| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 835,381 | T→C | 38.3% | R33R (CGT→CGC) | narH → | nitrate reductase 1, beta (Fe‑S) subunit |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 835,381 | 0 | T | C | 38.3% | 93.4 / 130.5 | 128 | R33R (CGT→CGC) | narH | nitrate reductase 1, beta (Fe‑S) subunit |
| Reads supporting (aligned to +/- strand): ref base T (29/50); new base C (22/27); total (51/77) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.58e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.24e-01 | |||||||||||
Only 100 of 132 total aligned reads displayed.
GCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGG > Exported/835312‑835450 | gCTGAATCTCGATAAGTGCATCGGCTGCCACAGCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:875915/1‑70 (MQ=255)gCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4773351/1‑70 (MQ=255)gCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGt > 1:4406792/1‑70 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg < 1:4804319/70‑1 (MQ=255) cTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGa < 1:2550486/71‑1 (MQ=255) acctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAgg < 1:1544852/68‑1 (MQ=255) cctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGca < 1:3393405/70‑2 (MQ=255) ctcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGcat < 1:3733652/71‑3 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGc < 1:4108525/68‑1 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGc < 1:1188328/68‑1 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGc < 1:4446910/68‑1 (MQ=255) tcGACAAATGTATCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGc < 1:1833308/68‑1 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTgg > 1:5387749/1‑70 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTgg < 1:757757/70‑1 (MQ=255) gATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGCACCAGCCGTGAAGGCGTgg < 1:4789397/70‑1 (MQ=255) aGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata < 1:5479613/70‑1 (MQ=255) gTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATg < 1:1222241/71‑1 (MQ=255) tgtaTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGa > 1:1399268/4‑65 (MQ=255) tGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGc < 1:2175775/71‑1 (MQ=255) taTCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGcat > 1:4991255/2‑69 (MQ=255) aTCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg < 1:4955137/71‑1 (MQ=255) aTCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg < 1:3262564/71‑1 (MQ=255) tCGGCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAgg < 1:4789916/55‑1 (MQ=255) tCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTgg < 1:285264/71‑1 (MQ=255) tCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTg > 1:3219286/1‑70 (MQ=255) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGaa > 1:3579997/1‑51 (MQ=255) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGtt < 1:1970209/71‑1 (MQ=255) ggCTGCCATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGt < 1:5213276/70‑1 (MQ=255) ggCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGaa > 1:2987253/1‑51 (MQ=255) ggCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGt > 1:2009209/1‑70 (MQ=255) cTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTca < 1:4868144/71‑1 (MQ=255) cTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTca < 1:1467275/71‑1 (MQ=255) ccATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaaca < 1:2643060/71‑1 (MQ=255) ccATACCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaaca < 1:814686/71‑1 (MQ=255) catacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaac < 1:3604901/66‑1 (MQ=255) catacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaac < 1:4192014/66‑1 (MQ=255) cacaCCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaacaa < 1:2106645/71‑1 (MQ=255) cacaCCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaacaa < 1:2951129/71‑1 (MQ=255) cacaCCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaacaa < 1:3044269/71‑1 (MQ=255) tacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGag < 1:5557165/51‑2 (MQ=255) tacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGag < 1:2068490/51‑2 (MQ=255) tacCTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGag < 1:170916/51‑2 (MQ=255) cacCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACg < 1:5591519/71‑1 (MQ=255) cacCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACg > 1:4128877/1‑71 (MQ=255) acCTGTTCGGCGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGttta < 1:4527608/64‑3 (MQ=255) acCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAat < 1:2963414/52‑1 (MQ=255) acCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGt > 1:5084967/1‑71 (MQ=255) ccTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAgta > 1:5330937/1‑52 (MQ=255) ccTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGtc > 1:57590/1‑70 (MQ=255) ccTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTg < 1:2062366/39‑1 (MQ=255) cTGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACTCATGGTTTAACAACGtc > 1:1973877/1‑69 (MQ=255) tGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGaa > 1:473428/1‑39 (MQ=16) tGTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGaa > 1:3467012/1‑39 (MQ=16) gTTCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGag < 1:2268169/47‑2 (MQ=255) ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaa > 1:1289810/1‑71 (MQ=255) ttCGGTGACCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaa > 1:2403902/1‑71 (MQ=255) ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCg > 1:5080133/1‑41 (MQ=255) ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata > 1:4977295/1‑48 (MQ=255) ttCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa < 1:1053184/69‑1 (MQ=255) cAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTc > 1:4228091/1‑56 (MQ=255) cAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTc > 1:2077286/1‑56 (MQ=255) gtgaCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCaa < 1:2572197/68‑1 (MQ=255) gtgaCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCaa < 1:3125305/68‑1 (MQ=255) ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAg > 1:5082360/1‑71 (MQ=255) ttACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAg > 1:3842573/1‑71 (MQ=255) tACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCg < 1:871703/35‑1 (MQ=255) gaCCTGTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCaaac > 1:3144337/2‑69 (MQ=255) aCCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaac > 1:1615938/1‑54 (MQ=255) ccTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCa < 1:314561/66‑1 (MQ=255) gTAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTtaac < 1:128933/50‑1 (MQ=255) gTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaaca < 1:1812442/51‑1 (MQ=255) gTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTcaaca < 1:4768250/51‑1 (MQ=255) gTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa > 1:1070581/1‑58 (MQ=255) gTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa > 1:2549367/1‑58 (MQ=255) gTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCggg > 1:65983/1‑70 (MQ=255) tAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATg < 1:353592/42‑1 (MQ=255) tAAAAACGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCggg < 1:5453935/69‑1 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata > 1:5130005/1‑36 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAata > 1:5629587/1‑36 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa < 1:2172006/57‑1 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGa < 1:2614785/57‑1 (MQ=255) tAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGc < 1:5391119/70‑1 (MQ=255) aaaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCaacaac > 1:3419345/1‑51 (MQ=255) aaaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGc > 1:763680/1‑69 (MQ=255) aaaaCGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGAAACCAAACCGGGcatt > 1:3831433/1‑68 (MQ=255) aaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCaa > 1:4597067/1‑61 (MQ=255) aaaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCaa > 1:2311803/1‑61 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTg < 1:3669423/52‑1 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTg < 1:3225637/52‑1 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTg < 1:2925466/52‑1 (MQ=255) aaaCGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCa < 1:1014431/69‑1 (MQ=255) aCGTCTGGACCGGGCGCGAAGGCATGGAGTACGCATGGTTTAACAACGTCGaaa > 1:1029114/1‑54 (MQ=255) gTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCtt > 1:1468629/1‑71 (MQ=255) tGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTccc > 1:2553376/1‑71 (MQ=255) gACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTccc < 1:116334/69‑1 (MQ=255) gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgac < 1:2235549/71‑1 (MQ=255) gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgac < 1:5170359/71‑1 (MQ=255) gCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgac < 1:4536268/71‑1 (MQ=255) ccGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCgactgact < 1:2013259/71‑1 (MQ=255) gTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTgg < 1:1987430/71‑1 (MQ=255) | GCTGAATCTCGATAAGTGCATCGGCTGCCACACCTGTTCAGTTACCTGTAAAAACGTCTGGACCAGCCGTGAAGGCGTGGAATATGCGTGGTTCAACAACGTGGAAACCAAGCCGGGCCAGGGCTTCCCGACTGACTGG > Exported/835312‑835450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |