Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 837,722 T→G 23.6% G65G (GGT→GGG narI → nitrate reductase 1, gamma (cytochrome b(NR)) subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported837,7220TG23.6% 118.8 / 45.7 76G65G (GGT→GGGnarInitrate reductase 1, gamma (cytochrome b(NR)) subunit
Reads supporting (aligned to +/- strand):  ref base T (29/29);  new base G (3/15);  total (32/44)
Fisher's exact test for biased strand distribution p-value = 1.45e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGAAAA  >  Exported/837653‑837792
                                                                     |                                                                      
gCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGt                                                                        >  1:3104546/1‑70 (MQ=255)
    gATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCActt                                                                   <  1:537561/71‑1 (MQ=255)
    gATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCAc                                                                     <  1:753902/69‑1 (MQ=255)
      tCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCActtct                                                                 <  1:2740018/71‑1 (MQ=255)
      tCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCActtct                                                                 >  1:5613210/1‑71 (MQ=255)
        gCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCa                                                                      <  1:3957735/64‑1 (MQ=255)
        gCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCActtctt                                                                <  1:4692638/70‑1 (MQ=255)
          aaaGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCgg                                                             <  1:1799919/71‑1 (MQ=255)
            aGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa                                                           >  1:633968/1‑71 (MQ=255)
             gggATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCg                                                              >  1:4080490/1‑67 (MQ=255)
             gggATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCg                                                              >  1:1547980/1‑67 (MQ=255)
               gATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa                                                           >  1:1127883/1‑68 (MQ=255)
                  gAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGa                                                     >  1:4804580/1‑71 (MQ=255)
                     ccTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACttcttc                                                               <  1:5253222/58‑1 (MQ=255)
                      cTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGAcgcc                                                 <  1:1498757/71‑1 (MQ=255)
                       tGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGAcgccg                                                >  1:1827984/1‑71 (MQ=255)
                            tCGAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCAc                                                                     <  1:5684795/45‑1 (MQ=16)
                            tCGAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCAc                                                                     <  1:2456482/45‑1 (MQ=16)
                            tCGAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCAc                                                                     <  1:1052377/45‑1 (MQ=16)
                            tCGAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTg                                           <  1:5667574/71‑1 (MQ=255)
                              gAATCTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGa                                         >  1:1772263/1‑71 (MQ=255)
                               aatcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGAt                                        >  1:4149972/4‑71 (MQ=255)
                                atcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACt                                            <  1:4370513/64‑1 (MQ=255)
                                atcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGATg                                       <  1:3530681/69‑1 (MQ=255)
                                atcTGTTCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACGCCGCACTGGATg                                       <  1:4836129/69‑1 (MQ=255)
                                aCCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa                                                           <  1:4547925/51‑1 (MQ=255)
                                aCCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTa                                                           <  1:3078725/51‑1 (MQ=255)
                                   tGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTAt                                    >  1:3413289/1‑71 (MQ=255)
                                   tGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTAt                                    >  1:3813579/1‑71 (MQ=255)
                                    gTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGt                                      <  1:1693949/68‑1 (MQ=255)
                                     ttCCATATCGGGATTCTGGGGATTTTTGTCGGTCActtctt                                                                >  1:2709699/1‑41 (MQ=255)
                                     ttCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCgg                                                             <  1:3601887/44‑1 (MQ=255)
                                     ttCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCgg                                                             <  1:2088660/44‑1 (MQ=255)
                                     ttCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCAt                                                          <  1:4474413/47‑1 (MQ=16)
                                     ttCCATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCAt                                                          <  1:1660383/47‑1 (MQ=16)
                                       ccATATCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgt                                                        >  1:453948/1‑46 (MQ=255)
                                         atatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATGTTAACCCCGCACTGGATg                                       <  1:1901880/62‑1 (MQ=255)
                                          tatCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt                                                       <  1:625380/45‑1 (MQ=255)
                                          tatCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt                                                       <  1:1612111/45‑1 (MQ=255)
                                          tatCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGcc                              <  1:2106917/70‑1 (MQ=255)
                                          tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCgg                                                             <  1:2237207/39‑1 (MQ=16)
                                          tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgtt                                                       <  1:4838486/45‑3 (MQ=255)
                                          tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgtt                                                       <  1:2480542/45‑3 (MQ=255)
                                          tatCGGCATTTTGGGGATTTTCTTCGGGCACTTGTTCGGCATgtt                                                       <  1:1282650/45‑3 (MQ=255)
                                             cGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGc                          >  1:4708655/1‑71 (MQ=255)
                                                 aTTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGt                                      <  1:3749098/55‑1 (MQ=255)
                                                 aTTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGcc                      <  1:3701736/71‑1 (MQ=255)
                                                  ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt                                                       <  1:5320668/37‑1 (MQ=255)
                                                  ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt                                                       <  1:208765/37‑1 (MQ=255)
                                                  ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCt                                                       <  1:3504586/37‑1 (MQ=255)
                                                  ttCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGcc                      <  1:4949025/70‑1 (MQ=255)
                                                    cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg                                           >  1:2619851/1‑47 (MQ=255)
                                                    cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGAt                   >  1:974300/1‑71 (MQ=255)
                                                    cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGAt                   >  1:365107/1‑71 (MQ=255)
                                                    cTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGACGCACTGGATGTATGAAGCCTGGCTGCCGAt                   >  1:5258217/1‑71 (MQ=255)
                                                       gggATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTg                 <  1:878421/70‑1 (MQ=255)
                                                       gggATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGa                <  1:3201461/71‑1 (MQ=255)
                                                        ggATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTAt                                    <  1:15432/50‑1 (MQ=255)
                                                         gATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAg              >  1:3198810/1‑71 (MQ=255)
                                                          aTTTTTGTCGGTCACTTCTTCGGTATGCTGAcgcc                                                 >  1:3579565/1‑35 (MQ=255)
                                                          aTTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg                                           >  1:4697662/1‑41 (MQ=255)
                                                          aTTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg                                           >  1:3820107/1‑41 (MQ=255)
                                                          aTTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGt             <  1:488473/71‑1 (MQ=255)
                                                            ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg                                           <  1:4550046/39‑1 (MQ=255)
                                                            ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg                                           <  1:2713587/39‑1 (MQ=255)
                                                            ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTg                                           <  1:4893634/39‑1 (MQ=255)
                                                            ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAg              >  1:174615/1‑68 (MQ=255)
                                                            ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGt             >  1:3932177/1‑69 (MQ=255)
                                                            ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTg            <  1:3362383/70‑1 (MQ=255)
                                                            ttttGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTg            >  1:24118/1‑70 (MQ=255)
                                                                  cGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGa                    >  1:5669370/1‑56 (MQ=255)
                                                                  cGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGa                    >  1:3958079/1‑56 (MQ=255)
                                                                  cGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGa                    >  1:1508084/1‑56 (MQ=255)
                                                                   ggTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTg                            >  1:3190265/1‑47 (MQ=255)
                                                                   ggTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGa     >  1:3720324/1‑70 (MQ=255)
                                                                   ggTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGa     >  1:5684658/1‑70 (MQ=255)
                                                                   ggTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGa     >  1:2060130/1‑70 (MQ=255)
                                                                     tCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACATaaaa  <  1:2201898/71‑1 (MQ=255)
                                                                     |                                                                      
GCTGGATCGCAAAGGGATGAACCTGGCGTCGAACCTGTTCCATATCGGGATTCTGGGGATTTTTGTCGGTCACTTCTTCGGTATGCTGACGCCGCACTGGATGTATGAAGCCTGGCTGCCGATTGAAGTGAAACAGAAAA  >  Exported/837653‑837792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: