Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 199,598 A→C 6.7% V52V (GTT→GTG metQ ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported199,5980AC 6.7% 235.7 / 9.4 90V52V (GTT→GTGmetQDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (53/31);  new base C (4/2);  total (57/33)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

TGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTG  >  Exported/199529‑199665
                                                                     |                                                                   
tGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCaa                                                                    >  1:3594144/1‑71 (MQ=255)
tGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCaa                                                                    >  1:84407/1‑71 (MQ=255)
 gCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAAc                                                                   >  1:4811834/1‑71 (MQ=255)
     cGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACtt                                                                 <  1:2010151/69‑1 (MQ=255)
      gTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATATGTCTTTCGCa                                                                     >  1:1984489/1‑64 (MQ=255)
         tGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcg                                                           >  1:4789289/1‑71 (MQ=255)
         tGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcg                                                           >  1:2401569/1‑71 (MQ=255)
           gCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcgc                                                          <  1:3550483/70‑1 (MQ=255)
             aGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCTCAACTTTCTGCGCGAc                                                       <  1:2215594/71‑1 (MQ=255)
                 cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACt                                                                  >  1:4177215/1‑56 (MQ=255)
                 cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACt                                                                  >  1:5668060/1‑56 (MQ=255)
                 cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACt                                                                  >  1:5157729/1‑56 (MQ=255)
                 cATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCt                                                   >  1:4673807/1‑71 (MQ=255)
                  aTAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCt                                                   <  1:1337058/70‑1 (MQ=255)
                    aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACtt                                                                 >  1:2920160/1‑54 (MQ=255)
                    aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACtt                                                                 >  1:358721/1‑54 (MQ=255)
                    aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCt                                                              >  1:4783052/1‑57 (MQ=255)
                    aGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCt                                                              >  1:2191452/1‑57 (MQ=255)
                     gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa                                                <  1:3098313/70‑1 (MQ=255)
                     gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa                                                <  1:4693274/70‑1 (MQ=255)
                     gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa                                                <  1:2439409/70‑1 (MQ=255)
                        gTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTc                                                               >  1:1118941/1‑52 (MQ=255)
                        gTTGAAGGTTACCAGCTCAACGTCCAGGCCATATATGTCTTTCGCAACTTTc                                                               >  1:135150/1‑52 (MQ=255)
                         ttGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCaa                                               <  1:3199998/67‑1 (MQ=255)
                           gAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACttt                                                                >  1:4082564/1‑48 (MQ=255)
                            aaGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                        >  1:498431/1‑71 (MQ=255)
                            aaGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                        >  1:3125641/1‑71 (MQ=255)
                               gTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc                                     <  1:237018/71‑1 (MQ=255)
                               cctACCAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTc                                     >  1:44007/3‑71 (MQ=255)
                                ttACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgc                                                            <  1:1820407/47‑1 (MQ=255)
                                ttACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgc                                                            <  1:444054/47‑1 (MQ=255)
                                  aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcgc                                                          >  1:3396982/1‑47 (MQ=255)
                                  aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTgcgc                                                          >  1:2015192/1‑47 (MQ=255)
                                  aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctg                                           >  1:1912610/1‑62 (MQ=255)
                                   ccAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcg                                 >  1:4296210/1‑70 (MQ=255)
                                    cAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcgc                                <  1:4101298/71‑3 (MQ=255)
                                    cAGCTCAACGTCCAGGCCATATATCACTTTCGCAACATTCTGCCCGACTTCTGCAACCTGCTGtt                                      >  1:4735878/1‑65 (MQ=255)
                                     agttCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGcc                               <  1:1546961/68‑1 (MQ=255)
                                         cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACt                                                      >  1:4723275/1‑44 (MQ=255)
                                         cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac                            >  1:3109691/1‑70 (MQ=255)
                                         cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac                            >  1:4920648/1‑70 (MQ=255)
                                         cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat                             >  1:2335649/1‑68 (MQ=255)
                                         cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat                             >  1:661592/1‑68 (MQ=255)
                                          aaCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac                            <  1:553301/69‑1 (MQ=255)
                                           aCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTACTGCAACCTGCTGTTCGGCACCAACAAt                         <  1:2688514/71‑1 (MQ=255)
                                              tCCAGGCCATATTTGTCTTTCGCAACTTTCTTCGCGAc                                                       <  1:2473881/38‑1 (MQ=255)
                                              tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGAc                                                       <  1:989113/38‑1 (MQ=255)
                                              tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGAc                                                       <  1:3847435/38‑1 (MQ=255)
                                              tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc                      <  1:3783933/71‑1 (MQ=255)
                                              tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc                      >  1:3192328/1‑71 (MQ=255)
                                                cAGGCCTTATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGc                    >  1:732471/1‑71 (MQ=255)
                                                cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc                                     <  1:5571799/54‑1 (MQ=255)
                                                cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc                                     <  1:2996453/54‑1 (MQ=255)
                                                 aGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGcc                   <  1:946684/71‑1 (MQ=255)
                                                  ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                              >  1:624659/1‑43 (MQ=255)
                                                  ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                              >  1:4501396/1‑43 (MQ=255)
                                                  ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCa                       >  1:2362217/1‑66 (MQ=255)
                                                    ccATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACg                     >  1:4402586/1‑66 (MQ=255)
                                                     cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCgg                                   >  1:2293087/1‑51 (MQ=255)
                                                     cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCgg                                   >  1:2581423/1‑51 (MQ=255)
                                                     cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACt               >  1:2912895/1‑71 (MQ=255)
                                                     cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACt               >  1:4797009/1‑71 (MQ=255)
                                                      atatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGAc                >  1:1435920/1‑69 (MQ=255)
                                                      atatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCAGCACCAACAATCACGCCGACtt              <  1:5226942/71‑1 (MQ=255)
                                                       tatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACttt             <  1:4599779/71‑1 (MQ=255)
                                                        atTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACttt             <  1:4870568/70‑1 (MQ=255)
                                                         tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                              >  1:5120446/1‑36 (MQ=255)
                                                         tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                              >  1:548530/1‑36 (MQ=255)
                                                         tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                              >  1:2795265/1‑36 (MQ=255)
                                                         tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGc                                  >  1:3245063/1‑48 (MQ=255)
                                                         tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCa                                 >  1:3503704/1‑49 (MQ=255)
                                                         tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCACCAATc                        >  1:1993699/1‑58 (MQ=255)
                                                         tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTaa           >  1:4701123/1‑71 (MQ=255)
                                                         tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTa            <  1:4414412/70‑1 (MQ=255)
                                                          ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                              >  1:1296223/1‑35 (MQ=255)
                                                          ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                        <  1:5334055/41‑1 (MQ=255)
                                                          ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                        <  1:2149686/41‑1 (MQ=255)
                                                          ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                        <  1:904095/41‑1 (MQ=255)
                                                          ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                        <  1:4268615/41‑1 (MQ=255)
                                                          ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt          >  1:5197455/1‑71 (MQ=255)
                                                          ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt          >  1:4436240/1‑71 (MQ=255)
                                                          ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt          >  1:2851138/1‑71 (MQ=255)
                                                          ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt          >  1:3959560/1‑71 (MQ=255)
                                                           tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtg         >  1:3724639/1‑71 (MQ=255)
                                                           tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtg         >  1:3717946/1‑71 (MQ=255)
                                                             tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGa                 >  1:3354070/1‑61 (MQ=255)
                                                             tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtgtg       <  1:2101692/71‑1 (MQ=255)
                                                              cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTgg      >  1:2120852/1‑71 (MQ=255)
                                                               tttCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATc                        >  1:291920/1‑52 (MQ=255)
                                                                 tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTgg      >  1:5155188/1‑68 (MQ=255)
                                                                 tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGtt    <  1:345309/70‑1 (MQ=255)
                                                                  cGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTg  >  1:121920/1‑71 (MQ=255)
                                                                    cAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac                            <  1:1999374/43‑1 (MQ=255)
                                                                     |                                                                   
TGCTTCGTTTGGCAGAACATAGTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTG  >  Exported/199529‑199665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: