| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 190,111 | C→G | 44.5% | L536L (CTC→CTG) | ldcC → | lysine decarboxylase 2, constitutive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 190,111 | 0 | C | G | 44.5% | 29.9 / 89.9 | 72 | L536L (CTC→CTG) | ldcC | lysine decarboxylase 2, constitutive |
| Reads supporting (aligned to +/- strand): ref base C (23/17); new base G (8/24); total (31/41) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.70e-01 | |||||||||||
TGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGC > Exported/190041‑190177 | tGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTtctc > 1:5501753/1‑71 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTa < 1:4080404/69‑1 (MQ=255) cAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAg > 1:3135692/1‑70 (MQ=255) gaaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:2563564/70‑3 (MQ=255) gaaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAgca < 1:1921034/70‑3 (MQ=255) aaaaTTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTa > 1:5446262/1‑71 (MQ=255) aaaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGttc < 1:212265/66‑2 (MQ=255) aaTACCTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc > 1:3633037/1‑70 (MQ=255) ttCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTgg > 1:705990/1‑71 (MQ=255) taccTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCgg < 1:164118/69‑1 (MQ=255) cTCGACGAACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTAt > 1:880512/1‑71 (MQ=255) cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTAt < 1:973463/63‑1 (MQ=255) cgacgaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATc > 1:682283/1‑70 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATc < 1:288101/69‑1 (MQ=255) gacgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATc < 1:4136023/69‑1 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGa > 1:3479110/1‑70 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:3428638/71‑1 (MQ=255) acgaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:2344690/71‑1 (MQ=255) gaACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATa > 1:4932308/1‑70 (MQ=255) gaACATGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc > 1:1705181/1‑59 (MQ=12) aCGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGc > 1:4708487/1‑61 (MQ=255) atGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGAcc < 1:4827765/70‑1 (MQ=255) atGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGAcc < 1:719281/70‑1 (MQ=255) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:1265055/62‑1 (MQ=12) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:4706954/62‑1 (MQ=12) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCg < 1:1373705/62‑1 (MQ=12) tGGCATCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCa < 1:1566860/71‑1 (MQ=255) ggATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa > 1:2260222/1‑69 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCAtc < 1:608460/51‑2 (MQ=12) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:5253021/70‑1 (MQ=255) caTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:2596964/70‑1 (MQ=255) aTCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGc < 1:2341838/71‑1 (MQ=255) tCGTTGTTGAGAAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAg < 1:5287639/46‑1 (MQ=12) tCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCa < 1:1695552/71‑1 (MQ=255) ttgagaAAACCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt < 1:4744508/51‑1 (MQ=255) agagAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGat > 1:2420110/1‑71 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaaa > 1:4133317/1‑54 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCaaa > 1:644475/1‑60 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCaaa > 1:2881683/1‑60 (MQ=255) gagaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGatt < 1:2332978/71‑1 (MQ=255) agaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTa < 1:4684909/39‑1 (MQ=255) agaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTa < 1:4322470/39‑1 (MQ=255) gaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTa > 1:4259462/1‑38 (MQ=255) gaAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCaa < 1:314931/62‑1 (MQ=255) aaaaCCGGGCCTTATAACCTGCTGTTTCTCTTTAGTa < 1:5341206/37‑1 (MQ=255) aaaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTAt > 1:5043411/1‑38 (MQ=255) aaaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGAc < 1:299019/52‑1 (MQ=255) aaaCCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTg < 1:1636107/71‑1 (MQ=255) aaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGa > 1:3036080/1‑45 (MQ=255) aaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGAt > 1:2727915/1‑46 (MQ=255) aaCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCa > 1:3654314/1‑58 (MQ=255) aCCGGTCCGTATAACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGCac > 1:4835337/1‑57 (MQ=255) gCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGatt < 1:4273272/61‑1 (MQ=255) ccTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGtt < 1:4242036/71‑1 (MQ=255) cTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATaa < 1:3085292/40‑1 (MQ=255) ttATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGt < 1:20500/64‑1 (MQ=255) gtataACCTGCTTTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGc < 1:2075650/47‑1 (MQ=255) gtataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAGc < 1:3049035/47‑1 (MQ=255) tataACCTGCTGTTTCTCTTTAGTATTGGCATCGAt > 1:4151991/1‑36 (MQ=255) tataACCTGCTGTTTCTCTTTAGTATTGGCATCGAt > 1:2871087/1‑36 (MQ=255) tataACCTGCTGTTTCTCTTTAGTATTGGCATCGAt > 1:5527699/1‑36 (MQ=255) tataACCTGCTGTTCCTGTTCAGCATCGGTATCGATAAGACCAAAg < 1:1458781/46‑1 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa > 1:3481190/1‑42 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCa > 1:2140347/1‑42 (MQ=255) ataACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:3610225/1‑71 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg < 1:5092900/62‑1 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTg < 1:1997495/62‑1 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:3220666/1‑70 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:2942458/1‑70 (MQ=255) taACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACg > 1:2552889/1‑70 (MQ=255) aCCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGaa < 1:3546979/71‑1 (MQ=255) ctgTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCa < 1:5396298/70‑1 (MQ=255) ttCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGc > 1:981195/1‑71 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCaa > 1:3939650/1‑35 (MQ=255) tctcTTTAGTATTGGCATCGATAAAACCAAAGCaa > 1:372864/1‑35 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCg > 1:322455/1‑47 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCg > 1:1817460/1‑47 (MQ=255) ctctTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCg > 1:1604306/1‑47 (MQ=255) | TGGTAGCAAAATTCCTCGACGAACGTGGGATCGTAGTAGAGAAAACCGGCCCTTATAACCTGCTGTTTCTCTTTAGTATTGGCATCGATAAAACCAAAGCAATGGGATTATTGCGTGGGTTGACGGAATTCAAACGC > Exported/190041‑190177 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |