BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	608,697:1	+G	10.8%	coding (1557/2283 nt)	pflB ←	pyruvate formate lyase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	608,697	1	.	G	10.8%	310.7 / 43.5	93	coding (1557/2283 nt)	pflB	pyruvate formate lyase I
Reads supporting (aligned to +/- strand): ref base . (49/34); new base G (4/6); total (53/40)
Fisher's exact test for biased strand distribution p-value = 3.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 101 total aligned reads displayed.

CATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGT  >  Exported/608627‑608767

cttatTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                                         <  1:2431491/69‑1 (MQ=255)

cATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa                                                                         <  1:920176/71‑1 (MQ=255)

   atTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACg                                                                     >  1:1149481/1‑71 (MQ=255)

    tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                    >  1:4624953/1‑71 (MQ=255)

    tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                    >  1:4120457/1‑71 (MQ=255)

    tttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                    >  1:3464094/1‑71 (MQ=255)

     ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                    >  1:4482926/1‑70 (MQ=255)

     ttGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGt                                                                    >  1:3549713/1‑70 (MQ=255)

      tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTc                                                                   <  1:4495706/70‑1 (MQ=255)

      tGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACACCATGGTGCGGATA‑ACGTCa                                                                  >  1:1826472/1‑71 (MQ=255)

         ttGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACgg                                                               <  1:709857/71‑1 (MQ=255)

          tGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGt                                                              >  1:3881570/1‑71 (MQ=255)

           gCAGACAGGGAGTCAGCAGCGACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                             >  1:1409918/1‑71 (MQ=255)

           gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                                       >  1:2960014/1‑61 (MQ=255)

           gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATa‑a                                                                       >  1:5666001/1‑61 (MQ=255)

           gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                             >  1:4431275/1‑71 (MQ=255)

           gCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                             >  1:5365467/1‑71 (MQ=255)

           gCAGACAGGGAGTCAGCAGCAACGGACAGAACAGCGATACCACACGCCATGGTGCGGATa‑a                                                                       >  1:898897/1‑61 (MQ=255)

            cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTc                                                             <  1:70206/70‑1 (MQ=255)

            cAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCg                                                            >  1:3893114/1‑71 (MQ=255)

              gACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                          <  1:2664318/71‑1 (MQ=255)

               aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                          >  1:4380746/1‑70 (MQ=255)

               aCAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGc                                                         <  1:259841/71‑1 (MQ=255)

                cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGc                                                         <  1:5063168/70‑1 (MQ=255)

                cAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                        <  1:2870297/71‑1 (MQ=255)

                 aGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAg                                                       >  1:4929491/1‑71 (MQ=255)

                   ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                        >  1:4325930/1‑68 (MQ=255)

                   ggAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCa                                                        >  1:767944/1‑68 (MQ=255)

                       tCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 <  1:5488704/71‑1 (MQ=255)

                         agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAg                                                       >  1:4966893/1‑63 (MQ=255)

                         agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 >  1:2339797/1‑69 (MQ=255)

                         agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 >  1:3859894/1‑69 (MQ=255)

                         agcagcAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                               <  1:5218487/71‑1 (MQ=255)

                              caACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                                      <  1:1283199/43‑1 (MQ=255)

                               aaCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑Ac                                                                      <  1:1612146/42‑1 (MQ=255)

                                aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga                                           >  1:5597367/1‑68 (MQ=255)

                                aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga                                           >  1:606469/1‑68 (MQ=255)

                                aCGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGc                                        >  1:225641/1‑71 (MQ=255)

                                 ccgACAGGCCCGCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCt                                       >  1:2536424/3‑71 (MQ=255)

                                  ggACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                      <  1:203083/71‑1 (MQ=255)

                                    aCAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                    >  1:190803/1‑71 (MQ=255)

                                          ccgCGATGCCGCATGCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTg                              >  1:2590246/3‑71 (MQ=255)

                                          cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                      >  1:4797998/1‑63 (MQ=255)

                                          cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                      >  1:4804471/1‑63 (MQ=255)

                                          cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                      >  1:449662/1‑63 (MQ=255)

                                          cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCtt                                      >  1:789345/1‑63 (MQ=255)

                                          cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                              <  1:4899071/71‑1 (MQ=255)

                                          cAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGGCGTGCAGCGCCAt                                                 <  1:4551539/52‑1 (MQ=255)

                                            gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 <  1:4739138/50‑1 (MQ=255)

                                            gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 <  1:5505039/50‑1 (MQ=255)

                                            gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 <  1:774347/50‑1 (MQ=255)

                                            gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 <  1:1076884/50‑1 (MQ=255)

                                            gCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTa                            >  1:1757715/1‑71 (MQ=255)

                                             cGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCAt                                                 <  1:3946373/49‑1 (MQ=255)

                                              gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTg                                                          >  1:5614179/1‑39 (MQ=255)

                                              gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                             >  1:2844041/1‑52 (MQ=255)

                                              gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCa                                               >  1:761863/1‑50 (MQ=255)

                                              gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTAc                           >  1:4218048/1‑70 (MQ=255)

                                              gATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACt                          >  1:4981778/1‑71 (MQ=255)

                                               aTACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                                     >  1:2933757/1‑59 (MQ=255)

                                                tACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAgc                                                      >  1:350219/1‑41 (MQ=255)

                                                  ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                            >  1:2049538/1‑49 (MQ=255)

                                                  ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                            >  1:1411553/1‑49 (MQ=255)

                                                  ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                              <  1:1775790/63‑1 (MQ=255)

                                                  ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTg                              <  1:4109552/63‑1 (MQ=255)

                                                  ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                       <  1:1880320/70‑1 (MQ=255)

                                                  ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                       <  1:4063157/70‑1 (MQ=255)

                                                  ccACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTc                      <  1:4524482/71‑1 (MQ=255)

                                                   cacaCGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGt                       <  1:3882643/69‑1 (MQ=255)

                                                   cacaCGCCATCGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCaga                                             >  1:4626461/1‑47 (MQ=255)

                                                    acacGCCATGGTGCTGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                    <  1:5588762/71‑1 (MQ=255)

                                                    acacGCCATGGTGCGGATG‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGt                    <  1:5425087/71‑1 (MQ=255)

                                                    acacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                            >  1:2945116/1‑47 (MQ=255)

                                                    acacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagag                                            >  1:5594075/1‑47 (MQ=255)

                                                     catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTg                   <  1:3102444/68‑1 (MQ=255)

                                                     catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTg                   <  1:3557553/68‑1 (MQ=255)

                                                     catgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTg                   <  1:4083297/68‑1 (MQ=255)

                                                     cacGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTg                   >  1:3314966/1‑71 (MQ=255)

                                                      acGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGc                  <  1:3358513/71‑1 (MQ=255)

                                                       tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATc                                                <  1:965006/39‑1 (MQ=9)

                                                       tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATc                                                <  1:1138975/39‑1 (MQ=9)

                                                       tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATc                                                <  1:126045/39‑1 (MQ=9)

                                                       tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa                 >  1:568748/2‑71 (MQ=255)

                                                       tgCCATAGTGC‑GATAGACATCACGATCGTGCAGCGCCATCAGCGAAGCTTCGTAGCTGTACTTGTCGTGCa                 >  1:3838314/2‑71 (MQ=255)

                                                         ccATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATg               <  1:4580196/71‑1 (MQ=255)

                                                          cATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGt              <  1:4603465/71‑1 (MQ=255)

                                                           aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTg                   <  1:5161873/65‑1 (MQ=255)

                                                           aTGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTg                   <  1:3682641/65‑1 (MQ=255)

                                                              gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCt                                       >  1:4085135/1‑42 (MQ=255)

                                                              gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTc                                     >  1:4631848/1‑44 (MQ=255)

                                                              gTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTg          <  1:1283842/71‑1 (MQ=255)

                                                               tGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCagaga                                           >  1:3987840/1‑37 (MQ=255)

                                                                  ggATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAg            >  1:697820/1‑65 (MQ=255)

                                                                    aTA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGgatga     >  1:1649975/1‑70 (MQ=255)

                                                                     tA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                    >  1:1718547/1‑38 (MQ=255)

                                                                     tA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCg                                    >  1:3158117/1‑38 (MQ=255)

                                                                     tA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGatgatg   >  1:2485028/1‑71 (MQ=255)

                                                                      a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGTCTTc                                     >  1:1685677/1‑36 (MQ=255)

                                                                      a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGt  >  1:3993825/1‑71 (MQ=255)

                                                                      a‑aCGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGt  >  1:5158606/1‑71 (MQ=255)

CATATTTGATTGCAGACAGGGAGTCAGCAGCAACGGACAGACCAGCGATACCACACGCCATGGTGCGGATA‑ACGTCACGGTCGTGCAGCGCCATCAGAGAGGCTTCGTAGCTGTACTTGTCGTGCATGTAGTGGATGATGT  >  Exported/608627‑608767

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`