| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 837,106 | C→G | 15.1% | Q97E (CAG→GAG) | narJ → | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 837,106 | 0 | C | G | 15.1% | 125.1 / 14.2 | 53 | Q97E (CAG→GAG) | narJ | molybdenum‑cofactor‑assembly chaperone subunit (delta subunit) of nitrate reductase 1 |
| Reads supporting (aligned to +/- strand): ref base C (21/24); new base G (3/5); total (24/29) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.15e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
TGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAG > Exported/837038‑837174 | tGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAg > 1:2359348/1‑71 (MQ=255) gctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAg > 1:2021444/3‑70 (MQ=255) ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGt < 1:4773093/69‑1 (MQ=255) ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTa < 1:1249391/70‑1 (MQ=255) ctGCTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTa > 1:2197833/2‑71 (MQ=255) cTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACg < 1:241880/70‑1 (MQ=255) cTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGa < 1:1022181/71‑1 (MQ=255) cTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGa < 1:229261/71‑1 (MQ=255) cTGTTCGAACATGTTCATGCCGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGa < 1:2408335/71‑1 (MQ=255) gTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGag > 1:386382/1‑70 (MQ=255) gTTTGAACATGTGCACGGCGAATCCCGCGAC‑‑CGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagcag > 1:2686838/1‑71 (MQ=255) tttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGag < 1:2469044/69‑1 (MQ=255) ttGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagca < 1:600224/70‑1 (MQ=255) tGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGagcag < 1:2064394/70‑1 (MQ=255) gAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGa < 1:2838369/65‑1 (MQ=255) aTGTGCACGGCGAATCCCGCGACC‑CGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAgcagca < 1:4636300/66‑1 (MQ=255) tgCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTg < 1:2243148/71‑1 (MQ=255) cACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGc < 1:2103049/70‑1 (MQ=255) ggCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGt > 1:5424827/1‑70 (MQ=255) ggCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGt > 1:3496941/1‑70 (MQ=255) ggCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACATGCTGGCGCAGTACGAGCAGCACGGCTTGCAGt > 1:5243615/1‑70 (MQ=255) gCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTa < 1:4200296/71‑1 (MQ=255) ccGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGAAAAGGTCGGCCTGCAgct < 1:4813993/69‑3 (MQ=255) cGAGTCCCGCGATCGCGGCCAGGCAATGGTGGACCTGCTGGCGGAGTATGAAAAGGTCGGCCTGCAgctg > 1:1174080/1‑67 (MQ=255) cccGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGc > 1:5614802/1‑71 (MQ=255) cgcgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcg < 1:219901/71‑1 (MQ=255) cgcgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcg > 1:1229473/1‑71 (MQ=255) gcgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcgc > 1:4320596/1‑71 (MQ=255) gcgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcg > 1:2388511/1‑70 (MQ=255) cgACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcgcg < 1:1779828/71‑1 (MQ=255) gACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGa < 1:2819522/71‑1 (MQ=255) gACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGa < 1:284705/71‑1 (MQ=255) ccGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAAt < 1:1248093/71‑1 (MQ=255) cgcgGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAAtt > 1:2738719/1‑71 (MQ=255) ggCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGcc < 1:349926/71‑1 (MQ=255) ggCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGAcc < 1:3009003/71‑1 (MQ=255) gCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCcgcg < 1:3701955/58‑1 (MQ=255) gCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCa > 1:1445113/1‑71 (MQ=255) gCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCa < 1:3257223/71‑1 (MQ=255) cGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCTAATTGCCGGACCAt < 1:2088455/71‑1 (MQ=255) gATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATc > 1:4579692/1‑71 (MQ=255) tggtggACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTaaa > 1:3874702/1‑46 (MQ=255) gACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACa > 1:942909/1‑43 (MQ=255) gACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACa > 1:2935956/1‑43 (MQ=255) ctgctgGCGCAGTACGAGCAGCACGGCTTGCAGTTaa < 1:3325432/37‑1 (MQ=255) ctgctgGCGCAGTACGAGCAGCACGGCTTGCAGTTaa < 1:1507171/37‑1 (MQ=255) ctgctgGCGCAGTACGAGCAGCACGGCTTGCAGTTAAAc < 1:4240336/39‑1 (MQ=255) ctgctgGCGCAGTACGAGCAGCACGGCTTGCAGTTAAAc < 1:3137339/39‑1 (MQ=255) gctgGCGCAGTACGAGCAGCACGGCTTGCAGTTAAAc > 1:1851493/1‑37 (MQ=255) ctgGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTg > 1:4333393/1‑60 (MQ=255) ctgGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTg > 1:3206413/1‑60 (MQ=255) ctgGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTg > 1:127286/1‑60 (MQ=255) ggCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTg > 1:225439/1‑70 (MQ=255) gcgcAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTgg < 1:4604577/70‑1 (MQ=255) gcgcAGTACGAGCAGCACAGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTg < 1:442613/69‑1 (MQ=255) cgcAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAg > 1:4870630/1‑71 (MQ=255) cgcAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAg > 1:5282529/1‑71 (MQ=255) cgcAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAg > 1:821237/1‑71 (MQ=255) | TGTTGCTGTTTGAACATGTGCACGGCGAATCCCGCGACCGCGGCCAGGCGATGGTGGACCTGCTGGCGCAGTACGAGCAGCACGGCTTGCAGTTAAACAGCCGCGAATTGCCGGACCATCTGCCGCTGTATCTGGAG > Exported/837038‑837174 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |