| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 835,354 | T→G | 44.0% | V24V (GTT→GTG) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 835,354 | 0 | T | G | 44.0% | 4.4 / 187.4 | 132 | V24V (GTT→GTG) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (28/46); new base G (29/29); total (57/75) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
AAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAG > Exported/835286‑835423 | aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTTCCACACCTGTTCAGTTAc > 1:1753273/1‑71 (MQ=255)aaaaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAc > 1:2988737/1‑71 (MQ=255) aaaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGAcc > 1:3033300/1‑71 (MQ=255) aaTTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTAcc < 1:5463800/70‑1 (MQ=255) aaTCCGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCt < 1:3422085/71‑1 (MQ=255) tccGTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCt > 1:2955088/3‑69 (MQ=255) gTTCACAAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaa < 1:39445/71‑1 (MQ=255) gTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaa < 1:4627365/70‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTTTaaaa < 1:1391440/71‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaaaa < 1:823694/71‑1 (MQ=255) ttCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTaaa < 1:2080557/70‑1 (MQ=255) cacaAGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAAc > 1:2548851/1‑71 (MQ=255) aaGTCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaaa > 1:5685662/1‑66 (MQ=255) tCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTgg < 1:5621635/71‑1 (MQ=255) tCGGCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTg < 1:4548543/70‑1 (MQ=255) ggCATGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGAc < 1:4918992/71‑1 (MQ=255) aTGGTGCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGAccg < 1:401755/70‑2 (MQ=255) gTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCg < 1:696788/71‑1 (MQ=255) gCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTa > 1:921225/1‑50 (MQ=255) gCTTAACCTCGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTa > 1:2454457/1‑50 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4773351/1‑70 (MQ=255) gCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4406792/1‑70 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg > 1:1232917/1‑70 (MQ=255) aaTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc > 1:290336/1‑51 (MQ=255) aaTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc > 1:2485647/1‑51 (MQ=255) aaTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc > 1:557873/1‑51 (MQ=255) acctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAgg < 1:1544852/68‑1 (MQ=255) aTCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTTAAGGc < 1:2791370/71‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCt > 1:1289996/1‑41 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGt < 1:4109947/51‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGt < 1:3492849/51‑1 (MQ=255) tctcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGt < 1:2729899/51‑1 (MQ=255) cctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGca < 1:3393405/70‑2 (MQ=255) cctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGca < 1:1450542/70‑2 (MQ=255) ctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGcat < 1:3733652/71‑3 (MQ=255) tcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTa > 1:690910/1‑36 (MQ=255) tcGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTgg > 1:511548/1‑53 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTaaa < 1:1253319/44‑1 (MQ=16) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAg > 1:4838527/1‑66 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGc < 1:1833308/68‑1 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGc < 1:1188328/68‑1 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGc < 1:4446910/68‑1 (MQ=255) cGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTg > 1:4888898/1‑51 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTgg > 1:5387749/1‑70 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTgg < 1:2055536/70‑1 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTgg < 1:757757/70‑1 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTgg > 1:2555624/1‑70 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGCACCAGCCGTGAAGGCGTgg < 1:4789397/70‑1 (MQ=255) aTAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc < 1:3557967/44‑1 (MQ=255) aTAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc < 1:2326283/44‑1 (MQ=255) aTAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAAc < 1:2184372/44‑1 (MQ=255) tAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACAAGCCGTGAAGGCGTGGAat < 1:5430758/71‑1 (MQ=255) gTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATg < 1:1222241/71‑1 (MQ=255) tGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGc < 1:2175775/71‑1 (MQ=255) gtaTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGt > 1:1063941/3‑40 (MQ=25) gtaTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGt > 1:4808949/3‑40 (MQ=25) gtaTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGt > 1:5276277/3‑40 (MQ=25) gtaTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGt > 1:2341640/3‑40 (MQ=25) taTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGcat > 1:4991255/2‑69 (MQ=255) aTCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg < 1:4955137/71‑1 (MQ=255) aTCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg < 1:3262564/71‑1 (MQ=255) tCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAgg < 1:4789916/55‑1 (MQ=255) tCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg < 1:3297691/51‑1 (MQ=255) tCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTgg < 1:285264/71‑1 (MQ=255) tCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg > 1:3219286/1‑70 (MQ=255) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTg > 1:468651/1‑38 (MQ=25) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTg > 1:4856746/1‑38 (MQ=25) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGtt < 1:1970209/71‑1 (MQ=255) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGt < 1:5213276/70‑1 (MQ=255) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGt < 1:2100921/70‑1 (MQ=255) cTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTca < 1:1467275/71‑1 (MQ=255) ccATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaaca < 1:814686/71‑1 (MQ=255) ccATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaaca < 1:2643060/71‑1 (MQ=255) ccACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaac < 1:471589/70‑1 (MQ=255) catacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaac < 1:3604901/66‑1 (MQ=255) catacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaac < 1:4192014/66‑1 (MQ=255) cacaCCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaacaa < 1:2106645/71‑1 (MQ=255) cacaCCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaacaa < 1:3044269/71‑1 (MQ=255) tacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGag < 1:2068490/51‑2 (MQ=255) tacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGag < 1:170916/51‑2 (MQ=255) tacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGag < 1:5557165/51‑2 (MQ=255) cacCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACg > 1:4128877/1‑71 (MQ=255) acCTGTTCGGCGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGttta < 1:4527608/64‑3 (MQ=255) acCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAat < 1:2963414/52‑1 (MQ=255) acCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGt > 1:5084967/1‑71 (MQ=255) ccTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGca > 1:3193917/1‑55 (MQ=255) ccTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGtc > 1:3684700/1‑70 (MQ=255) ccTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGtc > 1:57590/1‑70 (MQ=255) ccTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg < 1:2062366/39‑1 (MQ=255) cTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACTCATGGTTTAACAACGtc > 1:1973877/1‑69 (MQ=255) gTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGag < 1:2268169/47‑2 (MQ=255) ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaa > 1:1289810/1‑71 (MQ=255) ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCg > 1:5080133/1‑41 (MQ=255) ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata > 1:4977295/1‑48 (MQ=255) ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa < 1:1053184/69‑1 (MQ=255) tCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGaa < 1:963621/45‑1 (MQ=255) cAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTc > 1:4228091/1‑56 (MQ=255) ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAg > 1:3842573/1‑71 (MQ=255) ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAg > 1:5082360/1‑71 (MQ=255) tACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCg < 1:871703/35‑1 (MQ=255) | AAAATTCGTTCACAAGTCGGCATGGTGCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAG > Exported/835286‑835423 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |