Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 821,537:1 +T 19.6% intergenic (‑74/+380) ldrA ← / ← ldrC toxic polypeptide, small/toxic polypeptide, small

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported821,5371.T19.6% 137.2 / 44.1 51intergenic (‑74/+380)ldrA/ldrCtoxic polypeptide, small/toxic polypeptide, small
Reads supporting (aligned to +/- strand):  ref base . (16/25);  new base T (4/6);  total (20/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01

GCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCT  >  Exported/821493‑821604
                                             |                                                                   
gccgccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAAATTAGCCCCCGTTc                                           <  1:3016986/71‑1 (MQ=14)
  cgccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCtg                                         <  1:4219937/71‑1 (MQ=34)
    ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCtgtt                                       >  1:4893763/1‑71 (MQ=34)
    ccTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCtgt                                        >  1:589954/1‑70 (MQ=35)
     cTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCtgttg                                      <  1:2806379/71‑1 (MQ=34)
     cTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCtgttg                                      <  1:1714718/71‑1 (MQ=34)
       tgttgtAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCtgtt                                       <  1:940135/68‑1 (MQ=35)
        gttgtAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCtgttgt                                     >  1:374624/1‑69 (MQ=35)
           gtAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAgg                                 <  1:3061370/70‑1 (MQ=35)
           gtAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAgg                                 <  1:4660628/70‑1 (MQ=35)
              aTGACAACATTTTGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttgt                             <  1:155652/71‑3 (MQ=11)
              aTGACAACATTTTGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttg                              >  1:3273401/1‑69 (MQ=11)
              aTGACAACATTTTGCGGCTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttg                              >  1:1338530/1‑69 (MQ=11)
              aTGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtttt                             <  1:1909009/71‑1 (MQ=34)
              aTGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt                              >  1:4937720/1‑70 (MQ=35)
              aTGACAACATTTTGCGACTATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttgt                             <  1:3754784/71‑3 (MQ=11)
               tGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAg                                  <  1:2893014/65‑1 (MQ=35)
                   aacaTTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc                        >  1:762428/1‑71 (MQ=34)
                   aacaTTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc                        >  1:1018866/1‑71 (MQ=34)
                   aacaTTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc                        >  1:4271205/1‑71 (MQ=34)
                    acaTTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc                        <  1:5348219/70‑1 (MQ=35)
                     caTTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACctc                        <  1:2749988/69‑1 (MQ=35)
                      aTTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCtctc                      >  1:4164091/1‑70 (MQ=35)
                         ttGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACg                  >  1:1730592/1‑71 (MQ=34)
                            cGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGt                 <  1:753174/69‑1 (MQ=35)
                              gCTATTCTTGAATTGTTCTGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACct                         <  1:1570890/59‑1 (MQ=11)
                              gCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg                <  1:3472529/68‑1 (MQ=35)
                              gCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg                <  1:1553419/68‑1 (MQ=35)
                              gCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg                <  1:2987968/68‑1 (MQ=35)
                              gCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg                <  1:4483300/68‑1 (MQ=35)
                              gCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTg                <  1:1546760/68‑1 (MQ=35)
                               cTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCggg            >  1:1886804/1‑71 (MQ=34)
                               cTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCgg             <  1:175163/70‑1 (MQ=35)
                                tATTCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttgt                             <  1:3070741/53‑3 (MQ=11)
                                  ttCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCtgtt                                       <  1:148253/41‑1 (MQ=9)
                                  ttCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAg                                  <  1:4106768/46‑1 (MQ=11)
                                  ttCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAg                                  <  1:281134/46‑1 (MQ=11)
                                  ttCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGt         >  1:1290788/1‑71 (MQ=11)
                                  ttCTTGAATTGTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGt         >  1:1870206/1‑71 (MQ=11)
                                  ttCTTGAATTGTTCAGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCaa                    <  1:1278158/60‑1 (MQ=11)
                                   tCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCa                                   <  1:3543476/44‑1 (MQ=38)
                                   tCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGtt        >  1:5693301/1‑71 (MQ=34)
                                    cTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc                           >  1:2826786/1‑51 (MQ=35)
                                     ttGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAgg                                 >  1:1922856/1‑44 (MQ=38)
                                     ttGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAgg                                 >  1:1133119/1‑44 (MQ=38)
                                     ttGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCATGTTTTACCTCTCAACGTGCGGGGGtt        <  1:3721880/69‑1 (MQ=25)
                                     ttGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGttt                              <  1:1544248/47‑1 (MQ=38)
                                     ttGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGtt                               >  1:640946/1‑46 (MQ=38)
                                     ttGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa                            <  1:3133947/49‑1 (MQ=38)
                                     ttGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGttt       >  1:756040/1‑70 (MQ=35)
                                         aGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTctct  <  1:459328/71‑1 (MQ=34)
                                          gtgtCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTAc                           >  1:759367/1‑45 (MQ=38)
                                           tgtCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtct    <  1:402836/67‑1 (MQ=35)
                                           tgtCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTtct    <  1:1454530/67‑1 (MQ=35)
                                            gtCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa                            >  1:2406855/1‑42 (MQ=38)
                                            gtCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa                            >  1:3040576/1‑42 (MQ=38)
                                            gtCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa                            >  1:3918708/1‑42 (MQ=38)
                                            gtCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTa                            >  1:3507434/1‑42 (MQ=38)
                                             |                                                                   
GCCGCCTTGTTGTAATGACAACATTTTGCGGCTATTCTTGAAGTGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCTCT  >  Exported/821493‑821604

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: