| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 2,773,680:1 | +C | 15.9% | intergenic (+69/‑114) | aceA → / → aceK | isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 2,773,680 | 1 | . | C | 15.9% | 200.7 / 51.0 | 69 | intergenic (+69/‑114) | aceA/aceK | isocitrate lyase/isocitrate dehydrogenase kinase/phosphatase |
| Reads supporting (aligned to +/- strand): ref base . (32/26); new base C (4/7); total (36/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.30e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.16e-01 | |||||||||||
GCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTT > Exported/2773612‑2773750 | gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:2750278/1‑71 (MQ=255)gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:4473628/1‑71 (MQ=255)gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:4513233/1‑71 (MQ=255)gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:1488604/1‑71 (MQ=255)gCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑Tg > 1:2082521/1‑71 (MQ=255) acaacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCt < 1:551396/71‑1 (MQ=255) caacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCt > 1:5141301/1‑70 (MQ=255) caacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTg < 1:1795518/71‑1 (MQ=255) aacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGa > 1:3714733/1‑71 (MQ=255) aacaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGa > 1:2894040/1‑71 (MQ=255) acaacCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGAt > 1:499976/1‑71 (MQ=255) gACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTgcg > 1:5337156/1‑71 (MQ=255) cTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGcgcg > 1:4337777/1‑71 (MQ=255) cTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGcgcg < 1:310363/71‑1 (MQ=255) aGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCt > 1:4861730/1‑70 (MQ=255) ggCCGGATAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTa < 1:2980922/70‑1 (MQ=11) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGc < 1:1754404/54‑1 (MQ=255) ggCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCtt < 1:702953/70‑1 (MQ=255) gaccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCtt < 1:3613819/68‑1 (MQ=1) gaccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTa < 1:3007464/69‑1 (MQ=11) gaccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTa < 1:3307294/69‑1 (MQ=11) gaccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTa < 1:895741/69‑1 (MQ=11) agCCGGATAAGGCGCTCGCGCCGCATCCGGCAA‑CGGTGCCAGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:1428683/70‑1 (MQ=14) gCCGGATAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:4359086/70‑1 (MQ=11) gCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:2817935/71‑1 (MQ=255) gCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:1913176/71‑1 (MQ=255) accGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTAt < 1:1166876/70‑1 (MQ=11) ccGGATAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCa < 1:3793475/71‑1 (MQ=11) ccGGATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTATc < 1:4271156/71‑1 (MQ=11) cGGATAAGGCGCTCGCGCCGCATCCGGCAA‑CGGTGCCAGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCag > 1:1015364/1‑70 (MQ=14) ggATAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:5187437/67‑1 (MQ=11) ggATAAGGCGTTCACGCCGCATCCGGCAGTC‑GTGCGCCA‑TGCCTGATGCGACGCTTTGCGCGTCTTATCag > 1:455277/1‑70 (MQ=1) aTAAGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGcc > 1:3814546/1‑71 (MQ=11) aaGGCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGCCTa > 1:234268/1‑71 (MQ=11) aGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCt < 1:3737784/70‑1 (MQ=255) ggCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGCCTa > 1:3284257/1‑69 (MQ=11) ggCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGCCTACa < 1:169435/71‑1 (MQ=11) ggCGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTATCAGGCCTACa < 1:3713134/71‑1 (MQ=11) ggCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTAc < 1:423264/71‑1 (MQ=255) cGTTTACGCCGCATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTCTTAt > 1:4935700/1‑59 (MQ=11) ttCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGc < 1:214201/40‑1 (MQ=40) ttCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGcc < 1:5093041/71‑1 (MQ=255) ttCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGcc > 1:4729389/1‑71 (MQ=255) aCGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGtt > 1:27977/1‑71 (MQ=255) aCGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGtt > 1:4200124/1‑71 (MQ=255) cgccgcATCCGGCAA‑CGGTGC‑CGACTGCCTGATGCGACGC‑TTGCGCGTc < 1:4656924/49‑1 (MQ=11) cGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGt < 1:2852359/70‑1 (MQ=255) gCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAg < 1:1052830/70‑1 (MQ=255) cGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTAt < 1:2871819/38‑1 (MQ=255) cGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:1168418/1‑71 (MQ=255) cGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:2656109/1‑71 (MQ=255) cGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:423878/1‑71 (MQ=255) ggTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:1673876/1‑70 (MQ=255) ggTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt < 1:604840/71‑1 (MQ=255) ggTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt < 1:2272111/71‑1 (MQ=255) ggTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt < 1:1384998/71‑1 (MQ=255) tGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt > 1:5650249/1‑69 (MQ=255) gCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt > 1:1162796/1‑68 (MQ=255) gCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt > 1:4762110/1‑68 (MQ=255) gCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGAt > 1:2142608/1‑68 (MQ=255) cACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:4325146/1‑66 (MQ=255) cACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:4695927/1‑66 (MQ=255) cACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGa > 1:5216082/1‑66 (MQ=255) cACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATa > 1:2144495/1‑68 (MQ=255) cACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTAAAGCCGTTGCCGAACGTAGGCTGGa > 1:3206706/1‑66 (MQ=255) aCGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGc < 1:272331/71‑1 (MQ=255) cGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCg > 1:2472219/1‑71 (MQ=255) gA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGt > 1:2886272/1‑71 (MQ=255) gA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGt < 1:5626522/71‑1 (MQ=255) a‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCg < 1:2427619/49‑1 (MQ=255) a‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGtt < 1:322042/71‑1 (MQ=255) a‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGtt < 1:1874956/71‑1 (MQ=255) | GCAACAACAACCGTTGCTGACTGTAGGCCGGATAAGGCGTTCACGCCGCATCCGGCAATCGGTGCACGA‑TGCCTGATGCGACGC‑TTGCGCGTCTTATCATGCCTACAGCCGTTGCCGAACGTAGGCTGGATAAGGCGTT > Exported/2773612‑2773750 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |