| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | AP009048 | 1,894,072 | C→T | 5.6% | G293D (GGT→GAT) | yoaA ← | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | AP009048 | 1,894,072 | 0 | C | T | 5.6% | 344.6 / 7.0 | 107 | G293D (GGT→GAT) | yoaA | conserved hypothetical protein with nucleoside triphosphate hydrolase domain |
| Reads supporting (aligned to +/- strand): ref base C (47/53); new base T (4/2); total (51/56) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.25e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01 | |||||||||||
Only 100 of 109 total aligned reads displayed.
TTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCAC > AP009048/1894002‑1894140 | ttCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTAc > 1:3551967/1‑71 (MQ=255) gtgtCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATa < 1:6562956/71‑1 (MQ=255) tgtCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACcg > 1:5674757/1‑65 (MQ=255) tCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGAt > 1:2034727/1‑67 (MQ=255) tCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAAc < 1:226884/70‑1 (MQ=255) aTCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCt < 1:6840635/70‑1 (MQ=255) aTCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTg > 1:4168154/1‑71 (MQ=255) aTCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTg > 1:1594494/1‑71 (MQ=255) tCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTgg > 1:3214245/1‑71 (MQ=255) cGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGc > 1:5925818/1‑71 (MQ=255) gTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTAc < 1:2341815/51‑1 (MQ=255) tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACcgcg < 1:4232065/54‑1 (MQ=255) tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGAt < 1:1047389/56‑1 (MQ=255) tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGAt < 1:1413541/56‑1 (MQ=255) tAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTg < 1:707406/62‑1 (MQ=255) aaaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAg > 1:3531643/1‑71 (MQ=255) aaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTg > 1:3355634/1‑60 (MQ=255) aaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTg > 1:2014350/1‑60 (MQ=255) aaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGc > 1:7337298/1‑62 (MQ=255) aaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGt > 1:2573817/1‑71 (MQ=255) aaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGt > 1:849128/1‑71 (MQ=255) aaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGt > 1:64905/1‑71 (MQ=255) aaaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGt > 1:2274406/1‑71 (MQ=255) aaaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGtt < 1:7404179/71‑1 (MQ=255) aaTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCAc < 1:7164441/64‑1 (MQ=255) aTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTg > 1:5723860/1‑70 (MQ=255) aTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTg > 1:5525724/1‑70 (MQ=255) aTGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGc < 1:5793724/71‑1 (MQ=255) tGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCt < 1:4734079/55‑1 (MQ=255) cccGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGa < 1:5738999/71‑1 (MQ=255) cccGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGa < 1:3602014/71‑1 (MQ=255) gCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCAcc < 1:1972638/58‑1 (MQ=255) gCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACg > 1:543348/1‑70 (MQ=255) gCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACg > 1:918541/1‑70 (MQ=255) tGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTATcgcg < 1:2864746/41‑1 (MQ=255) gAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACcgcg < 1:4858716/40‑1 (MQ=255) gAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACcgcg < 1:3799555/40‑1 (MQ=255) gAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAAcc < 1:1172613/46‑1 (MQ=255) gAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAAcc < 1:1290441/46‑1 (MQ=255) gAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGaa < 1:1835964/69‑1 (MQ=255) aaTTTGCGGATTAGCTAACAGCTCACGCAGGTTATcgcggt > 1:439278/1‑39 (MQ=39) aaTTTGCGGATTAGCTAACAGCTCACGCAGGTTATcgcggt > 1:6463714/1‑39 (MQ=39) aaTTTGCGGATTAGCTAACAGCTCACGCAGGTTATcgcggt > 1:7637168/1‑39 (MQ=39) aaTTTGCGGATTAGCTAACAGCTCACGCAGGTTATcgcggt > 1:3978366/1‑39 (MQ=39) aaTTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGaaaa < 1:4708944/70‑1 (MQ=255) aTTTGCGGATTAGCTAACAGCTCACGCAGGTTACcg > 1:4040470/1‑36 (MQ=255) aTTTGCGGATTAGCTAACAGCTCACGCAGGTTACcg > 1:1226960/1‑36 (MQ=255) aTTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATc < 1:244987/71‑1 (MQ=255) tttGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGAc > 1:2792172/1‑63 (MQ=255) tttGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGaa > 1:7476306/1‑66 (MQ=255) tttGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATcc > 1:7922475/1‑71 (MQ=255) tttGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATcc > 1:120683/1‑71 (MQ=255) tGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCt < 1:5990634/70‑1 (MQ=255) gCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTg < 1:1761756/70‑1 (MQ=255) gATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCa > 1:2312491/1‑71 (MQ=255) aTTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCAc > 1:6120007/1‑71 (MQ=255) ttAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCAct > 1:3604734/1‑71 (MQ=255) tAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCa > 1:5940023/1‑51 (MQ=255) tAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCActc > 1:2943314/1‑71 (MQ=255) tAGCTAACAGCTCACGAAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCActc > 1:4248/1‑71 (MQ=255) aGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCActc < 1:2014086/70‑1 (MQ=255) gCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCActct < 1:1716188/70‑1 (MQ=255) gCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCActct < 1:5337205/70‑1 (MQ=255) gCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTg < 1:3424302/71‑1 (MQ=255) cTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAg < 1:7182468/43‑1 (MQ=255) cTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATc < 1:5638870/58‑1 (MQ=255) aaCAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCActc < 1:3025799/66‑1 (MQ=255) aCAGCTCACGCAGGTTAGCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTg < 1:5054149/67‑1 (MQ=255) aCAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGc < 1:3759803/70‑1 (MQ=255) cAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCaa < 1:1191487/71‑1 (MQ=255) aGCTTACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTg < 1:5517036/56‑1 (MQ=255) aGCTCACGCAGGTTACCGCGATAACCTGGCTCACCg > 1:614970/1‑36 (MQ=255) aGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTg < 1:1245278/56‑1 (MQ=255) aGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTg < 1:7887156/56‑1 (MQ=255) gCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGa < 1:3010725/71‑1 (MQ=255) tCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGa > 1:316608/1‑45 (MQ=255) tCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCaa < 1:3208127/67‑1 (MQ=255) tCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGAc > 1:4304933/1‑70 (MQ=255) cACGCAGGTTACCGCGATAACCTGGCTCACGGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGa > 1:7773749/1‑71 (MQ=255) aCGCAGGTTGCCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACg < 1:2050321/69‑1 (MQ=255) aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACg < 1:414878/69‑1 (MQ=255) aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACg < 1:4567176/69‑1 (MQ=255) aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGAt < 1:351499/71‑1 (MQ=255) aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGAt < 1:342174/71‑1 (MQ=255) aCGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGAt < 1:1808657/71‑1 (MQ=255) cGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGAt > 1:3872531/1‑70 (MQ=255) gCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCa < 1:4790747/53‑1 (MQ=255) gCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCa < 1:2525572/53‑1 (MQ=255) cAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAg > 1:7023318/1‑71 (MQ=255) aGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGa > 1:3129682/1‑36 (MQ=255) aGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGa > 1:7654250/1‑36 (MQ=255) aGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGa > 1:3502387/1‑36 (MQ=255) ggTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGa < 1:6823287/38‑1 (MQ=255) gTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCAct < 1:5896421/51‑1 (MQ=255) gTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGAt < 1:746515/65‑1 (MQ=255) tACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCa > 1:3030759/1‑65 (MQ=255) tACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCAc > 1:1579375/1‑71 (MQ=255) tACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCAc > 1:511649/1‑71 (MQ=255) aCCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCa > 1:6969927/1‑64 (MQ=255) aCCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCAc < 1:6363165/70‑1 (MQ=255) | TTCCAGGGTGTCATCGAGCAGTAAAAATGCCCGCTGAATTTGCGGATTAGCTAACAGCTCACGCAGGTTACCGCGATAACCTGGCTCACCGAGTTGCAGACGAAAATCCTGCGCACTCTGGGCAAGACGATCAGCGCAC > AP009048/1894002‑1894140 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |