Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 835,011 G→C 21.6% A1157P (GCA→CCA) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported835,0110GC21.6% 256.2 / 65.0 125A1157P (GCA→CCA) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (44/54);  new base C (16/11);  total (60/65)
Fisher's exact test for biased strand distribution p-value = 2.00e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.61e-01

Only 100 of 125 total aligned reads displayed.

GACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAA  >  Exported/834942‑835079
                                                                     |                                                                    
gACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc                                                                     <  1:3507533/71‑1 (MQ=255)
     gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                                  <  1:234117/69‑1 (MQ=255)
     gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                                  <  1:4852113/69‑1 (MQ=255)
     gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                                  <  1:4414455/69‑1 (MQ=255)
     gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                                  <  1:1166388/69‑1 (MQ=255)
     gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                                  <  1:3777645/69‑1 (MQ=255)
     gATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGa                                                                >  1:3396998/1‑71 (MQ=255)
         gAAGTATTCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGAc                                                            >  1:2012149/1‑71 (MQ=255)
          aaGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGAc                                                            <  1:388643/70‑1 (MQ=255)
           aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGa                                                             >  1:1540180/1‑68 (MQ=255)
           aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                                          <  1:114199/71‑1 (MQ=255)
           aGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                                          <  1:5112215/71‑1 (MQ=255)
            gtattCAACGCCAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCa                                                          >  1:4559720/4‑70 (MQ=255)
            gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                                         >  1:277424/1‑71 (MQ=255)
            gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                                         >  1:1337076/1‑71 (MQ=255)
            gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                                         >  1:4671006/1‑71 (MQ=255)
            gTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCat                                                         >  1:4921961/1‑71 (MQ=255)
               ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                                  <  1:3374290/59‑1 (MQ=255)
               ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAgg                                                                  <  1:828517/59‑1 (MQ=255)
               ttCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgat                                                      >  1:1140239/1‑71 (MQ=255)
                 cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGAt                                                               >  1:783057/1‑60 (MQ=255)
                 cAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGAt                                                               >  1:1074713/1‑60 (MQ=255)
                    cAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAcc                                                 <  1:3832943/71‑1 (MQ=255)
                     gccAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCa                                                >  1:1251324/3‑71 (MQ=255)
                      ccAACGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAc                                               >  1:4609626/2‑71 (MQ=255)
                        aaCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgc                                             >  1:906405/1‑71 (MQ=255)
                        aaCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgc                                             >  1:828027/1‑71 (MQ=255)
                         aCGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAg                                                                   <  1:3711934/48‑1 (MQ=255)
                         aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTat                                                  >  1:1890195/1‑64 (MQ=255)
                         aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTat                                                  >  1:2962100/1‑64 (MQ=255)
                         aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTat                                                  >  1:2708417/1‑64 (MQ=255)
                         aCGGCGCGCTGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCAcgcc                                            >  1:3788152/1‑70 (MQ=255)
                           ggTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCAcgcg                                            >  1:4350576/1‑69 (MQ=255)
                            gTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAg                                         <  1:1291937/71‑1 (MQ=255)
                             tGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAgg                                        <  1:859508/71‑1 (MQ=255)
                               cgctGACTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa                                      <  1:412202/69‑1 (MQ=255)
                                tctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGc                                                                     <  1:5557475/39‑1 (MQ=255)
                                 ctGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg                                    >  1:447976/1‑71 (MQ=255)
                                  tGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTTCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg                                    >  1:4915988/1‑70 (MQ=255)
                                   gACTGCCCGTGCGGTTGTCAGGCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa                                  >  1:4388736/1‑71 (MQ=255)
                                   gACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTa                                  >  1:2540680/1‑71 (MQ=255)
                                    aCTGCCCGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAt                                 >  1:91440/1‑71 (MQ=255)
                                      tGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                                     <  1:1829981/49‑1 (MQ=255)
                                        cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCg                               <  1:661469/69‑1 (MQ=255)
                                        cccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt                             <  1:4116101/71‑1 (MQ=255)
                                        cccGCGCGGTGGTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAttat                              <  1:3019946/70‑4 (MQ=255)
                                        cccGCGCGGTGGTCAACCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCAttat                              >  1:45183/1‑67 (MQ=255)
                                         ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt                                   <  1:1621651/64‑1 (MQ=255)
                                         ccGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGt                                   <  1:3707454/64‑1 (MQ=255)
                                            tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                                          <  1:2461041/38‑1 (MQ=255)
                                            tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCa                                                          <  1:2566865/38‑1 (MQ=255)
                                            tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa                                                   >  1:641543/1‑45 (MQ=255)
                                            tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTa                                                   >  1:293482/1‑45 (MQ=255)
                                            tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt                             <  1:2860641/67‑1 (MQ=255)
                                            tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGtt                             <  1:679706/67‑1 (MQ=255)
                                            tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc                         >  1:2168996/1‑71 (MQ=255)
                                             gCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcct                        >  1:1216563/1‑71 (MQ=255)
                                              cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg                       >  1:3045363/1‑71 (MQ=255)
                                              cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg                       >  1:4223642/1‑71 (MQ=255)
                                                gTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTAc                                                  <  1:4478773/42‑1 (MQ=255)
                                                 ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct                    <  1:2602551/71‑1 (MQ=255)
                                                 ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct                    <  1:1044911/71‑1 (MQ=255)
                                                 ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct                    <  1:3365965/71‑1 (MQ=255)
                                                   gTCAGCCAGCGTGTTCCGGCAGGGATGACCatgatg                                                     >  1:1405320/1‑36 (MQ=255)
                                                   gTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACg                                    >  1:1033878/1‑53 (MQ=255)
                                                   gTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTgg                  >  1:4585703/1‑71 (MQ=255)
                                                   gTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCa                                          <  1:2518405/47‑1 (MQ=16)
                                                   gTCAGCCAACGTGTACCGCCGGGCATGACCATGATGTATCACGCCCa                                          <  1:3277177/47‑1 (MQ=16)
                                                     cagccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGtt                >  1:4710332/1‑71 (MQ=255)
                                                     cagccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAgg                                        >  1:2456129/1‑47 (MQ=16)
                                                     cagccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACgc                                   >  1:5210683/1‑51 (MQ=16)
                                                      agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc               >  1:832669/1‑71 (MQ=255)
                                                      agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc               >  1:839341/1‑71 (MQ=255)
                                                      agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc               >  1:2082186/1‑71 (MQ=255)
                                                      agccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTc               >  1:3183756/1‑71 (MQ=255)
                                                       gccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa                            <  1:2997136/57‑1 (MQ=255)
                                                       gccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa                            <  1:4065311/57‑1 (MQ=255)
                                                       gccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTa                            <  1:5384197/57‑1 (MQ=255)
                                                        ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg             <  1:4492449/71‑1 (MQ=255)
                                                        ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg             <  1:641250/71‑1 (MQ=255)
                                                        ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGCTTCgg             <  1:2188890/71‑1 (MQ=255)
                                                          agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa            >  1:1111952/1‑70 (MQ=255)
                                                          agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa            <  1:5320507/70‑1 (MQ=255)
                                                          aacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCg              <  1:1771948/66‑1 (MQ=255)
                                                           acGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGaa                                      >  1:4853511/2‑43 (MQ=12)
                                                            cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg             <  1:5510869/67‑1 (MQ=255)
                                                            cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg             <  1:3043868/67‑1 (MQ=255)
                                                            cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg             <  1:2805172/67‑1 (MQ=255)
                                                             gtgtACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagta        <  1:4552860/71‑4 (MQ=255)
                                                             gtgtACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagt         >  1:540723/1‑68 (MQ=255)
                                                              tgtTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGt                              <  1:5269924/48‑1 (MQ=255)
                                                              tgtTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGt                              <  1:4029514/48‑1 (MQ=255)
                                                                ttCCGGCAGGGATGACCATGATGTACCACGCGCAg                                         >  1:5210523/1‑35 (MQ=255)
                                                                ttCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa            <  1:3109862/64‑1 (MQ=255)
                                                                ttCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAcc     >  1:3714975/1‑71 (MQ=255)
                                                                  ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa          >  1:5110952/1‑64 (MQ=255)
                                                                  ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc    >  1:1956105/1‑70 (MQ=255)
                                                                  ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc    >  1:1409111/1‑70 (MQ=255)
                                                                   cGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCaa  <  1:100192/71‑1 (MQ=255)
                                                                   cGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCa   <  1:2256105/70‑1 (MQ=255)
                                                                     |                                                                    
GACTGGATTGAAGTCTTCAACAGCAACGGTGCTCTGACTGCCCGTGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAA  >  Exported/834942‑835079

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: