| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,002,425:1 | +G | 9.1% | intergenic (+72/+51) | yghU → / ← hybG | predicted S‑transferase/hydrogenase 2 accessory protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,002,425 | 1 | . | G | 9.1% | 295.2 / 28.6 | 77 | intergenic (+72/+51) | yghU/hybG | predicted S‑transferase/hydrogenase 2 accessory protein |
| Reads supporting (aligned to +/- strand): ref base . (41/29); new base G (3/4); total (44/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.54e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.47e-01 | |||||||||||
GTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCG > Exported/2002356‑2002494 | gTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACa‑a > 1:990705/1‑71 (MQ=255)gTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACa‑a < 1:4667310/71‑1 (MQ=255) gtgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGc < 1:914092/70‑1 (MQ=255) gtgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGc < 1:3137013/70‑1 (MQ=255) tgtTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑Ataga > 1:4184107/1‑67 (MQ=255) ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTg < 1:1579360/70‑1 (MQ=255) ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGa < 1:3091002/71‑1 (MQ=255) ttCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGa < 1:2235435/71‑1 (MQ=255) tcgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCATGCCGCATCCGGCAATCGTGCACA‑ATGCCTGAt > 1:2414298/1‑71 (MQ=255) tcgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGAt > 1:3347479/1‑71 (MQ=255) cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATg > 1:3395797/1‑71 (MQ=255) cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATg > 1:800646/1‑71 (MQ=255) cgtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATg > 1:3300464/1‑71 (MQ=255) gtcgCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGc < 1:2251105/71‑1 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGAc > 1:2601440/1‑71 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGAc > 1:4786456/1‑71 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGAc < 1:3554549/71‑1 (MQ=255) gcagcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGAc > 1:3892702/1‑71 (MQ=255) gcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑t > 1:2718058/1‑71 (MQ=255) gcaAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑t > 1:1706087/1‑71 (MQ=255) caAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑Tg > 1:549561/1‑71 (MQ=255) caAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑Tg > 1:3460609/1‑71 (MQ=255) aGCCATCCAGGCCGGATAAGGCGTTCCCGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGcc > 1:300041/1‑71 (MQ=255) aGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGc > 1:5213317/1‑70 (MQ=255) cATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCcgcg > 1:3412365/1‑71 (MQ=255) taGGCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCt > 1:1747449/2‑71 (MQ=11) cAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCt > 1:547717/1‑70 (MQ=255) aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTa < 1:2424158/71‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCtt < 1:2369869/70‑1 (MQ=1) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTa > 1:5575257/1‑71 (MQ=1) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTAt < 1:398235/71‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTAt < 1:1976584/71‑1 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTAt < 1:366548/71‑1 (MQ=1) ccGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCa < 1:2557997/71‑1 (MQ=255) gATAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAgg > 1:103501/1‑71 (MQ=1) aTAAGGCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAgac < 1:2140569/71‑3 (MQ=1) aTAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGcc > 1:299414/1‑71 (MQ=255) aaGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGc > 1:5086911/1‑50 (MQ=255) aaGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTa > 1:502717/1‑71 (MQ=255) aGGCGTTCACGCCGCATCCGGCAGTCGTGCGCC‑ATGCCTGATGCGACGCTTTGCGCGTCTTATCAGGCCt < 1:143691/70‑1 (MQ=11) aGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCt < 1:4704908/39‑1 (MQ=255) ggCGTTCACGCCGCATCCGGCAGTCGTGCGCAGATGCCTGATGCGACGC‑TAACGCGTCTTATCAGGCCTAc < 1:4056090/71‑1 (MQ=1) ggCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGc < 1:4087008/43‑1 (MQ=255) gCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGa < 1:5425052/71‑1 (MQ=255) cGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaa < 1:4972006/71‑1 (MQ=255) cGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaa < 1:2040050/71‑1 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:5073097/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:445070/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:2566937/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:2818161/1‑71 (MQ=255) tCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:1811793/1‑71 (MQ=255) cACGCCGCATCCGGCAATCGTTCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc < 1:2863666/70‑1 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGa > 1:448828/1‑66 (MQ=255) cACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGc > 1:1488499/1‑70 (MQ=255) aCGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCt < 1:270106/70‑1 (MQ=255) aCGCCGCATCCGGCAATCGAGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTa < 1:3990030/71‑1 (MQ=255) ccgcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTaa > 1:3214259/1‑69 (MQ=255) ccgcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatc < 1:5619053/71‑1 (MQ=255) gcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat < 1:1862813/71‑1 (MQ=255) gcATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat < 1:1283253/71‑1 (MQ=255) ccGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcg > 1:853808/1‑71 (MQ=255) cGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTaa > 1:1066588/1‑62 (MQ=255) cGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc > 1:3899269/1‑71 (MQ=255) cGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgc > 1:2264226/1‑71 (MQ=255) gCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACgcgc > 1:313665/1‑71 (MQ=255) cAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCt > 1:4051214/1‑71 (MQ=255) cAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCt > 1:3221371/1‑71 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGaa < 1:4997144/52‑1 (MQ=255) aaTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg > 1:4606671/1‑71 (MQ=255) aTCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg < 1:1021144/71‑1 (MQ=255) tCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatc > 1:5196056/1‑58 (MQ=255) tCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTgg > 1:4487556/1‑70 (MQ=255) tCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGt < 1:5235391/71‑1 (MQ=255) cGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatcat > 1:4082724/1‑59 (MQ=255) cGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCAcgcg > 1:3916511/1‑65 (MQ=255) cGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGt < 1:5005017/70‑1 (MQ=255) gTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAatca < 1:307170/57‑1 (MQ=255) gTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTaa > 1:2209611/1‑71 (MQ=255) tGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAAt < 1:3547729/71‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATg < 1:2037908/70‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATg < 1:3390203/70‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATg < 1:3538600/70‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt < 1:2162253/71‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt < 1:887715/71‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt < 1:3471460/71‑1 (MQ=255) caca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt > 1:4828888/1‑71 (MQ=255) aca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGt < 1:5081828/70‑1 (MQ=255) ca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACgcgc < 1:1787944/60‑1 (MQ=255) ca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTg > 1:4726473/1‑62 (MQ=255) ca‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCg < 1:615478/71‑1 (MQ=255) a‑aTGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCg > 1:3729234/1‑70 (MQ=255) | GTTGGTGTTCGTCGCAGCAAGCCATCCAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGTGCACA‑ATGCCTGATGCGACGC‑TGCCGCGTCTTATCAGGCCTACGAAAGCTAATCATCACGCGCTGGTAATGTCG > Exported/2002356‑2002494 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |