Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 835,215 G→A 23.2% V1225M (GTG→ATG) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported835,2150GA23.2% 214.6 / 58.5 116V1225M (GTG→ATG) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (40/49);  new base A (16/11);  total (56/60)
Fisher's exact test for biased strand distribution p-value = 2.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 120 total aligned reads displayed.

TATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAA  >  Exported/835146‑835283
                                                                     |                                                                    
tATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGt                                                                     >  1:235901/1‑71 (MQ=255)
    cccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt                                                                 <  1:140015/71‑1 (MQ=255)
     ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg                                                                      >  1:4510044/1‑65 (MQ=255)
     ccATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAg                                                                      >  1:4361515/1‑65 (MQ=255)
             cATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaag                                                        <  1:3865259/71‑1 (MQ=255)
                ggggCTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAc                                                     >  1:4667421/3‑71 (MQ=255)
                aCGGCTATAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaagaa                                                      >  1:730620/1‑70 (MQ=255)
                 cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                                    <  1:5282743/71‑1 (MQ=255)
                 cGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                                    >  1:5614683/1‑71 (MQ=255)
                   gCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                                  <  1:2893467/71‑1 (MQ=255)
                    cTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                                  >  1:3862488/1‑70 (MQ=255)
                    cTTTAACTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGtta                                                 <  1:3280931/71‑2 (MQ=255)
                        aaCTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACt                                              <  1:3100500/70‑1 (MQ=255)
                        aaCTACTACGGGACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATgaagaa                                                      >  1:5171552/1‑62 (MQ=255)
                        aaCTACTACGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATgaagaa                                                      >  1:3456343/1‑62 (MQ=255)
                         actactATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAt                                                   >  1:3442705/1‑64 (MQ=255)
                          ctactaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTgg                                            <  1:4001945/70‑1 (MQ=255)
                          ctactaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt                                           <  1:2812972/71‑1 (MQ=255)
                          ctactaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt                                           <  1:105446/71‑1 (MQ=255)
                          ctactaCGGCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTggc                                           <  1:3275169/71‑2 (MQ=255)
                             ctaTGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGt                                           >  1:4961987/1‑68 (MQ=255)
                               acggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt                                      >  1:256060/3‑71 (MQ=255)
                               acggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAACAACGTTAACTGGCTGGAt                                      >  1:359637/3‑71 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa                                                         >  1:1035745/1‑51 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATgaa                                                         >  1:3205365/1‑51 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                                    <  1:5095584/56‑1 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa                                       <  1:1103606/69‑1 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa                                       <  1:5171278/69‑1 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg                                     >  1:5242741/1‑71 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg                                     >  1:1580240/1‑71 (MQ=255)
                                tGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATg                                     >  1:1344664/1‑71 (MQ=255)
                                cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAAcg                                                    >  1:1312757/2‑55 (MQ=255)
                                cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGttaa                                                >  1:2452135/2‑58 (MQ=255)
                                cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt                                      <  1:3390824/69‑1 (MQ=255)
                                cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt                                      <  1:1590219/69‑1 (MQ=255)
                                cggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt                                      <  1:4956914/69‑1 (MQ=255)
                                cggCACCGTCGGATCGAACCGCGATGAGCTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGAt                                      >  1:283218/2‑70 (MQ=255)
                                 ggCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATga                                    <  1:5684417/71‑2 (MQ=255)
                                  gCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATgat                                   >  1:3678124/1‑69 (MQ=255)
                                  gCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATgat                                   >  1:2619988/1‑69 (MQ=255)
                                  gCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATgat                                   >  1:227615/1‑69 (MQ=255)
                                  gCACCGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATga                                    >  1:5190126/1‑69 (MQ=255)
                                  gCACCGTCGGATCGAACCGCGATGACTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATgat                                   <  1:733046/71‑3 (MQ=255)
                                  gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGAc                                               >  1:3683516/1‑59 (MQ=255)
                                  gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATgg                                    <  1:5495953/70‑1 (MQ=255)
                                  gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATgg                                    <  1:5209487/70‑1 (MQ=255)
                                   cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGc                                                                   <  1:1586383/38‑1 (MQ=255)
                                   cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGTAGAACATTGACTGGTTAGATGGCg                                  <  1:2297451/71‑1 (MQ=255)
                                   cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAg                                        >  1:5296291/1‑65 (MQ=255)
                                   cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAg                                        >  1:1898385/1‑65 (MQ=255)
                                   cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                  <  1:2292372/71‑1 (MQ=255)
                                    aCCGTAGGTTGTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                  <  1:4327716/70‑1 (MQ=255)
                                     ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                  >  1:2935844/1‑69 (MQ=255)
                                      cGTCGGATCGAACCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTgg                                            <  1:3136321/58‑1 (MQ=255)
                                      cGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGt                                                                 <  1:2258041/37‑1 (MQ=255)
                                       gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGa                                                             >  1:1300459/1‑40 (MQ=255)
                                       gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGGAGAACATTGACTGGTTa                                         >  1:2039359/1‑60 (MQ=255)
                                       gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                         >  1:1116169/1‑60 (MQ=255)
                                       gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAgg                              >  1:932341/1‑71 (MQ=255)
                                        tAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACa                                                    >  1:3197425/1‑48 (MQ=255)
                                           gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACAtt                                                  <  1:30916/47‑1 (MQ=255)
                                           gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                         <  1:5485123/56‑1 (MQ=255)
                                           gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                         <  1:1946457/56‑1 (MQ=255)
                                           gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                          <  1:959522/71‑1 (MQ=255)
                                           gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                          <  1:4239680/71‑1 (MQ=255)
                                            ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                                 <  1:3286952/63‑1 (MQ=255)
                                            ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                                 <  1:1123275/63‑1 (MQ=255)
                                             tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa                                       >  1:4934101/1‑56 (MQ=255)
                                             tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa                                       >  1:4039788/1‑56 (MQ=255)
                                             tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGc                                   <  1:4522556/60‑1 (MQ=255)
                                             tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGc                                   <  1:1346366/60‑1 (MQ=255)
                                             tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa                        >  1:785147/1‑71 (MQ=255)
                                              cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                       >  1:2503013/1‑71 (MQ=255)
                                               tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                         <  1:628252/52‑1 (MQ=255)
                                               tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTa                                         <  1:4870463/52‑1 (MQ=255)
                                                aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                       >  1:5509325/1‑69 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa                                       <  1:4227824/52‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa                        <  1:2149009/67‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAcc                      <  1:5054707/69‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                       <  1:4592101/68‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                       <  1:1877255/68‑1 (MQ=255)
                                                 aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                       <  1:1694742/68‑1 (MQ=255)
                                                 aCCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc                                               >  1:3547809/1‑44 (MQ=255)
                                                 aCCGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAAc                                               >  1:657043/1‑44 (MQ=255)
                                                  ccGCGATGAGTTCATCATGATCCGCAAGATGAAGAACGTTAACTGGCTGGATg                                     <  1:2007834/53‑1 (MQ=255)
                                                    gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                 <  1:3591986/71‑1 (MQ=255)
                                                     cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                 >  1:3728797/1‑70 (MQ=255)
                                                     cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTAc                <  1:2486915/71‑1 (MQ=255)
                                                      gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                 >  1:5679238/1‑69 (MQ=255)
                                                            tttGTTGTAGTGCGTAAGATGAAGAACATTGACTg                                             <  1:3443788/35‑1 (MQ=255)
                                                            tttGTTGTAGTGCGTAAGATGAAGAACATTGACTg                                             <  1:4366415/35‑1 (MQ=255)
                                                             ttgttgTAGTGCGTAAGATGAAGAACATTGACTGGTTAGa                                       <  1:1614334/40‑1 (MQ=255)
                                                              tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAg                                        >  1:786608/1‑38 (MQ=255)
                                                              tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAg                                        >  1:5434985/1‑38 (MQ=255)
                                                              tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt                                      >  1:4855383/1‑40 (MQ=255)
                                                              tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCaa                           >  1:4375949/1‑51 (MQ=255)
                                                              tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg       >  1:3439300/1‑71 (MQ=255)
                                                                  gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt                                      >  1:4466483/1‑36 (MQ=255)
                                                                  gtAGTGCGTAAGATGAAGAACATTGACTGGTTAGAt                                      >  1:3267547/1‑36 (MQ=255)
                                                                     gTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa                                >  1:4810196/1‑39 (MQ=255)
                                                                     |                                                                    
TATGCCCATCTGGCATACGGCTTTAACTACTATGGCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAA  >  Exported/835146‑835283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: