Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 835,056 C→A 10.5% L1172M (CTG→ATG) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported835,0560CA10.5% 298.3 / 13.4 95L1172M (CTG→ATG) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (39/46);  new base A (2/8);  total (41/54)
Fisher's exact test for biased strand distribution p-value = 1.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.40e-01

Only 100 of 110 total aligned reads displayed.

TGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAAC  >  Exported/834986‑835125
                                                                      |                                                                     
tGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc                                                                       >  1:2168996/1‑71 (MQ=255)
 gCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcct                                                                      >  1:1216563/1‑71 (MQ=255)
  cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg                                                                     >  1:4223642/1‑71 (MQ=255)
  cGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctg                                                                     >  1:3045363/1‑71 (MQ=255)
     ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct                                                                  <  1:1044911/71‑1 (MQ=255)
     ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct                                                                  <  1:3365965/71‑1 (MQ=255)
     ttGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAAcctgcct                                                                  <  1:2602551/71‑1 (MQ=255)
       gTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTgg                                                                >  1:4585703/1‑71 (MQ=255)
         cagccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGtt                                                              >  1:4710332/1‑71 (MQ=255)
          agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc                                                             >  1:832669/1‑71 (MQ=255)
          agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc                                                             >  1:2082186/1‑71 (MQ=255)
          agccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTc                                                             >  1:168575/1‑71 (MQ=255)
          agccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTc                                                             >  1:3183756/1‑71 (MQ=255)
            ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg                                                           <  1:4492449/71‑1 (MQ=255)
            ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg                                                           <  1:641250/71‑1 (MQ=255)
            ccagcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGCTTCgg                                                           <  1:2188890/71‑1 (MQ=255)
            ccaacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCgg                                                           <  1:3500068/71‑1 (MQ=255)
              agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa                                                          >  1:1111952/1‑70 (MQ=255)
              agcGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa                                                          <  1:5320507/70‑1 (MQ=255)
              aacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCg                                                            <  1:1771948/66‑1 (MQ=255)
              aacGTGTACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaa                                                         <  1:4544682/69‑1 (MQ=255)
                cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg                                                           <  1:3043868/67‑1 (MQ=255)
                cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg                                                           <  1:2805172/67‑1 (MQ=255)
                cGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCgg                                                           <  1:5510869/67‑1 (MQ=255)
                 gtgtACCGCCGGGCATGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagt                                                       >  1:540723/1‑68 (MQ=255)
                    ttCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa                                                          <  1:3109862/64‑1 (MQ=255)
                    ttCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAcc                                                   >  1:3714975/1‑71 (MQ=255)
                      ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa                                                        >  1:630580/1‑64 (MQ=255)
                      ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa                                                        >  1:5110952/1‑64 (MQ=255)
                      ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc                                                  >  1:5149989/1‑70 (MQ=255)
                      ccGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAccc                                                  <  1:266157/70‑1 (MQ=255)
                       cGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCaa                                                <  1:100192/71‑1 (MQ=255)
                       cGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCa                                                 <  1:2256105/70‑1 (MQ=255)
                          cAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAg                                             <  1:5439251/71‑1 (MQ=255)
                           aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCaa                                                >  1:3655850/1‑67 (MQ=255)
                           aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc                                            <  1:711814/71‑1 (MQ=255)
                           aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc                                            <  1:2919289/71‑1 (MQ=255)
                           aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc                                            <  1:4290125/71‑1 (MQ=255)
                           aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc                                            >  1:5220430/1‑71 (MQ=255)
                           aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc                                            >  1:2105595/1‑71 (MQ=255)
                           aGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGc                                            <  1:3324322/71‑1 (MQ=255)
                            gggATGACCATGATGTACCACGCGCAGTAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCCACAGCg                                           <  1:740230/71‑1 (MQ=255)
                            gggATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAGATTACCCAACAGCg                                           <  1:1989126/71‑1 (MQ=255)
                            gggATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg                                           <  1:1873344/71‑1 (MQ=255)
                            gggATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg                                           >  1:32498/1‑71 (MQ=255)
                               aTGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCgtgg                                        <  1:4157222/71‑1 (MQ=255)
                               aTGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCgtgg                                        <  1:1870127/71‑1 (MQ=255)
                                tGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagt                                                       <  1:5571842/55‑3 (MQ=255)
                                tGACCATGATGTATCACGCCCAGGAACGCATTATGAATATTCCTGGTTCGGaagt                                                       <  1:2520382/55‑3 (MQ=255)
                                 gACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc                                                                       <  1:1715527/38‑1 (MQ=255)
                                 gACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc                                                                       <  1:4424211/38‑1 (MQ=255)
                                 gACCATGATGTACCACGCGCAGGAACGTATCGTTAAcc                                                                       <  1:4516214/38‑1 (MQ=255)
                                  aCCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTAc                                                    >  1:3980459/1‑56 (MQ=255)
                                   ccATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTggtggt                                    <  1:3594538/71‑1 (MQ=255)
                                      tgatgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa                                                          <  1:5377771/46‑1 (MQ=255)
                                      tgatgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGtggtgg                                     >  1:3078849/1‑67 (MQ=255)
                                      tgatgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATc                                 >  1:4629983/1‑71 (MQ=255)
                                        atgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATcc                                <  1:4719251/70‑1 (MQ=255)
                                        atgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATcc                                <  1:5501726/70‑1 (MQ=255)
                                         tgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGa                                                          <  1:1175358/43‑1 (MQ=255)
                                         tgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCAt                              <  1:2338144/71‑1 (MQ=255)
                                         tgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCAt                              <  1:1066530/71‑1 (MQ=255)
                                         tgTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCAt                              <  1:755176/71‑1 (MQ=255)
                                          gTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCAt                              >  1:1364143/1‑70 (MQ=255)
                                           tACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATaa                            >  1:4669266/1‑71 (MQ=255)
                                             ccACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACt                          <  1:1293248/71‑1 (MQ=255)
                                              cACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGaaa                                                        >  1:2583663/1‑40 (MQ=255)
                                              cACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCa                               <  1:4578529/65‑1 (MQ=255)
                                               aCGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCg                        >  1:316830/1‑71 (MQ=255)
                                                cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCgt                                          >  1:656371/1‑52 (MQ=255)
                                                cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg                                           <  1:1304411/51‑1 (MQ=255)
                                                cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg                                           <  1:3891796/51‑1 (MQ=255)
                                                cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCg                                           <  1:347520/51‑1 (MQ=255)
                                                cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCgg                       >  1:4904930/1‑71 (MQ=255)
                                                cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCgg                       >  1:3692524/1‑71 (MQ=255)
                                                cgcgCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCgg                       >  1:3817086/1‑71 (MQ=255)
                                                   gcAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCa                    >  1:179856/1‑71 (MQ=255)
                                                      ggAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCAccc                 >  1:1694899/1‑71 (MQ=255)
                                                       gAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTAt                                  <  1:793558/53‑1 (MQ=255)
                                                       gAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCg                >  1:2103356/1‑71 (MQ=255)
                                                       gAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCg                >  1:4177929/1‑71 (MQ=255)
                                                           gTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTAt             >  1:3750120/1‑70 (MQ=255)
                                                            tATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCgtgg                                        <  1:3339139/42‑1 (MQ=255)
                                                            tATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCgtgg                                        <  1:2803009/42‑1 (MQ=255)
                                                              tCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACg         <  1:1602778/71‑1 (MQ=255)
                                                              tCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACg         <  1:2958272/71‑1 (MQ=255)
                                                               cGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACg         <  1:3288410/70‑1 (MQ=255)
                                                                 ttAACCTGCCTGGTTCGGAAATTATCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTAt             <  1:5608505/64‑1 (MQ=255)
                                                                 ttAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCg      <  1:4973534/71‑1 (MQ=255)
                                                                  tAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTa                                   >  1:4839872/1‑41 (MQ=255)
                                                                  tAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCg      >  1:2134356/1‑70 (MQ=255)
                                                                  tAACCTGCCTGGTTCAGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCg      >  1:3192553/1‑70 (MQ=255)
                                                                   aaCCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCa           >  1:4818451/1‑64 (MQ=255)
                                                                    aCCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGaaa   <  1:1100659/71‑1 (MQ=255)
                                                                    aCCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGaa    <  1:5181820/70‑1 (MQ=255)
                                                                     cctgcctgGTTCGGAAATTACCCAACAGCGTGGTGGTATcc                                <  1:1934334/41‑1 (MQ=255)
                                                                     cctgcctgGTTCGGAAATTACCCAACAGCGTGGTGGTATcc                                <  1:4446867/41‑1 (MQ=255)
                                                                     cctgcctgGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGt               <  1:339870/58‑1 (MQ=255)
                                                                     cctgcctgGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGt               <  1:1697371/58‑1 (MQ=255)
                                                                      ctgcctgGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAAc  >  1:2746073/1‑70 (MQ=255)
                                                                      |                                                                     
TGCGGTTGTCAGCCAGCGTGTTCCGGCAGGGATGACCATGATGTACCACGCGCAGGAACGTATCGTTAACCTGCCTGGTTCGGAAATTACCCAACAGCGTGGTGGTATCCATAACTCGGTCACCCGTATCACGCCGAAAC  >  Exported/834986‑835125

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: