| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 71,109 | T→C | 75.2% | T241T (ACT→ACC) | araC → | DNA‑binding transcriptional dual regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 71,109 | 0 | T | C | 75.2% | 792.0 / 276.2 | 416 | T241T (ACT→ACC) | araC | DNA‑binding transcriptional dual regulator |
| Reads supporting (aligned to +/- strand): ref base T (60/43); new base C (159/153); total (220/196) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.12e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.27e-01 | |||||||||||
Only 100 of 436 total aligned reads displayed.
AGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCG > AP009048/71043‑71174 | aGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCAccc > 1:4394920/1‑71 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg < 1:5471020/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg < 1:6040258/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg > 1:5030085/1‑71 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg < 1:4920564/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCg < 1:7893767/71‑1 (MQ=255) gggATTAGCGTCTTAAGCTGGCGCGAGGACAAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg < 1:6474871/71‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCgg < 1:4726727/71‑1 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCg > 1:172726/1‑70 (MQ=255) ggATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCg > 1:297640/1‑70 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGAt < 1:2576202/71‑1 (MQ=255) aTTAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGAt < 1:2629877/71‑1 (MQ=255) ttAGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGAt < 1:2676420/70‑1 (MQ=255) tAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGc > 1:5389062/1‑71 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGcc > 1:2187817/1‑71 (MQ=255) aGCGTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGc < 1:3998877/70‑1 (MQ=255) gTCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTAt < 1:2494046/71‑1 (MQ=255) tCTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCt < 1:6935488/68‑1 (MQ=255) cTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGa > 1:3922359/1‑62 (MQ=255) cTTAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCg > 1:2030299/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGcc < 1:5500378/65‑1 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCt > 1:4591260/1‑66 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc > 1:1685020/1‑71 (MQ=255) ttAAGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGc > 1:3780122/1‑71 (MQ=255) ttAAGCTGGCGCGAGGAACAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGAt > 1:6407219/1‑62 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCg < 1:1791919/57‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGcc < 1:6240638/70‑1 (MQ=255) aaGCTGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCa > 1:5294493/1‑71 (MQ=255) cTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCAcc < 1:1300384/70‑1 (MQ=255) tGGCGCGAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCt > 1:2728354/1‑60 (MQ=255) cgcgAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcgg < 1:738212/71‑1 (MQ=255) cgcgAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcgg < 1:3373550/71‑1 (MQ=255) gcgAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggt > 1:7540194/1‑71 (MQ=255) gcgAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggt < 1:7277090/71‑1 (MQ=255) gAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggtcg > 1:4216138/1‑71 (MQ=255) gAGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggtcg > 1:4671035/1‑71 (MQ=255) gAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTAt < 1:3513786/56‑1 (MQ=255) gAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggtcg > 1:2996399/1‑71 (MQ=255) gAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCG‑CACCgtcggtcg > 1:290299/1‑70 (MQ=255) aGGACCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcggtcg > 1:3422415/1‑70 (MQ=255) aCCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCaccacc < 1:6550739/41‑1 (MQ=25) aCCAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGc < 1:7601427/68‑1 (MQ=255) ccAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGa > 1:948824/1‑44 (MQ=25) ccAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg > 1:7804630/1‑71 (MQ=255) cAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt < 1:3951936/71‑1 (MQ=255) cAACGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGt > 1:6832125/1‑71 (MQ=255) aaCGTATCAGCCAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt < 1:4804829/71‑1 (MQ=255) aCGTATCAGCCAGGCGGAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGtt > 1:3738448/1‑71 (MQ=255) cGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtcggt < 1:7746011/60‑1 (MQ=255) cGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCTCAATGTTg > 1:3324834/1‑70 (MQ=255) cATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtt < 1:7633107/71‑1 (MQ=255) ttAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt < 1:6609519/71‑1 (MQ=255) gTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATc < 1:4983126/41‑1 (MQ=255) gTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc < 1:6354060/71‑1 (MQ=255) cAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCg > 1:7502530/1‑40 (MQ=255) cAGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCg > 1:2099569/1‑40 (MQ=255) aGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGAt > 1:5758070/1‑71 (MQ=255) aGGC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGAt > 1:5869380/1‑71 (MQ=255) ggC‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACTATc < 1:964483/71‑1 (MQ=255) c‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCaa < 1:1109654/71‑1 (MQ=255) c‑GAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCaa < 1:4042381/71‑1 (MQ=255) gAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtc < 1:125315/43‑1 (MQ=255) gAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCgtc < 1:5662324/43‑1 (MQ=255) gAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTg < 1:5155318/56‑1 (MQ=255) gAAGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAAc > 1:410248/1‑71 (MQ=255) aaGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCgtcg < 1:5927982/43‑1 (MQ=255) aaGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAAct < 1:7005277/71‑1 (MQ=255) aaGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAAc > 1:4295560/1‑70 (MQ=255) aGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCaa > 1:7154856/1‑49 (MQ=255) aGCTGCTTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAAct > 1:4444471/1‑70 (MQ=255) gctgctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActct < 1:6049310/71‑1 (MQ=255) gctgctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAActct < 1:1927013/71‑1 (MQ=255) gctTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATg > 1:7553661/1‑47 (MQ=255) gctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATg < 1:7765482/47‑1 (MQ=255) gctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGtttt > 1:3162983/1‑55 (MQ=255) gctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTa > 1:1867529/1‑69 (MQ=255) ctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAttt > 1:2323859/1‑71 (MQ=255) ctTTTGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAt < 1:1566917/69‑1 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCACTGTTgg < 1:1012915/49‑1 (MQ=255) ttttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTgg < 1:2807143/49‑1 (MQ=255) tttGAGCACCACCCGGATGCCTATCGCAACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtct > 1:6731382/1‑71 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCGATGTTGGTTTTg > 1:5169115/1‑52 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGa > 1:7062309/1‑56 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCaa > 1:2286617/1‑60 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCaa > 1:3080523/1‑60 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTAttt < 1:7449742/68‑1 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtc < 1:6135319/69‑1 (MQ=255) ttGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtc < 1:1504237/69‑1 (MQ=255) tGAGCACCACCCGGATGCCTATCGCCACCgtcggtc > 1:2220300/1‑36 (MQ=255) tGAGCACCACCCGGATGCCTATCGCCACCgtcggtc > 1:6309608/1‑36 (MQ=255) tGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGa > 1:5244955/1‑52 (MQ=255) tGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc > 1:6158366/1‑53 (MQ=255) tGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGAc > 1:3855067/1‑53 (MQ=255) tGAGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTtctc < 1:1167296/70‑1 (MQ=255) aGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgcg < 1:4570889/71‑1 (MQ=255) aGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgcg < 1:3656678/71‑1 (MQ=255) aGCACCCCCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgc < 1:7733336/70‑1 (MQ=255) aGCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTcgc < 1:815320/70‑1 (MQ=255) gCACCACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATc > 1:1382353/1‑54 (MQ=255) cACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTa > 1:120378/1‑60 (MQ=255) | AGGGATTAGCGTCTTAAGCTGGCGCGAGGACCAACGCATTAGTCAGGC‑GAAGCTGCTTTTGAGCACTACCCGGATGCCTATCGCCACCGTCGGTCGCAATGTTGGTTTTGACGATCAACTCTATTTCTCGCG > AP009048/71043‑71174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |