Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 835,249 G→A 9.4% G1236D (GGC→GAC) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported835,2490GA 9.4% 263.7 / 13.2 95G1236D (GGC→GAC) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base G (36/50);  new base A (2/7);  total (38/57)
Fisher's exact test for biased strand distribution p-value = 3.08e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.74e-01

Only 100 of 101 total aligned reads displayed.

GCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATC  >  Exported/835180‑835319
                                                                     |                                                                       
gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATgg                                                                         <  1:5209487/70‑1 (MQ=255)
gCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATgg                                                                         <  1:5495953/70‑1 (MQ=255)
 cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGTAGAACATTGACTGGTTAGATGGCg                                                                       <  1:2297451/71‑1 (MQ=255)
 cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                                                       <  1:2319062/71‑1 (MQ=255)
 cACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                                                       <  1:2292372/71‑1 (MQ=255)
  aCCGTAGGTTGTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                                                       <  1:4327716/70‑1 (MQ=255)
   ccGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCg                                                                       >  1:2935844/1‑69 (MQ=255)
     gTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAgg                                                                   >  1:932341/1‑71 (MQ=255)
         gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                                                               <  1:4239680/71‑1 (MQ=255)
          ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                                                                      <  1:1123275/63‑1 (MQ=255)
          ttCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGa                                                                      <  1:3286952/63‑1 (MQ=255)
           tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGc                                                                        <  1:1346366/60‑1 (MQ=255)
           tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGc                                                                        <  1:4522556/60‑1 (MQ=255)
           tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa                                                             >  1:785147/1‑71 (MQ=255)
            cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            >  1:2503013/1‑71 (MQ=255)
            cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            >  1:340032/1‑71 (MQ=255)
             tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            >  1:4231039/1‑70 (MQ=255)
              aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            >  1:5509325/1‑69 (MQ=255)
               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa                                                             <  1:2149009/67‑1 (MQ=255)
               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAcc                                                           <  1:5054707/69‑1 (MQ=255)
               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            <  1:1877255/68‑1 (MQ=255)
               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            <  1:4592101/68‑1 (MQ=255)
               aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                            <  1:1694742/68‑1 (MQ=255)
                  gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                      >  1:2143009/1‑71 (MQ=255)
                  gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                      <  1:3591986/71‑1 (MQ=255)
                   cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                      >  1:3728797/1‑70 (MQ=255)
                   cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTAc                                                     <  1:2486915/71‑1 (MQ=255)
                    gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                      >  1:5679238/1‑69 (MQ=255)
                            tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCaa                                                                >  1:4375949/1‑51 (MQ=255)
                            tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg                                            >  1:3439300/1‑71 (MQ=255)
                                 tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaaa                                      <  1:3577390/71‑1 (MQ=255)
                                   gTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGaa                                                                     >  1:4810196/1‑39 (MQ=255)
                                    tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAcc                                                           <  1:795173/48‑1 (MQ=255)
                                    tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATg                                    >  1:4763152/1‑70 (MQ=255)
                                    tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGa                                   <  1:2664627/71‑1 (MQ=255)
                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGgag                                                >  1:234486/1‑57 (MQ=255)
                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaaa                                      >  1:4523821/1‑66 (MQ=255)
                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaa                                  <  1:2785236/70‑1 (MQ=255)
                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaa                                  <  1:5539812/70‑1 (MQ=255)
                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaa                                  >  1:839110/1‑70 (MQ=255)
                                      cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaa                                  >  1:3055297/1‑70 (MQ=255)
                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa                                                          >  1:1141528/1‑45 (MQ=255)
                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAg                                                         >  1:4921519/1‑46 (MQ=255)
                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAgg                                                  >  1:3121267/1‑53 (MQ=255)
                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAgg                                                  >  1:3250143/1‑53 (MQ=255)
                                        tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAgg                                                  >  1:2007955/1‑53 (MQ=255)
                                        caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGaaa                                 <  1:2403104/68‑1 (MQ=255)
                                         aaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGAAAAt                               <  1:1966250/70‑1 (MQ=255)
                                          aGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAgg                                                  >  1:1118541/1‑51 (MQ=255)
                                            aTGAAGAACATTGACTGGTTAGATGGCGAAGGCaa                                                                >  1:2097997/1‑35 (MQ=255)
                                            aTGAAGAACATTGACTGGTTAGATGGCGAAGGCaa                                                                >  1:764503/1‑35 (MQ=255)
                                            aTGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGt                           >  1:3081228/1‑71 (MQ=255)
                                             tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAaaaaa                                     <  1:2527413/59‑2 (MQ=255)
                                             tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAaaaaa                                     <  1:5013240/59‑2 (MQ=255)
                                             tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAaaaaa                                     <  1:3227338/59‑2 (MQ=255)
                                             tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGtt                          <  1:4242196/71‑1 (MQ=255)
                                             tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGtt                          <  1:5153314/71‑1 (MQ=255)
                                             tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGt                           >  1:5310696/1‑70 (MQ=255)
                                              gaagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGtt                          >  1:2637404/1‑70 (MQ=255)
                                               aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTc                         <  1:4788100/70‑1 (MQ=255)
                                               aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTc                         <  1:4134869/70‑1 (MQ=255)
                                                agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGAAAATCCGTTcac                       <  1:2320362/71‑1 (MQ=255)
                                                agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGAAAATCCGTTca                        >  1:782842/1‑70 (MQ=255)
                                                agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcac                       <  1:4669113/71‑1 (MQ=255)
                                                agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcac                       <  1:3717937/71‑1 (MQ=255)
                                                agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTca                        >  1:3760167/1‑70 (MQ=255)
                                                 gaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGAAAATCCGTTcaca                      <  1:3837257/71‑1 (MQ=255)
                                                 gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg                                                   <  1:3545794/43‑1 (MQ=255)
                                                 gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcaca                      <  1:5561609/71‑1 (MQ=255)
                                                 gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcaca                      <  1:1974923/71‑1 (MQ=255)
                                                   aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaaaa                                >  1:3944474/1‑59 (MQ=255)
                                                   aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaaaa                                >  1:338585/1‑59 (MQ=255)
                                                   aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaaaa                                >  1:3805091/1‑59 (MQ=255)
                                                    cATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGt                   >  1:4031414/1‑71 (MQ=255)
                                                      ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga                                                 <  1:1278276/40‑1 (MQ=255)
                                                      ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATg                                    <  1:4468078/52‑1 (MQ=255)
                                                      ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATg                                    <  1:3000606/52‑1 (MQ=255)
                                                      ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATg                                    <  1:3338974/52‑1 (MQ=255)
                                                        gACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCgg                <  1:4125809/70‑1 (MQ=255)
                                                          cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCg                            >  1:4727742/1‑56 (MQ=255)
                                                          cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCg                            >  1:5575438/1‑56 (MQ=255)
                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCg                            <  1:5532706/54‑1 (MQ=255)
                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACaa                     <  1:4914260/61‑1 (MQ=255)
                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACaa                     <  1:1517586/61‑1 (MQ=255)
                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACaa                     <  1:4332111/61‑1 (MQ=255)
                                                            ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACaa                     <  1:5350169/61‑1 (MQ=255)
                                                             gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATg            <  1:5165659/69‑1 (MQ=255)
                                                              ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTg         <  1:3027281/71‑1 (MQ=255)
                                                              ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTg         <  1:3361905/71‑1 (MQ=255)
                                                                aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaaa                                      <  1:2638800/40‑1 (MQ=255)
                                                                aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaaa                                      <  1:2327121/40‑1 (MQ=255)
                                                                aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc        <  1:944331/70‑1 (MQ=255)
                                                                 gATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt       <  1:298726/70‑1 (MQ=255)
                                                                 gATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt       >  1:3769060/1‑70 (MQ=255)
                                                                 gATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg      <  1:236991/71‑1 (MQ=255)
                                                                  aTGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa     <  1:2242913/71‑1 (MQ=255)
                                                                    ggCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaaaa                                >  1:4570726/1‑42 (MQ=255)
                                                                    ggCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcaca                      >  1:2531585/1‑52 (MQ=255)
                                                                    ggCGAAGGCAATGACAAGGTACAGGAGAGCGTAAAATGaaaa                                >  1:4590345/1‑42 (MQ=255)
                                                                     gCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc  <  1:1585040/71‑1 (MQ=255)
                                                                     |                                                                       
GCACCGTAGGTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATC  >  Exported/835180‑835319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: