Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,467,882 Δ1 bp 28.6% intergenic (+41/+87) ubiG → / ← yfaL bifunctional 3‑demethylubiquinone‑9 3‑methyltransferase and 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,467,8820C.28.6% 112.9 / 43.5 49intergenic (+41/+87)ubiG/yfaLbifunctional 3‑demethylubiquinone‑9 3‑methyltransferase and 2‑octaprenyl‑6‑hydroxy phenol methylase/adhesin
Reads supporting (aligned to +/- strand):  ref base C (18/16);  new base . (7/7);  total (26/23)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

AACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGC  >  Exported/1467812‑1467946
                                                                      |                                                                      
aaCTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGc                                                                        >  1:1855911/1‑71 (MQ=255)
     tatGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCcgc                                                                     <  1:1572228/69‑1 (MQ=255)
     tatGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGt                                                                   >  1:3275972/1‑71 (MQ=255)
       tgctgcACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                 <  1:4200223/71‑1 (MQ=255)
             acacGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATTCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                           <  1:1863481/71‑1 (MQ=255)
             acacGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                              >  1:4636442/1‑68 (MQ=255)
             acacGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAg                                                           <  1:2071342/71‑1 (MQ=255)
                    gAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTAc                                                     >  1:72546/1‑70 (MQ=255)
                      aTAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACaa                                                   >  1:3396830/1‑70 (MQ=255)
                       tAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                               <  1:135197/57‑1 (MQ=255)
                       tAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTa                                                               <  1:2119619/57‑1 (MQ=255)
                          gTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCt                                          <  1:1940781/71‑1 (MQ=255)
                            gAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTAt                                                              >  1:1451398/1‑53 (MQ=255)
                               gTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTcc‑‑cc                                    <  1:843942/70‑1 (MQ=255)
                                 tGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGGCGCGTCt                                                                 >  1:2197221/1‑45 (MQ=255)
                                 tGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCt                                                                 >  1:1664298/1‑45 (MQ=255)
                                 tGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGt                                  >  1:2447871/1‑70 (MQ=255)
                                    tGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGc                              <  1:2023096/71‑1 (MQ=255)
                                    tGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGc                              >  1:3144410/1‑71 (MQ=255)
                                        tGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGa                          >  1:3473915/1‑71 (MQ=255)
                                            cgcgCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAg                      <  1:468940/71‑1 (MQ=255)
                                            cgcgCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAg                      <  1:3733454/71‑1 (MQ=255)
                                              cgcCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGc                    <  1:150269/71‑1 (MQ=255)
                                                ccAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGt                  >  1:5322616/1‑71 (MQ=255)
                                                ccAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGt                  >  1:1534616/1‑71 (MQ=255)
                                                 cAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGtt                 <  1:1838129/71‑1 (MQ=255)
                                                 cAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGtt                 <  1:647468/71‑1 (MQ=255)
                                                  aaTGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGtt                 >  1:125423/1‑70 (MQ=255)
                                                      ccTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGt                                  >  1:5569488/1‑49 (MQ=255)
                                                        tGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCg                                   <  1:2097987/46‑1 (MQ=255)
                                                          aTGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCa        >  1:2453231/1‑71 (MQ=255)
                                                          aTGCGACGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAcg             <  1:5374231/71‑1 (MQ=0)
                                                          aTGCGACGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAcg             >  1:4890722/1‑71 (MQ=0)
                                                          aTGCGACGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAcg             >  1:4963371/1‑71 (MQ=0)
                                                          aTGCGACGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAc              <  1:4719260/70‑1 (MQ=0)
                                                           tGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCg                                   >  1:37603/1‑43 (MQ=255)
                                                           tGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCg                                   >  1:1700515/1‑43 (MQ=255)
                                                           tGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCa        <  1:4427823/70‑1 (MQ=255)
                                                           tGCGACGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTAcgc            >  1:1345902/1‑71 (MQ=0)
                                                            gCGACGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCATCCGTAGGCCGGATAAGGCGTTTAcgcc           >  1:29656/1‑71 (MQ=0)
                                                            gCGACGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCATCCGTAGGCCGGATAAGGCGTTTAcgcc           >  1:4485858/1‑71 (MQ=0)
                                                             cGACGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCATCCGTAGGCCGGATAAGGCGTTTAcgcc           <  1:4567717/70‑1 (MQ=0)
                                                               aCGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCgg   >  1:1858644/1‑71 (MQ=255)
                                                               aCGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCa        <  1:3533806/71‑1 (MQ=0)
                                                               aCGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCa        >  1:1204526/1‑71 (MQ=0)
                                                               aCGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCa        >  1:3608400/1‑71 (MQ=0)
                                                                cGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCAt       <  1:5289091/71‑1 (MQ=0)
                                                                cGCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCCTCCGTAGGCCGGATAAGGCGTTTACGCCGCAt       <  1:4720923/71‑1 (MQ=0)
                                                                 gCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGc  >  1:3375370/1‑70 (MQ=255)
                                                                 gCTTGCGCGTCTTATCAGGCCTACAAATTCCCGCACCATCCGTAGGCCGGATAAGGCGTTTACGCCGCATc      <  1:445947/71‑1 (MQ=0)
                                                                      |                                                                      
AACTATATGCTGCACACGCAGAATAAGTGAGGTTGATGTTTGGCCGCGCCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACAAAT‑‑‑‑GCTCC‑‑CCGTAGGCCGGATAAGGCGTTTACGCCGCATCCGGC  >  Exported/1467812‑1467946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: