Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 836,481 A→G 18.4% K400R (AAA→AGA) ‡ narH → nitrate reductase 1, beta (Fe‑S) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported836,4810AG18.4% 131.9 / 23.7 65K400R (AAA→AGA) ‡narHnitrate reductase 1, beta (Fe‑S) subunit
Reads supporting (aligned to +/- strand):  ref base A (30/23);  new base G (7/5);  total (37/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.98e-01

TGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAC  >  Exported/836412‑836548
                                                                     |                                                                   
tGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCaaa                                                                    >  1:2621459/1‑71 (MQ=255)
    aGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCgg                                                                <  1:2195677/71‑1 (MQ=255)
     gACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGt                                                               <  1:386904/71‑1 (MQ=255)
        gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt                                                            >  1:2737099/1‑71 (MQ=255)
        gTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt                                                            >  1:2060746/1‑71 (MQ=255)
          cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc                                                          >  1:3698793/1‑71 (MQ=255)
          cGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgc                                                          >  1:3473738/1‑71 (MQ=255)
           gAAAGCCTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTa                                                              >  1:1249682/1‑66 (MQ=255)
              aGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTgcgcg                                                       >  1:1105263/1‑70 (MQ=255)
                 cTGCGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt                                                   <  1:2254244/71‑1 (MQ=255)
                 cTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACt                                                   <  1:2687358/71‑1 (MQ=255)
                    cGTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGa                                                 <  1:4432431/70‑1 (MQ=255)
                    cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaa                                                <  1:4110428/71‑1 (MQ=255)
                    cGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                                 >  1:3630906/1‑70 (MQ=255)
                     gTATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGaa                                                >  1:1837881/1‑70 (MQ=255)
                      tATTCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCt                                                   <  1:1188187/66‑1 (MQ=255)
                      tATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACt                                                            <  1:1475079/57‑1 (MQ=255)
                        ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg                                             >  1:401318/1‑70 (MQ=255)
                        ttCCGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACg                                             <  1:1596027/70‑1 (MQ=255)
                         tCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa                                           >  1:65595/1‑71 (MQ=255)
                          ccGGTGCAATATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGAt                                          >  1:1989129/1‑71 (MQ=255)
                          ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt                                          >  1:1161640/1‑71 (MQ=255)
                          ccGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTAt                                          >  1:4710526/1‑71 (MQ=255)
                             gTACAGTATCTGGCGAATCTGCTGACCGCCGGAGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt                                       <  1:537934/71‑1 (MQ=255)
                               aCAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCt                                       <  1:1362446/69‑1 (MQ=255)
                                caatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc                                    >  1:982427/4‑71 (MQ=255)
                                 aatATCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGc                                    >  1:5160913/3‑70 (MQ=255)
                                  gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa                                                     >  1:2672883/1‑52 (MQ=255)
                                  gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa                                                     >  1:4812093/1‑52 (MQ=255)
                                  gTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCa                                                     >  1:603611/1‑52 (MQ=255)
                                   tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc                                                                   <  1:3942951/37‑1 (MQ=255)
                                   tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc                                                                   <  1:4700042/37‑1 (MQ=255)
                                   tATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTa                                                              >  1:5315952/1‑42 (MQ=255)
                                    aTCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                                 >  1:236465/1‑54 (MQ=255)
                                    aTCTCGCCAATATGTTGAGTGCCGGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATg                                >  1:295549/1‑71 (MQ=255)
                                     tCTGGCGAATCTGCTGACCGCCGGTGATACCAAAc                                                                   <  1:1542139/35‑1 (MQ=255)
                                       tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                                 <  1:1812489/51‑1 (MQ=255)
                                       tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                                 <  1:1838486/51‑1 (MQ=255)
                                       tGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                                 <  1:727777/51‑1 (MQ=255)
                                            aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGaaa                                               >  1:5265714/1‑48 (MQ=255)
                                            aaTCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                                 <  1:1351984/46‑1 (MQ=255)
                                                tgctgACCGCCGGTGATACCAAACCGGTACTGCTCGCACTGa                                                 <  1:4878885/42‑1 (MQ=255)
                                                tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                                 <  1:4524877/42‑1 (MQ=255)
                                                tgctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGa                                                 <  1:2862653/42‑1 (MQ=255)
                                                  ctgACCGCCGGTTATACCAAACCGGTACTGCGCGCACTGAAACGTa                                           >  1:4896566/1‑46 (MQ=255)
                                                  ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTa                                           >  1:5451852/1‑46 (MQ=255)
                                                  ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAAc                   <  1:3179718/70‑1 (MQ=255)
                                                  ctgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACg                  >  1:563775/1‑71 (MQ=255)
                                                   tgACCGCCGGTGATACCAAACCGGTACTGCGTGCACTGAAACGTATGCTGGCGATGCGTCAt                          >  1:2935420/1‑62 (MQ=255)
                                                   tgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCAt                          >  1:1198279/1‑62 (MQ=255)
                                                   tgACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCAt                          >  1:2774006/1‑62 (MQ=255)
                                                       cgccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCt              >  1:3694536/1‑70 (MQ=255)
                                                         ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACg                                             <  1:4073761/37‑1 (MQ=255)
                                                         ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACg                                             <  1:1461203/37‑1 (MQ=255)
                                                         ccgGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTg                                      <  1:1610253/44‑1 (MQ=255)
                                                          cgGCGATACCGGTCCGGTACTGCGGGCGCTGAAACGGATGATGGCGATGCGCCact                         <  1:1111407/56‑3 (MQ=255)
                                                            gTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAAcc        >  1:5142085/1‑71 (MQ=255)
                                                             tGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTCGCGATGCGTCATTACAAACGTGCTGAAACCg       <  1:4679175/71‑1 (MQ=255)
                                                              gATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGt      >  1:3463473/1‑71 (MQ=255)
                                                              gATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGt      >  1:2490242/1‑71 (MQ=255)
                                                               aTACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGGGATGCGTCATTACAAACGTGCTGAAACCGtt     <  1:4286664/71‑1 (MQ=255)
                                                                 aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGa                                  >  1:1714345/1‑40 (MQ=255)
                                                                 aCCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa   >  1:200883/1‑71 (MQ=255)
                                                                  ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc  >  1:5016305/1‑71 (MQ=255)
                                                                  ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc  >  1:4891903/1‑71 (MQ=255)
                                                                  ccAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAc  >  1:3452521/1‑71 (MQ=255)
                                                                    aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCTTTACaaa                    >  1:1548044/1‑51 (MQ=255)
                                                                    aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa   <  1:20819/68‑1 (MQ=255)
                                                                    aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa   <  1:2536256/68‑1 (MQ=255)
                                                                    aaaCCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGa   >  1:4140248/1‑68 (MQ=255)
                                                                     |                                                                   
TGCCAGACGTCGAAAGCCTGCGTATTCCGGTACAGTATCTGGCGAATCTGCTGACCGCCGGTGATACCAAACCGGTACTGCGCGCACTGAAACGTATGCTGGCGATGCGTCATTACAAACGTGCTGAAACCGTTGAC  >  Exported/836412‑836548

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: