Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 835,259 T→C 8.7% N1239N (AAT→AAC) ‡ narG → nitrate reductase 1, alpha subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported835,2590TC 8.7% 284.9 / 13.4 103N1239N (AAT→AAC) ‡narGnitrate reductase 1, alpha subunit
Reads supporting (aligned to +/- strand):  ref base T (43/51);  new base C (2/7);  total (45/58)
Fisher's exact test for biased strand distribution p-value = 2.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 105 total aligned reads displayed.

GTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAG  >  Exported/835189‑835327
                                                                      |                                                                     
gTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAAt                                                                       <  1:4239680/71‑1 (MQ=255)
  tCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa                                                                     >  1:785147/1‑71 (MQ=255)
   cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                                    >  1:2503013/1‑71 (MQ=255)
   cTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                                    >  1:340032/1‑71 (MQ=255)
    tAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                                    >  1:4231039/1‑70 (MQ=255)
     aaCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                                    >  1:5509325/1‑69 (MQ=255)
      aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGa                                                                     <  1:2149009/67‑1 (MQ=255)
      aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                                    <  1:1694742/68‑1 (MQ=255)
      aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                                    <  1:1877255/68‑1 (MQ=255)
      aCCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAc                                                                    <  1:4592101/68‑1 (MQ=255)
         gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                              <  1:3591986/71‑1 (MQ=255)
         gcgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                              >  1:2143009/1‑71 (MQ=255)
          cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                              >  1:3728797/1‑70 (MQ=255)
          cgATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTAc                                                             <  1:2486915/71‑1 (MQ=255)
           gATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTa                                                              >  1:5679238/1‑69 (MQ=255)
                   tgttgtAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCg                                                    >  1:3439300/1‑71 (MQ=255)
                        tAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaaa                                              <  1:3577390/71‑1 (MQ=255)
                           tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGAcc                                                                   <  1:795173/48‑1 (MQ=255)
                           tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATg                                            >  1:4763152/1‑70 (MQ=255)
                           tGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGa                                           <  1:2664627/71‑1 (MQ=255)
                             cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaaa                                              >  1:4523821/1‑66 (MQ=255)
                             cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaa                                          >  1:3055297/1‑70 (MQ=255)
                             cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaa                                          <  1:5539812/70‑1 (MQ=255)
                             cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaa                                          <  1:2785236/70‑1 (MQ=255)
                             cGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaa                                          >  1:839110/1‑70 (MQ=255)
                               tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCa                                                                  >  1:1141528/1‑45 (MQ=255)
                               tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAg                                                                 >  1:4921519/1‑46 (MQ=255)
                               tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAgg                                                          >  1:3121267/1‑53 (MQ=255)
                               tAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAgg                                                          >  1:2007955/1‑53 (MQ=255)
                               caaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGaaa                                         <  1:2403104/68‑1 (MQ=255)
                                aaGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGAAAAt                                       <  1:1966250/70‑1 (MQ=255)
                                 aGATGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAgg                                                          >  1:1118541/1‑51 (MQ=255)
                                   aTGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGt                                   >  1:3081228/1‑71 (MQ=255)
                                    tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAaaaaa                                             <  1:3227338/59‑2 (MQ=255)
                                    tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAaaaaa                                             <  1:5013240/59‑2 (MQ=255)
                                    tGAAGAACGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAaaaaa                                             <  1:2527413/59‑2 (MQ=255)
                                    tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGtt                                  <  1:4242196/71‑1 (MQ=255)
                                    tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGtt                                  <  1:5153314/71‑1 (MQ=255)
                                    tGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGt                                   >  1:5310696/1‑70 (MQ=255)
                                     gaagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGtt                                  >  1:2637404/1‑70 (MQ=255)
                                      aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTc                                 <  1:4134869/70‑1 (MQ=255)
                                      aagaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTc                                 <  1:4788100/70‑1 (MQ=255)
                                       agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGAAAATCCGTTcac                               <  1:2320362/71‑1 (MQ=255)
                                       agaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGAAAATCCGTTca                                >  1:782842/1‑70 (MQ=255)
                                       agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcac                               <  1:3717937/71‑1 (MQ=255)
                                       agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcac                               <  1:4669113/71‑1 (MQ=255)
                                       agaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTca                                >  1:3760167/1‑70 (MQ=255)
                                        gaaCGTTAACTGGCTGGATGATGAAGGTCGCGATCAGGTACAGGAGGCGAAAAAATGAAAATCCGTTcaca                              <  1:3837257/71‑1 (MQ=255)
                                        gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAg                                                           <  1:3545794/43‑1 (MQ=255)
                                        gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcaca                              <  1:5561609/71‑1 (MQ=255)
                                        gaaCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcaca                              <  1:1974923/71‑1 (MQ=255)
                                          aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaaaa                                        >  1:338585/1‑59 (MQ=255)
                                          aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaaaa                                        >  1:3805091/1‑59 (MQ=255)
                                          aCATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaaaa                                        >  1:3944474/1‑59 (MQ=255)
                                           cATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGt                           >  1:4031414/1‑71 (MQ=255)
                                             ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGga                                                         <  1:1278276/40‑1 (MQ=255)
                                             ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATg                                            <  1:3338974/52‑1 (MQ=255)
                                             ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATg                                            <  1:4468078/52‑1 (MQ=255)
                                             ttGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATg                                            <  1:3000606/52‑1 (MQ=255)
                                                 cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCg                                    >  1:4727742/1‑56 (MQ=255)
                                                 cTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCg                                    >  1:5575438/1‑56 (MQ=255)
                                                   ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCg                                    <  1:5532706/54‑1 (MQ=255)
                                                   ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACaa                             <  1:4332111/61‑1 (MQ=255)
                                                   ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACaa                             <  1:1517586/61‑1 (MQ=255)
                                                   ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACaa                             <  1:5350169/61‑1 (MQ=255)
                                                   ggTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACaa                             <  1:4914260/61‑1 (MQ=255)
                                                    gTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATg                    <  1:5165659/69‑1 (MQ=255)
                                                     ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTg                 <  1:3027281/71‑1 (MQ=255)
                                                     ttAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTg                 <  1:3361905/71‑1 (MQ=255)
                                                       aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaaa                                              <  1:2327121/40‑1 (MQ=255)
                                                       aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTaaaa                                              <  1:2638800/40‑1 (MQ=255)
                                                       aGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGc                <  1:944331/70‑1 (MQ=255)
                                                        gATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt               >  1:3769060/1‑70 (MQ=255)
                                                        gATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCt               <  1:298726/70‑1 (MQ=255)
                                                        gATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTg              <  1:236991/71‑1 (MQ=255)
                                                         aTGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGa             <  1:2242913/71‑1 (MQ=255)
                                                           ggCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGaaaa                                        >  1:4570726/1‑42 (MQ=255)
                                                           ggCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcaca                              >  1:2531585/1‑52 (MQ=255)
                                                           ggCGAAGGCAATGACAAGGTACAGGAGAGCGTAAAATGaaaa                                        >  1:4590345/1‑42 (MQ=255)
                                                            gCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAAtc          <  1:1585040/71‑1 (MQ=255)
                                                              gAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATTGTGCTGAAtctc        <  1:5349937/71‑1 (MQ=255)
                                                               aaGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCg       <  1:3902747/71‑1 (MQ=255)
                                                                aGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGa      >  1:5371629/1‑71 (MQ=255)
                                                                 ggCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt     <  1:4381332/71‑1 (MQ=255)
                                                                  gCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcac                               <  1:5511454/44‑1 (MQ=255)
                                                                  gCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTcac                               <  1:1772219/44‑1 (MQ=255)
                                                                  gCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACaa                             <  1:1976999/46‑1 (MQ=255)
                                                                  gCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt     <  1:4920049/70‑1 (MQ=255)
                                                                   cAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATaa   <  1:3129607/71‑1 (MQ=255)
                                                                    aaTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGtt                                  >  1:1938330/1‑39 (MQ=255)
                                                                    aaTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGtt                                  >  1:3066947/1‑39 (MQ=255)
                                                                    aaTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGtt                                  >  1:3968704/1‑39 (MQ=255)
                                                                    aaTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGt                                   >  1:2836342/1‑38 (MQ=255)
                                                                    aaTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGAt     >  1:178519/1‑68 (MQ=255)
                                                                    aaTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg  >  1:4002944/1‑71 (MQ=255)
                                                                    aaTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg  >  1:4613436/1‑71 (MQ=255)
                                                                    aaTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGGGCTGAATCTCGATaa   <  1:5613257/70‑1 (MQ=255)
                                                                     aTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCgg                        <  1:771026/48‑1 (MQ=255)
                                                                     aTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg  >  1:385532/1‑70 (MQ=255)
                                                                     aTGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAg  >  1:1670486/1‑70 (MQ=255)
                                                                      |                                                                     
GTTCTAACCGCGATGAGTTTGTTGTAGTGCGTAAGATGAAGAACATTGACTGGTTAGATGGCGAAGGCAATGACCAGGTACAGGAGAGCGTAAAATGAAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAG  >  Exported/835189‑835327

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: