| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 464,884:1 | +T | 24.1% | intergenic (+24/‑277) | sdhB → / → sucA | succinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamin‑requiring |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 464,884 | 1 | . | T | 24.1% | 122.0 / 65.4 | 58 | intergenic (+24/‑277) | sdhB/sucA | succinate dehydrogenase, FeS subunit/2‑oxoglutarate decarboxylase, thiamin‑requiring |
| Reads supporting (aligned to +/- strand): ref base . (31/13); new base T (9/5); total (40/18) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.44e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGT > Exported/464814‑464954 | gcgcCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCa < 1:5465554/71‑1 (MQ=255) gcCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑g > 1:624876/1‑71 (MQ=255) aTCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGt > 1:1090347/1‑71 (MQ=255) aTCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGt > 1:35157/1‑71 (MQ=255) ggCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCg > 1:4829995/1‑70 (MQ=255) ggCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGc < 1:1246320/71‑1 (MQ=255) atatCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCa < 1:3049843/71‑1 (MQ=255) tatCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAg > 1:5031890/1‑71 (MQ=255) tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCa‑a > 1:1826767/1‑57 (MQ=255) tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCa‑a > 1:324804/1‑57 (MQ=255) tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑g > 1:367953/1‑58 (MQ=255) tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑g > 1:5551587/1‑58 (MQ=255) tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑g > 1:440743/1‑58 (MQ=255) tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑g > 1:3356972/1‑58 (MQ=255) tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCAt > 1:4153199/1‑66 (MQ=255) tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGc < 1:5116807/71‑1 (MQ=255) tCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGc < 1:4598662/71‑1 (MQ=255) cAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGc > 1:2353200/1‑70 (MQ=255) aGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAAc > 1:1794064/1‑71 (MQ=255) aGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAAc > 1:799971/1‑71 (MQ=255) gATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAg > 1:4378387/1‑70 (MQ=255) aTGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAAc > 1:2290297/1‑66 (MQ=255) aTGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAAc > 1:5328296/1‑66 (MQ=255) aTGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAAc > 1:1624072/1‑66 (MQ=255) tgttgtTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGc > 1:5400891/1‑71 (MQ=255) gttgttGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGcc > 1:2855310/1‑71 (MQ=255) ttgttgCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTg < 1:1834866/68‑1 (MQ=255) gttgCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCg > 1:1339669/1‑50 (MQ=255) gttgCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCg > 1:777925/1‑50 (MQ=255) tacacttcgcccaTAAACCGTAGGCCTGATAAGACGCGCa < 1:192668‑M1/27‑1 (MQ=255) tacacttcgcccaTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTc > 1:1920950‑M1/14‑47 (MQ=255) tacacttcgcccaTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTc > 1:4022236‑M1/14‑47 (MQ=255) tacacttcgcccaTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTc > 1:373375‑M1/14‑47 (MQ=255) tacacttcgcccaTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTc > 1:5000250‑M1/14‑47 (MQ=255) gCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGc > 1:2531986/1‑48 (MQ=255) gCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGc > 1:3950061/1‑48 (MQ=255) gCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGc > 1:1913771/1‑48 (MQ=255) gCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGc > 1:1694434/1‑48 (MQ=255) cacttcgcccgTAAGCCGTAGGCCTGATAAGACGCGCA‑AAGCGTCGCAt < 1:3447958‑M1/38‑1 (MQ=255) cacttcgcccaTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTCGc < 1:5248528‑M1/36‑1 (MQ=255) cacttcgcccaTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTCGc < 1:2213374‑M1/36‑1 (MQ=255) cacttcgcccaTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTCGc < 1:5409598‑M1/36‑1 (MQ=255) cacttcgcccaTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTCGCAt < 1:4947273‑M1/38‑1 (MQ=255) cttcgcccaTAAACCGTAGGACTGATAAGACGCGCATA‑GCGTCGCATCAGGCa > 1:2507995‑M1/10‑53 (MQ=255) tcgcccaTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTCGCAt < 1:309600‑M1/38‑1 (MQ=255) tAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTg < 1:3840306/71‑1 (MQ=255) tAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTg < 1:3085767/71‑1 (MQ=255) cgcccgTAAGCCGTAGGCCTGATAAGACGCGCA‑AAGCGTCGCAt < 1:276795‑M1/38‑1 (MQ=255) aTGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGaa < 1:2752619/71‑1 (MQ=255) aTGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGaa > 1:4074436/1‑71 (MQ=255) aTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTCGc > 1:98135‑M1/2‑37 (MQ=255) aTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTCGc > 1:2218124‑M1/2‑37 (MQ=255) aTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTCGc > 1:951124‑M1/2‑37 (MQ=255) aTAAACCGTAGGCCTGATAAGACGCGCATA‑GCGTCGc > 1:3048109‑M1/2‑37 (MQ=255) ccGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATc < 1:2056247/71‑1 (MQ=255) aTAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCGTTATCCGGCCTACAAg > 1:5401865/1‑71 (MQ=255) aTAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACa < 1:4838075/69‑1 (MQ=255) aTAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAg > 1:4453097/1‑71 (MQ=255) aTAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAg > 1:351885/1‑71 (MQ=255) gACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCAt < 1:4442509/71‑1 (MQ=255) aCGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCAt > 1:1394081/1‑70 (MQ=255) aCGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCAt > 1:884615/1‑70 (MQ=255) a‑a‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCgg > 1:4186831/1‑52 (MQ=255) a‑a‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGt < 1:3415362/71‑1 (MQ=255) | GCGCCATCGGCCATATCAAGTCGATGTTGTTGCAACGTAATGCGTAAACCGTAGGCCTGATAAGACGCGCA‑A‑GCGTCGCATCAGGCAACCAGTGCCGGATGCGGCGTGAACGCCTTATCCGGCCTACAAGTCATTACCCGT > Exported/464814‑464954 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |