTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAg < 1:6837382/71‑1 (MQ=255) gaTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCa > 1:3890039/1‑67 (MQ=255) gaTTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCa > 1:2898535/1‑67 (MQ=255) ttCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa > 1:7766216/1‑71 (MQ=255) tCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa < 1:179899/70‑1 (MQ=255) tCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa < 1:1660837/70‑1 (MQ=255) tCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa < 1:2764037/70‑1 (MQ=255) aGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACtt > 1:2275459/1‑71 (MQ=255) aGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACtt > 1:3358083/1‑71 (MQ=255) aGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt < 1:7008566/70‑1 (MQ=255) aGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCAAGCGCATGCATGGCGTCAAAGATAACt < 1:7015524/70‑1 (MQ=255) aGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTACCAGCGCATGCATGGCGTCaaa < 1:7751085/63‑1 (MQ=255) gtggCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACttt < 1:4290107/71‑1 (MQ=255) tgtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGa > 1:1062619/1‑63 (MQ=255) tgtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATa > 1:5311285/1‑65 (MQ=255) tgtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATa > 1:6036363/1‑65 (MQ=255) tgtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTg < 1:2952593/71‑1 (MQ=255) tgtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTg < 1:4635861/71‑1 (MQ=255) gtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTg > 1:447149/1‑70 (MQ=255) gtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt > 1:2292102/1‑71 (MQ=255) gtCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt > 1:210586/1‑71 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGGGCATGCATGGCGTCAAAGATAACTTTGTc > 1:1084740/1‑71 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATATCTTTGTc > 1:5482042/1‑71 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt < 1:7320531/70‑1 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt < 1:6583428/70‑1 (MQ=255) tCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt < 1:7604461/70‑1 (MQ=255) cGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTc > 1:1706385/1‑70 (MQ=255) gaggaCGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGat < 1:2749459/71‑1 (MQ=255) ggaCGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGatat < 1:4889147/71‑1 (MQ=255) ggaCGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGatat < 1:4503497/71‑1 (MQ=255) cGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCg > 1:1394334/1‑71 (MQ=255) cGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCg > 1:2608125/1‑71 (MQ=255) gTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa < 1:2313013/54‑1 (MQ=255) gTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGcc > 1:1909805/1‑71 (MQ=255) tAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccc > 1:1754914/1‑71 (MQ=255) tAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccc > 1:3968818/1‑71 (MQ=255) tAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccc > 1:5520827/1‑71 (MQ=255) tAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccc > 1:3890056/1‑71 (MQ=255) aaaCCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATc > 1:5868368/1‑65 (MQ=255) aCCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATaa > 1:2868928/1‑50 (MQ=255) cccAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGa < 1:1394056/71‑1 (MQ=255) ccAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGa < 1:6178527/70‑1 (MQ=255) ccAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAg < 1:791887/71‑1 (MQ=255) ccAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAg < 1:1263219/71‑1 (MQ=255) aGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAgg > 1:5693256/1‑70 (MQ=255) aGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGc < 1:2220712/71‑1 (MQ=255) aGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGc < 1:4985820/71‑1 (MQ=255) ttgttCACGTTTGGCTTCCAGCGCATGCATGGCGTc < 1:2388295/36‑1 (MQ=255) ttgttCACGTTTGGCTTCCAGCGCATGCATGGCGTc < 1:6530815/36‑1 (MQ=255) ttgttCACGTTTGGCTTCCAGCGCATGCATGGCGTc < 1:1535484/36‑1 (MQ=255) ttgttCACGTTTGGCTTCCAGCGCATGCATGGCGTCa < 1:6873049/37‑1 (MQ=255) ttgttCACGTTTGGCTTCCAGCGCATGCATGGCGTCa < 1:2407970/37‑1 (MQ=255) gttCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt < 1:5590474/44‑1 (MQ=255) gttCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt < 1:5453100/44‑1 (MQ=255) gttCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt < 1:3652069/44‑1 (MQ=255) gttCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACt < 1:2409381/44‑1 (MQ=255) ttCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCgcgc < 1:3113135/71‑1 (MQ=255) tCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCt > 1:6840308/1‑71 (MQ=255) tCACGTTTGGCTTCCAGCGCATGCATGGCGACAAAGTTATCt < 1:27713/42‑1 (MQ=37) aCGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGat < 1:4275096/48‑1 (MQ=255) aCGTTTGGCTTCCAGCGCATGCATGGCGACAAAGTTATCttt > 1:1883557/1‑42 (MQ=25) aCGTTTGGCTTCCAGCGCATGCATGGCGACAAAGTTATCttt > 1:1488091/1‑42 (MQ=25) aCGTTTGGCTTCCAGCGCATGCATGGCGACAAAGTTATCttt > 1:2289224/1‑42 (MQ=25) cGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGAc > 1:7824322/1‑71 (MQ=255) tttGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTg < 1:5930286/40‑1 (MQ=255) tttGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCg < 1:2977470/71‑1 (MQ=255) tGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGt > 1:193006/1‑39 (MQ=255) tGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTc > 1:1973741/1‑40 (MQ=255) tGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGAcc > 1:5112412/1‑68 (MQ=255) cTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGccccc < 1:1280106/50‑1 (MQ=255) ttCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGa > 1:33852/1‑38 (MQ=255) ttCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGa > 1:228234/1‑38 (MQ=255) tCCAGCGCATGCATGGCGTCAAAGATAACTTTGTc > 1:4183617/1‑35 (MQ=255) tCCAGCGCATGCATGGCGTCAAAGATAACTTTGTc > 1:2180010/1‑35 (MQ=255) tCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGa > 1:547179/1‑50 (MQ=255) tCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGAc > 1:1608813/1‑62 (MQ=255) ccAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTtgctg < 1:1436459/69‑1 (MQ=255) aGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAg > 1:4524779/1‑71 (MQ=255) gcatgcatGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCg < 1:1627581/58‑1 (MQ=255) atgcatgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCg > 1:6161633/1‑41 (MQ=255) tgcatgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTAcc < 1:3966548/71‑1 (MQ=255) gcatgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTAcc < 1:4830609/70‑1 (MQ=255) gcatgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCt > 1:1910279/1‑71 (MQ=255) catgGCGTCAAAGATAACTTTGTCGATATCCGccccc < 1:6364699/37‑1 (MQ=255) catgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCt < 1:6617587/70‑1 (MQ=255) atgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAg > 1:5931822/1‑62 (MQ=255) atgGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTc > 1:1075832/1‑71 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAgg > 1:2899143/1‑38 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGt > 1:6260086/1‑51 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGt > 1:1569647/1‑51 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAgtgt > 1:4295170/1‑63 (MQ=255) ggCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGa > 1:4990305/1‑71 (MQ=255) gCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGAc > 1:1911695/1‑71 (MQ=255) gTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAgt < 1:1891971/58‑1 (MQ=255) tCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCctcg < 1:5990347/39‑1 (MQ=255) tCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCcgcg < 1:1668208/39‑1 (MQ=255) | AGAGATTTCAGTGTCGGAGGACGGTAAACCCAGTTGTTCACGTTTGGCTTCCAGCGCATGCATGGCGTCAAAGATAACTTTGTCGATATCCGCCCCCGAGGCCGCGCTGACCGTTGCTGCCAGTGTACCTTCGAC > AP009048/1250257‑1250391 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |