Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 837,087 G→A 40.3% A90A (GCG→GCA narJ → molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported837,0870GA40.3% 49.4 / 79.6 77A90A (GCG→GCAnarJmolybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1
Reads supporting (aligned to +/- strand):  ref base G (20/26);  new base A (17/14);  total (37/40)
Fisher's exact test for biased strand distribution p-value = 3.60e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

GTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATC  >  Exported/837020‑837157
                                                                   |                                                                      
gTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtg                                                                     <  1:3691372/71‑1 (MQ=255)
   gCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggt                                                                   <  1:1477056/70‑1 (MQ=255)
    gcgcaCCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtgg                                                                 <  1:2844976/70‑1 (MQ=255)
    gcgcaCCACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtgg                                                                 <  1:4929275/70‑1 (MQ=255)
         ccACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACct                                                             >  1:5264352/1‑70 (MQ=255)
          cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtgttgg                                                                 <  1:5638980/65‑1 (MQ=255)
          cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtgg                                                                 <  1:4664664/65‑1 (MQ=255)
          cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtgg                                                                    >  1:3892172/1‑62 (MQ=255)
          cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc                                                           >  1:3472542/1‑71 (MQ=255)
          cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc                                                           >  1:2444782/1‑71 (MQ=255)
          cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc                                                           >  1:4472078/1‑71 (MQ=255)
          cACGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc                                                           >  1:4174436/1‑71 (MQ=255)
          cACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg                                                                 <  1:3683966/65‑1 (MQ=255)
            cGTCGCTGCTGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgctg                                                         >  1:3029830/1‑71 (MQ=255)
              tcgctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgc                                                       <  1:1388791/68‑1 (MQ=255)
               cgctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcg                                                      >  1:4414028/3‑71 (MQ=255)
                gctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtg                                                                     >  1:3958707/2‑55 (MQ=255)
                gctgctgctgTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcg                                                      <  1:1021282/69‑1 (MQ=255)
                gctgctgctgTTCGAACATATTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcg                                                      <  1:3803419/69‑1 (MQ=255)
                aCTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg                                                      >  1:4511865/1‑70 (MQ=255)
                 cTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGgcg                                                      >  1:955244/1‑69 (MQ=255)
                  tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg                                                                 <  1:4333687/57‑1 (MQ=255)
                  tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtggtgg                                                                 <  1:352813/57‑1 (MQ=255)
                  tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAg                                                   >  1:2359348/1‑71 (MQ=255)
                   gctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAg                                                   >  1:2021444/3‑70 (MQ=255)
                    ttGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGAtg                                                                     >  1:1548587/1‑51 (MQ=255)
                    ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc                                                           <  1:3437199/60‑1 (MQ=255)
                    ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGt                                                  <  1:4773093/69‑1 (MQ=255)
                    ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTa                                                 <  1:1249391/70‑1 (MQ=255)
                    ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTa                                                 >  1:2197833/2‑71 (MQ=255)
                     tGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTgg                                                        >  1:2438912/1‑63 (MQ=255)
                       cTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACg                                               <  1:241880/70‑1 (MQ=255)
                       cTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGa                                              <  1:1022181/71‑1 (MQ=255)
                       cTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGa                                              <  1:229261/71‑1 (MQ=255)
                       cTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGa                                              <  1:2408335/71‑1 (MQ=255)
                         gTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGag                                             >  1:386382/1‑70 (MQ=255)
                         gTTTGAACATGTGCACGGCGAATCCCGCGAC‑‑CGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagcag                                          >  1:2686838/1‑71 (MQ=255)
                          tttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctg                                                            <  1:2986800/54‑1 (MQ=255)
                          tttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGag                                             <  1:2469044/69‑1 (MQ=255)
                           ttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGa                                                                       >  1:4370747/1‑42 (MQ=255)
                           ttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagca                                           <  1:600224/70‑1 (MQ=255)
                            tGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagcag                                          <  1:2064394/70‑1 (MQ=255)
                            cgAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAAtggtg                                                                  >  1:5423722/2‑46 (MQ=16)
                             gAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGa                                              <  1:2838369/65‑1 (MQ=255)
                               tcATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctgc                                                           >  1:719290/2‑50 (MQ=255)
                               aCATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc                                                           >  1:456750/1‑50 (MQ=255)
                               aCATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctgc                                                           >  1:4525617/1‑50 (MQ=255)
                               aCATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACctg                                                            >  1:2669587/1‑49 (MQ=255)
                               aCATGTTCATGCCGAGTCCAGCGATCGCGGCCAGGCAATGGTGGACctgc                                                           >  1:5371076/1‑50 (MQ=255)
                               aCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctgc                                                           >  1:644956/1‑50 (MQ=255)
                               aCATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACctgc                                                           >  1:848913/1‑50 (MQ=255)
                                 aTGTGCACGGCGAATCCCGCGACCCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAgcagca                                        <  1:4636300/66‑1 (MQ=255)
                                    tgCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTg                                 <  1:2243148/71‑1 (MQ=255)
                                      cACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGc                                <  1:2103049/70‑1 (MQ=255)
                                         ggCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGt                             >  1:3496941/1‑70 (MQ=255)
                                         ggCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGt                             >  1:5424827/1‑70 (MQ=255)
                                         ggCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACATGCTGGCGCAGTACGAGCAGCACGGCTTGCAGt                             >  1:5243615/1‑70 (MQ=255)
                                          gCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTa                           <  1:4200296/71‑1 (MQ=255)
                                          ccGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGAAAAGGTCGGCCTGCAgct                            <  1:4813993/69‑3 (MQ=255)
                                           cGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGAAAAGGTCGGCCTGCAgctg                           >  1:1174080/1‑67 (MQ=255)
                                               tCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGgcgg                                                     <  1:4534355/40‑2 (MQ=37)
                                                cccGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGc                     >  1:5614802/1‑71 (MQ=255)
                                                  cgcgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcg                   <  1:219901/71‑1 (MQ=255)
                                                  cgcgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcg                   >  1:1229473/1‑71 (MQ=255)
                                                   gcgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcgc                  >  1:4320596/1‑71 (MQ=255)
                                                   gcgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcg                   >  1:2388511/1‑70 (MQ=255)
                                                    cgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcgcg                 <  1:1779828/71‑1 (MQ=255)
                                                     gACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGa                <  1:284705/71‑1 (MQ=255)
                                                     gACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGa                <  1:2819522/71‑1 (MQ=255)
                                                       ccGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAAt              <  1:1248093/71‑1 (MQ=255)
                                                        cgcgGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAAtt             >  1:2738719/1‑71 (MQ=255)
                                                           ggCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGcc          <  1:349926/71‑1 (MQ=255)
                                                                ggCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGAcc     <  1:3009003/71‑1 (MQ=255)
                                                                 gCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcgcg                 <  1:3701955/58‑1 (MQ=255)
                                                                 gCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCa    >  1:1445113/1‑71 (MQ=255)
                                                                 gCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCa    <  1:3257223/71‑1 (MQ=255)
                                                                  cGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCTAATTGCCGGACCAt   <  1:2088455/71‑1 (MQ=255)
                                                                   gATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATc  >  1:4579692/1‑71 (MQ=255)
                                                                   |                                                                      
GTGGCCGCGCCACGTCACTGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATC  >  Exported/837020‑837157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: