Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 834,956 C→A 19.9% V1138V (GTC→GTA narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported834,9560CA19.9% 237.8 / 33.6 95V1138V (GTC→GTAnarGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (37/39);  new base A (14/5);  total (51/44)
Fisher's exact test for biased strand distribution p-value = 7.15e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.14e-01

GTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCAT  >  Exported/834891‑835024
                                                                 |                                                                    
attgtcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCaa                                                                 >  1:1820043/4‑71 (MQ=255)
  tgtcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcg                                                               >  1:2343164/2‑70 (MQ=255)
   gtcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAg                                                              >  1:1649641/1‑71 (MQ=255)
   gtcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgc                                                              >  1:899990/1‑69 (MQ=255)
    tcTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGc                                                             <  1:4850177/71‑1 (MQ=255)
    tcTGGATCAGCGAAACAGATGCCCGAGAACTGACCATTGTCGATAACGACTGGGTGGAAGTATTCAAcgc                                                              >  1:3427809/1‑68 (MQ=255)
      tGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCaa                                                           >  1:2835637/1‑71 (MQ=255)
        gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCaa                                                           <  1:948975/69‑1 (MQ=255)
        gTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCaa                                                           <  1:3389306/69‑1 (MQ=255)
         ttGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCaa                                                           <  1:3434384/68‑1 (MQ=255)
           gAGTGAAGCCGATGCCAAAGATCTTGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTg                                                      >  1:4234490/1‑71 (MQ=255)
           gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTg                                                      >  1:4294503/1‑71 (MQ=255)
           gAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTg                                                      >  1:2770190/1‑71 (MQ=255)
             gTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGct                                                    <  1:3521631/71‑1 (MQ=255)
              tGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGct                                                    <  1:1526458/70‑1 (MQ=255)
                aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                  >  1:4765087/1‑70 (MQ=255)
                aaGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                  >  1:4154054/1‑70 (MQ=255)
                       tGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTc                                                                   <  1:207929/46‑1 (MQ=255)
                       tGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTc                                                                   <  1:2445789/46‑1 (MQ=255)
                       tGCCAAAGATCTAGGTATCGCCGATAACGACTGGATTGAAGTCTTc                                                                   <  1:5360145/46‑1 (MQ=255)
                         ccAAAGATCTGGGTATCGCCGTTAACGACTGGATTGAAGTCTTCAACAGCAACGGTTCTCTGACTGCCCGt                                        <  1:618572/71‑1 (MQ=255)
                         ccAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGt                                        >  1:2396364/1‑71 (MQ=255)
                          cAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCa                                                            <  1:4976647/50‑1 (MQ=255)
                          cAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGt                                        >  1:158524/1‑70 (MQ=255)
                           aaaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTc                                                                   <  1:386399/42‑1 (MQ=255)
                           aaaGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGc                                      >  1:1479942/1‑71 (MQ=255)
                             aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCaa                                                                 >  1:875753/1‑42 (MQ=255)
                             aGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCgg                                    <  1:3748388/71‑1 (MQ=255)
                             aGATCTGGGTATCGCCGAAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGt                                   >  1:133434/1‑71 (MQ=255)
                              gATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTc                                                                   >  1:1729848/1‑39 (MQ=255)
                              gATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGt                                   <  1:3713258/71‑1 (MQ=255)
                               aTCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCaa                                                           <  1:815557/46‑1 (MQ=255)
                                 cTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTg                                 >  1:1525425/1‑70 (MQ=255)
                                 cTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTg                                 >  1:4659357/1‑70 (MQ=255)
                                 cTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTg                                 >  1:1986201/1‑70 (MQ=255)
                                   gggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCaa                                                           <  1:2345155/42‑1 (MQ=255)
                                   gggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGt                                >  1:3106948/1‑69 (MQ=255)
                                    ggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACgg                                                        >  1:64356/1‑44 (MQ=255)
                                    ggTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACgg                                                        >  1:834707/1‑44 (MQ=255)
                                     gTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc                            <  1:4109995/71‑1 (MQ=255)
                                     gTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagc                            <  1:2836867/71‑1 (MQ=255)
                                      tATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGtt                                  <  1:5235198/64‑1 (MQ=255)
                                        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCa                                                            <  1:1963822/36‑1 (MQ=255)
                                        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCa                                                            <  1:5236720/36‑1 (MQ=255)
                                        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCa                                                            <  1:3806446/36‑1 (MQ=255)
                                        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                  <  1:5433227/46‑1 (MQ=255)
                                        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                  <  1:2095486/46‑1 (MQ=255)
                                        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                  <  1:5548519/46‑1 (MQ=255)
                                        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                  <  1:4329922/46‑1 (MQ=255)
                                        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGctct                                                  <  1:946040/46‑1 (MQ=255)
                                        tCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTcagccag                         >  1:3045687/1‑71 (MQ=255)
                                          gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACt                                              >  1:433713/3‑48 (MQ=12)
                                          gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACt                                              >  1:3904438/3‑48 (MQ=12)
                                          gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTca                              >  1:2057095/3‑64 (MQ=255)
                                          gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACg                       >  1:1991539/3‑71 (MQ=255)
                                          gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACg                       >  1:5386486/3‑71 (MQ=255)
                                          gtcGATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACg                       >  1:3982122/3‑71 (MQ=255)
                                          gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGGCAGCg                       >  1:1210870/1‑71 (MQ=255)
                                          gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCg                       >  1:1421242/1‑71 (MQ=255)
                                          gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCg                       >  1:4128563/1‑71 (MQ=255)
                                          gCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCg                       >  1:5591984/1‑71 (MQ=255)
                                             gATAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtg                     <  1:3609679/70‑1 (MQ=255)
                                              aTAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtgta                   <  1:1640830/71‑2 (MQ=255)
                                              aTAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGtt                   >  1:1871631/1‑71 (MQ=255)
                                               tAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtgtac                  >  1:1578511/1‑69 (MQ=255)
                                               tAACGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtgtac                  >  1:25384/1‑69 (MQ=255)
                                               tAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTc                  <  1:4348712/71‑1 (MQ=255)
                                                aaCGACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTAcc                 >  1:5064802/1‑71 (MQ=255)
                                                aaCGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTcc                 >  1:4437493/1‑71 (MQ=255)
                                                aaCGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTcc                 >  1:4115595/1‑71 (MQ=255)
                                                   gACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtg                     <  1:4463087/64‑1 (MQ=255)
                                                   gACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtg                     <  1:2911574/64‑1 (MQ=255)
                                                   gACTGGGTGGAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACgtg                     <  1:4456176/64‑1 (MQ=255)
                                                   gACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc              <  1:3507533/71‑1 (MQ=255)
                                                    aCTGGATTGAAGTCTTCAACAGAAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCa             <  1:4126060/71‑1 (MQ=255)
                                                     cTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAg            >  1:1206532/1‑71 (MQ=255)
                                                        gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGt                                >  1:5522397/1‑48 (MQ=255)
                                                        gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg           <  1:1166388/69‑1 (MQ=255)
                                                        gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg           <  1:234117/69‑1 (MQ=255)
                                                        gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg           <  1:4852113/69‑1 (MQ=255)
                                                        gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg           <  1:4414455/69‑1 (MQ=255)
                                                        gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg           <  1:3770936/69‑1 (MQ=255)
                                                        gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg           <  1:3777645/69‑1 (MQ=255)
                                                        gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGa         >  1:5364934/1‑71 (MQ=255)
                                                        gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGa         >  1:3396998/1‑71 (MQ=255)
                                                         aTTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGt                                   >  1:2849532/1‑44 (MQ=255)
                                                            gAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGAc     >  1:2012149/1‑71 (MQ=255)
                                                             aaGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGAc     <  1:388643/70‑1 (MQ=255)
                                                              aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGa      >  1:1540180/1‑68 (MQ=255)
                                                              aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa   <  1:5112215/71‑1 (MQ=255)
                                                              aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa   <  1:114199/71‑1 (MQ=255)
                                                               gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat  >  1:4671006/1‑71 (MQ=255)
                                                               gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat  >  1:1337076/1‑71 (MQ=255)
                                                               gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat  >  1:277424/1‑71 (MQ=255)
                                                               gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat  >  1:4921961/1‑71 (MQ=255)
                                                                 |                                                                    
GTGGTCTGGTTGAGTGAAGCCGATGCCAAAGATCTGGGTATCGCCGATAACGACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCAT  >  Exported/834891‑835024

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: