| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,634,421 | T→A | 5.7% | intergenic (‑422/‑332) | ydfJ ← / → ydfK | predicted transporter/predicted DNA‑binding transcriptional regulator |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,634,421 | 0 | T | A | 5.7% | 195.3 / 3.6 | 70 | intergenic (‑422/‑332) | ydfJ/ydfK | predicted transporter/predicted DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (37/29); new base A (2/2); total (39/31) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CCTTGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGATTTTTGCTGC > AP009048/1634352‑1634486 | ccTTGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGtc < 1:968438/71‑1 (MQ=34) cTTGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGtct > 1:6165124/1‑71 (MQ=34) tGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGtctc > 1:1156863/1‑70 (MQ=35) gATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTa > 1:2136698/1‑71 (MQ=35) tttttctttttcAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGACTCTACac < 1:2075235/70‑2 (MQ=255) tttttcAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATGAGATAg > 1:590980/1‑71 (MQ=255) tttttcAGATAAAAACTGGTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAg > 1:7159736/1‑71 (MQ=255) tttcAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTctct > 1:5955414/1‑58 (MQ=31) tttcAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGtt > 1:1269330/1‑71 (MQ=35) tttcAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGtt > 1:1046694/1‑71 (MQ=35) tcAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTg > 1:367526/1‑71 (MQ=34) tcAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTg > 1:3512238/1‑71 (MQ=34) tcAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTg > 1:2421049/1‑71 (MQ=34) cAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTg < 1:5917985/70‑1 (MQ=35) gATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAg < 1:3054039/71‑1 (MQ=34) gATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAg < 1:4363383/71‑1 (MQ=34) gATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAg < 1:3063060/71‑1 (MQ=34) gATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAg < 1:5314806/71‑1 (MQ=34) tAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGAt < 1:2995601/60‑1 (MQ=35) tAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGtt > 1:5016317/1‑71 (MQ=34) tAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGtt > 1:1526163/1‑71 (MQ=34) aCTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGc > 1:6550882/1‑71 (MQ=38) aCTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGc > 1:7580588/1‑71 (MQ=38) aCTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGc < 1:7567486/71‑1 (MQ=38) tATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCa > 1:5828848/1‑58 (MQ=35) tGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTg < 1:4132818/69‑1 (MQ=40) tGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTg < 1:5740498/69‑1 (MQ=40) gTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGa < 1:3584516/42‑1 (MQ=38) gTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAg > 1:7710688/1‑52 (MQ=35) gTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTg > 1:6489718/1‑55 (MQ=35) tataCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCt < 1:3271005/59‑1 (MQ=255) tataCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCt < 1:4804965/59‑1 (MQ=255) tataCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAg < 1:1384925/63‑1 (MQ=40) ataCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCt < 1:5236554/64‑1 (MQ=40) ataCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCa > 1:1304294/1‑71 (MQ=39) ataCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCa > 1:3901986/1‑71 (MQ=39) ataCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCa > 1:1313478/1‑71 (MQ=39) taCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCtt > 1:7061813/1‑58 (MQ=255) taCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCtt > 1:256481/1‑58 (MQ=255) taCTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCt < 1:2127867/63‑1 (MQ=40) cTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATaa < 1:4256262/71‑1 (MQ=255) cTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATa < 1:6844599/70‑1 (MQ=255) ttttAAACCCAATCCGTGTAGAGTCTCTACATAAGATAg > 1:5949754/1‑39 (MQ=40) ttttAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGt > 1:119543/1‑40 (MQ=40) ttttAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGt > 1:2252510/1‑40 (MQ=40) ttttAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCtt < 1:6516683/61‑1 (MQ=255) ttAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCtt < 1:6486269/53‑1 (MQ=255) ttAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTgcgc > 1:6958429/1‑63 (MQ=255) ttAAACACAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATa > 1:6983182/1‑67 (MQ=255) tAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGc > 1:7587216/1‑56 (MQ=255) tAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCg > 1:4809917/1‑71 (MQ=255) aCCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAg > 1:5028117/1‑40 (MQ=40) aCCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAg > 1:3672732/1‑40 (MQ=40) aCCCAATCAGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCt > 1:61142/1‑54 (MQ=255) ctaTCCGTTTAGAGTCTCTACATAAGATAGTTTGCTGTTGCCGCTTCa < 1:5692034/46‑1 (MQ=14) aaTCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGc < 1:3499455/43‑1 (MQ=255) aaTCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGAtt > 1:3620760/1‑71 (MQ=255) aaTCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGAtt > 1:127820/1‑71 (MQ=255) aTCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGtt < 1:6653760/37‑1 (MQ=40) aTCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGtt < 1:997799/37‑1 (MQ=40) aTCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGtt < 1:4869016/37‑1 (MQ=40) aTCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGAttt < 1:3772201/71‑1 (MQ=39) aTCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGAttt < 1:1190062/71‑1 (MQ=39) ccGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGc > 1:1872866/1‑37 (MQ=40) tatgtAGAGACTCTACACAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCt > 1:3676618/3‑63 (MQ=14) tatgtAGAGACTCTACACAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGa > 1:2783092/3‑65 (MQ=14) gtgtAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGATTTTtgc > 1:639476/1‑71 (MQ=39) gtgtAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGATTTTtgc > 1:3896120/1‑71 (MQ=39) atgtAGAGACTCTACACAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGAttt < 1:3172607/66‑1 (MQ=14) tgtAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATaaa < 1:2182785/55‑1 (MQ=255) tgtAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATaaa < 1:299006/55‑1 (MQ=255) agagTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGATTTTtgctgc > 1:7307516/1‑70 (MQ=255) gagTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGATTTTtgc < 1:6214554/66‑1 (MQ=40) tctcTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATa < 1:540802/46‑1 (MQ=255) | CCTTGATATTTTTCTTTTTCAGATAAAAACTGTTATCTATGTATACTTTTAAACCCAATCCGTGTAGAGTCTCTACATAAGATAGTTTGCAGTTGCCGCTTCAGCTTGCGCCATAAACCGCCTGATTTTTGCTGC > AP009048/1634352‑1634486 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |