Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 189,651:1 +C 15.1% coding (1148/2142 nt) ldcC → lysine decarboxylase 2, constitutive

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported189,6511.C15.1% 168.1 / 35.2 53coding (1148/2142 nt)ldcClysine decarboxylase 2, constitutive
Reads supporting (aligned to +/- strand):  ref base . (24/21);  new base C (3/5);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.41e-01

CGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTG  >  Exported/189583‑189719
                                                                     |                                                                    
cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg                                                                     >  1:2828260/1‑70 (MQ=255)
cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg                                                                     >  1:2389013/1‑70 (MQ=255)
cGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATAGCAGGCTTCGCTGATCCACATTAAAGGCGAg                                                                     <  1:3524215/70‑1 (MQ=255)
 gAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTa                                                                   >  1:4136647/1‑71 (MQ=255)
   aaCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATg                                                                 <  1:3577523/71‑1 (MQ=255)
    aCGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGa                                                                >  1:4396090/1‑71 (MQ=255)
      gCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGAc                                                               >  1:1814148/1‑70 (MQ=255)
         aTCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg                                                              >  1:3528236/1‑68 (MQ=255)
            gACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAgg                                                        >  1:969445/1‑71 (MQ=255)
                 acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAg                                                                     <  1:4881224/53‑1 (MQ=255)
                 acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            >  1:4640438/1‑62 (MQ=255)
                 acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt                                                   <  1:5283223/71‑1 (MQ=255)
                 acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCttt                                                   <  1:1393737/71‑1 (MQ=255)
                 acaAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCtt                                                    >  1:165013/1‑70 (MQ=255)
                  caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg                                                              <  1:5583990/59‑1 (MQ=255)
                  caAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTa                                                  <  1:5347006/71‑1 (MQ=255)
                  caAAATGCTGGCGGCGGTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGAc                                                               <  1:3759399/58‑1 (MQ=255)
                    aactGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAAc                                                <  1:3267072/68‑1 (MQ=255)
                    aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACg                                                              <  1:4168114/57‑1 (MQ=255)
                    aaaTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            >  1:4537899/1‑59 (MQ=255)
                     actGCTGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAAc                                                >  1:2664385/3‑70 (MQ=255)
                      aTGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGa                                              <  1:1993556/71‑1 (MQ=255)
                          tGGCGGCGTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGcc                                          >  1:5052938/1‑71 (MQ=9)
                            gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCta                                        <  1:3187422/71‑2 (MQ=9)
                            gcggcgTTCTCTCAGGCTTCCATGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCta                                        <  1:1625746/71‑2 (MQ=9)
                             cggcgTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCttt                                       <  1:3467769/71‑1 (MQ=255)
                                 gTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatga                                   <  1:1687361/71‑1 (MQ=255)
                                    aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            >  1:374156/1‑43 (MQ=255)
                                    aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg                                 >  1:5344576/1‑70 (MQ=255)
                                    aTCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatgatg                                 >  1:200255/1‑70 (MQ=255)
                                     tCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            <  1:676933/42‑1 (MQ=255)
                                     tCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            <  1:2421135/42‑1 (MQ=255)
                                     tCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGaa                                                            <  1:4873787/42‑1 (MQ=255)
                                       gCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAaga                                                          >  1:952053/1‑42 (MQ=255)
                                       gCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAaga                                                          >  1:3115107/1‑42 (MQ=255)
                                       gCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTat                                     >  1:2311735/1‑63 (MQ=255)
                                         aGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAagag                                                         >  1:810037/1‑41 (MQ=255)
                                          ggCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACg                                               >  1:4697611/1‑50 (MQ=255)
                                          ggCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGa                                              >  1:3355102/1‑51 (MQ=255)
                                          ggCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATacca                          >  1:5404280/1‑71 (MQ=255)
                                             ttCGCTGATCCACATTAAAGGCGAGTATGACGAagag                                                         >  1:260674/1‑37 (MQ=255)
                                             ttCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATac                            >  1:1271987/1‑66 (MQ=255)
                                                gCTGATCCACAATAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccaccacc                     <  1:4619047/70‑1 (MQ=255)
                                                 cTGATCCACATTAAAGGCGATTATGACGAAGAGGCCTTTAACGAAGCCTTTatgat                                  <  1:224533/56‑1 (MQ=255)
                                                 atGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc                   <  1:4488805/70‑1 (MQ=9)
                                                 atGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc                   <  1:4482478/70‑1 (MQ=9)
                                                 atGATCCACGTTAAAGGTGACGTAAACGAAGAAACCTTTAACGAAGCCTACATGATGCACACCACCACTTc                   >  1:3772016/2‑71 (MQ=9)
                                                     tCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGccc               <  1:5266652/71‑1 (MQ=255)
                                                     tCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGccc               <  1:3725837/71‑1 (MQ=255)
                                                     tCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGcc                <  1:1177215/70‑1 (MQ=255)
                                                      ccACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTatga                                   >  1:4814425/1‑50 (MQ=255)
                                                           ttAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGc                                           <  1:5376660/37‑1 (MQ=255)
                                                             aaaGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATc        >  1:5594564/1‑70 (MQ=255)
                                                                 gCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAtt   >  1:3416724/1‑71 (MQ=255)
                                                                  cGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAttg  <  1:3962792/71‑1 (MQ=255)
                                                                  cGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCAtt   <  1:3617773/70‑1 (MQ=255)
                                                                   gAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATaccacca                       >  1:3986262/1‑49 (MQ=255)
                                                                     |                                                                    
CGAAACGCAATCGACCCACAAAATGCTGGCGGCGTTATCGCAGGCTTCGCTGATCCACATTAAAGGCGAGTATGACGAAGAGGCCTTTAACGAAGCCTTTATGATGCATACCACCACCTCGCCCAGTTATCCCATTG  >  Exported/189583‑189719

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: