BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,693	Δ1 bp	10.6%	coding (1561/2283 nt)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,693	0	G	.	10.6%	287.1 / 24.8	94	coding (1561/2283 nt)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base G (47/36); new base . (4/6); total (51/43)
Fisher's exact test for biased strand distribution p-value = 3.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.58e-01

TTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTG  >  Exported/608623‑608759

ttCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCg                                                                     >  1:4107728/1‑71 (MQ=255)

 gggCATATTTGATGGCAGACAGGGAGTCCGTCGCCACCGACAGGCCCGCGATGCCGCATGCCATAGTGCga                                                                    <  1:373464/69‑2 (MQ=255)

   gCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGAt                                                                  <  1:937516/71‑1 (MQ=255)

    cttatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                                 <  1:2431491/69‑1 (MQ=255)

    cATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                                 <  1:920176/71‑1 (MQ=255)

       atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                             >  1:1149481/1‑71 (MQ=255)

        tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                            >  1:4624953/1‑71 (MQ=255)

        tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                            >  1:3464094/1‑71 (MQ=255)

        tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                            >  1:4120457/1‑71 (MQ=255)

         ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                            >  1:4482926/1‑70 (MQ=255)

         ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                            >  1:3549713/1‑70 (MQ=255)

          tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTc                                                           <  1:4495706/70‑1 (MQ=255)

          tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACACCATGGTGCGGATA‑ACGTCa                                                          >  1:1826472/1‑71 (MQ=255)

             ttGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                                       <  1:709857/71‑1 (MQ=255)

              tGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                                      >  1:3881570/1‑71 (MQ=255)

               gCAGACAGGGAGTCAGCAGCGACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                     >  1:1409918/1‑71 (MQ=255)

               gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                               >  1:5666001/1‑61 (MQ=255)

               gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                               >  1:2960014/1‑61 (MQ=255)

               gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                     >  1:4431275/1‑71 (MQ=255)

               gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                     >  1:5365467/1‑71 (MQ=255)

               gCAGACAGGGAGTCAGCAGCAACGGACAGAACAGCGATACCACACGCCATGGTGCGGATa‑a                                                               >  1:898897/1‑61 (MQ=255)

                cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                     <  1:70206/70‑1 (MQ=255)

                cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCg                                                    >  1:3893114/1‑71 (MQ=255)

                  gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCgcg                                                  <  1:994016/71‑3 (MQ=255)

                  gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                  <  1:2664318/71‑1 (MQ=255)

                   aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                  >  1:4380746/1‑70 (MQ=255)

                   aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGc                                                 <  1:259841/71‑1 (MQ=255)

                    cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGc                                                 <  1:5063168/70‑1 (MQ=255)

                    cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                <  1:2870297/71‑1 (MQ=255)

                     aGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAg                                               >  1:4929491/1‑71 (MQ=255)

                       ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                >  1:4325930/1‑68 (MQ=255)

                       ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                >  1:767944/1‑68 (MQ=255)

                           tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                         <  1:5488704/71‑1 (MQ=255)

                             agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAg                                               >  1:4966893/1‑63 (MQ=255)

                             agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                         >  1:2339797/1‑69 (MQ=255)

                             agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                         >  1:3859894/1‑69 (MQ=255)

                             agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                       <  1:5218487/71‑1 (MQ=255)

                                  caACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                              <  1:1283199/43‑1 (MQ=255)

                                   aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                              <  1:1612146/42‑1 (MQ=255)

                                    aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga                                   >  1:606469/1‑68 (MQ=255)

                                    aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga                                   >  1:5597367/1‑68 (MQ=255)

                                    aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGc                                >  1:225641/1‑71 (MQ=255)

                                     ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                               >  1:2536424/3‑71 (MQ=255)

                                      ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                              <  1:203083/71‑1 (MQ=255)

                                        aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                            >  1:190803/1‑71 (MQ=255)

                                              ccgCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTg                      >  1:2590246/3‑71 (MQ=255)

                                              cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                              >  1:449662/1‑63 (MQ=255)

                                              cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                              >  1:4797998/1‑63 (MQ=255)

                                              cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                              >  1:789345/1‑63 (MQ=255)

                                              cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                              >  1:4804471/1‑63 (MQ=255)

                                              cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                      <  1:4899071/71‑1 (MQ=255)

                                              cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGGCGTGCAGCGCCAt                                         <  1:4551539/52‑1 (MQ=255)

                                                gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                         <  1:1076884/50‑1 (MQ=255)

                                                gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                         <  1:5505039/50‑1 (MQ=255)

                                                gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                         <  1:774347/50‑1 (MQ=255)

                                                gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                         <  1:4739138/50‑1 (MQ=255)

                                                gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                    >  1:1757715/1‑71 (MQ=255)

                                                 cGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                         <  1:3946373/49‑1 (MQ=255)

                                                  gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                  >  1:5614179/1‑39 (MQ=255)

                                                  gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                     >  1:2844041/1‑52 (MQ=255)

                                                  gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                       >  1:761863/1‑50 (MQ=255)

                                                  gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTAc                   >  1:4218048/1‑70 (MQ=255)

                                                  gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACt                  >  1:4981778/1‑71 (MQ=255)

                                                   aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                             >  1:2933757/1‑59 (MQ=255)

                                                    tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgc                                              >  1:350219/1‑41 (MQ=255)

                                                      ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                    >  1:2049538/1‑49 (MQ=255)

                                                      ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                    >  1:1411553/1‑49 (MQ=255)

                                                      ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                      <  1:4109552/63‑1 (MQ=255)

                                                      ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                      <  1:1775790/63‑1 (MQ=255)

                                                      ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt               <  1:4063157/70‑1 (MQ=255)

                                                      ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt               <  1:1880320/70‑1 (MQ=255)

                                                      ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTc              <  1:4524482/71‑1 (MQ=255)

                                                       cacaCGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt               <  1:3882643/69‑1 (MQ=255)

                                                       cacaCGCCATCGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                     >  1:4626461/1‑47 (MQ=255)

                                                        acacGCCATGGTGCTGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt            <  1:5588762/71‑1 (MQ=255)

                                                        acacGCCATGGTGCGGATG‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt            <  1:5425087/71‑1 (MQ=255)

                                                        acacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                    >  1:5594075/1‑47 (MQ=255)

                                                        acacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                    >  1:2945116/1‑47 (MQ=255)

                                                         catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTg           <  1:4083297/68‑1 (MQ=255)

                                                         catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTg           <  1:3102444/68‑1 (MQ=255)

                                                         catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTg           <  1:3557553/68‑1 (MQ=255)

                                                         cacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTg           >  1:3314966/1‑71 (MQ=255)

                                                          acGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGc          <  1:3358513/71‑1 (MQ=255)

                                                           tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATc                                        <  1:126045/39‑1 (MQ=9)

                                                           tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATc                                        <  1:965006/39‑1 (MQ=9)

                                                           tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATc                                        <  1:1138975/39‑1 (MQ=9)

                                                           tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa         >  1:568748/2‑71 (MQ=255)

                                                           tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa         >  1:3838314/2‑71 (MQ=255)

                                                             ccATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg       <  1:4580196/71‑1 (MQ=255)

                                                              cATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGt      <  1:4603465/71‑1 (MQ=255)

                                                               aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTg           <  1:3682641/65‑1 (MQ=255)

                                                               aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTg           <  1:5161873/65‑1 (MQ=255)

                                                                  gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                               >  1:4085135/1‑42 (MQ=255)

                                                                  gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                             >  1:4631848/1‑44 (MQ=255)

                                                                  gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTg  <  1:1283842/71‑1 (MQ=255)

                                                                   tGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga                                   >  1:3987840/1‑37 (MQ=255)

                                                                      ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAg    >  1:697820/1‑65 (MQ=255)

TTCGCATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTG  >  Exported/608623‑608759

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`