| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 5,563 | G→A | 15.4% | intergenic (+33/+120) | yaaX → / ← yaaA | hypothetical protein/conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 5,563 | 0 | G | A | 15.4% | 89.2 / 8.2 | 39 | intergenic (+33/+120) | yaaX/yaaA | hypothetical protein/conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base G (20/13); new base A (4/2); total (24/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.61e-01 | |||||||||||
ACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCT > Exported/5496‑5632 | aCGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAgcgc > 1:4793265/1‑71 (MQ=255) aTGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGa > 1:168099/1‑71 (MQ=255) tGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGAt > 1:3303570/1‑66 (MQ=255) tGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGAc > 1:5011463/1‑71 (MQ=255) tCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGAt > 1:1736092/1‑63 (MQ=255) cAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTgg > 1:4560764/1‑71 (MQ=255) cAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTg < 1:4487782/70‑1 (MQ=255) cAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTg < 1:1934310/70‑1 (MQ=255) aCCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCa < 1:82975/71‑1 (MQ=255) cGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCt < 1:681862/44‑1 (MQ=255) aaaTGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTa > 1:4630571/1‑71 (MQ=255) gACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGt > 1:3674851/1‑70 (MQ=255) aCAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGtt < 1:3212405/70‑1 (MQ=255) aCAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTa < 1:3452523/71‑1 (MQ=255) cAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa > 1:4630314/1‑71 (MQ=255) cAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTaa < 1:4412898/71‑1 (MQ=255) gCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTg > 1:1866947/1‑71 (MQ=255) ggTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTg < 1:53032/67‑1 (MQ=255) ggTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTg < 1:910727/67‑1 (MQ=255) gTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGTCGCGTCTTAt > 1:2487018/1‑47 (MQ=255) gTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTAt > 1:3831613/1‑47 (MQ=255) tAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGt > 1:5568036/1‑41 (MQ=255) cgcatccGGTATGTAACGCCTGATGCGACGCTGACGCGTCTTATCTGGCCTACacgctgcgattttgtagg > 1:5540228‑M1/4‑53 (MQ=255) aCAATCAGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAatatat > 1:740861/1‑71 (MQ=255) cAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATAtt < 1:2741661/71‑1 (MQ=255) aTCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTg > 1:5468280/1‑70 (MQ=255) aTCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGa < 1:4792640/71‑1 (MQ=255) cGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAgg > 1:340860/1‑42 (MQ=255) cGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATc > 1:4198018/1‑71 (MQ=255) cGGCATTCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggat < 1:3323539‑M1/71‑25 (MQ=255) ggCATTCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACgcgctgcgattttgtaggccggat > 1:3154301‑M1/1‑46 (MQ=255) gCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTg > 1:1033395/1‑71 (MQ=255) cATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTg > 1:3429588/1‑70 (MQ=255) cATTCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggataag < 1:577759‑M1/71‑28 (MQ=255) ttCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCt < 1:4489936/67‑1 (MQ=255) ttCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccggataagc > 1:264260‑M1/1‑42 (MQ=255) ttCAACGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACacgctgcgattttgtaggccgg > 1:5316084‑M1/1‑42 (MQ=255) tCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCt < 1:5617861/39‑1 (MQ=255) tCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCAt > 1:1566045/1‑70 (MQ=255) aGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCt > 1:626255/1‑71 (MQ=255) | ACGGCGGTCATGGTCCAGGCAAACATCACCGCTAAATGACAAATGCCGGGTAACAATCCGGCATTCAGCGCCTGATGCGACGCTGGCGCGTCTTATCAGGCCTACGTTAATTCTGCAATATATTGAATCTGCATGCT > Exported/5496‑5632 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |