| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 980,296 | G→A | 7.5% | N3N (AAC→AAT) | cspI ← | cold shock protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 980,296 | 0 | G | A | 7.5% | 311.8 / 10.0 | 106 | N3N (AAC→AAT) | cspI | cold shock protein |
| Reads supporting (aligned to +/- strand): ref base G (53/45); new base A (3/5); total (56/50) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.50e-01 | |||||||||||
Only 100 of 108 total aligned reads displayed.
CCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCA > Exported/980228‑980362 | ccATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4351269/70‑1 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa > 1:5261761/1‑67 (MQ=255) tttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa > 1:1425652/1‑71 (MQ=255) ttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa > 1:4028751/1‑70 (MQ=255) ttCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACa > 1:2488971/1‑70 (MQ=255) tCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACAt > 1:2333624/1‑70 (MQ=255) cGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATaa > 1:151545/1‑71 (MQ=255) gTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:2103233/1‑70 (MQ=255) tgatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCTCTAAACCAGTCATTTTGTTAGACATAatta < 1:4387484/69‑1 (MQ=255) gatgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatta < 1:3978299/68‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4986770/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:5633836/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:210327/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:4753505/55‑1 (MQ=255) atgaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt < 1:8492/55‑1 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg > 1:4645415/1‑52 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTg > 1:2865127/1‑52 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt < 1:4098546/64‑1 (MQ=255) gaAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAatt < 1:1845520/64‑1 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAATCCAGTCATTTTGTTAGACATAATTATTAc > 1:3841039/1‑68 (MQ=255) aaaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCtt > 1:923052/1‑71 (MQ=255) aaCCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCttt > 1:4390881/1‑71 (MQ=255) ccAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg < 1:2256864/71‑1 (MQ=255) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:5017903/1‑70 (MQ=255) cAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:2594071/1‑70 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGt > 1:5375720/1‑47 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:2074788/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:5498046/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:5448399/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3169106/1‑69 (MQ=255) aaaaCCTTTTTCAGGGTTAAACCATTTCACTAAACAAGTCATTTTGt > 1:2076230/1‑47 (MQ=255) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTa > 1:2629414/1‑48 (MQ=255) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3407416/1‑68 (MQ=255) aaaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTg > 1:3166722/1‑68 (MQ=255) aaCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaaga < 1:405913/71‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTATACCAGTCATTTTGt < 1:5152605/44‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat > 1:4194263/1‑57 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat > 1:4752905/1‑57 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAattat > 1:2418489/1‑57 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:2837519/71‑1 (MQ=255) aCCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:5581304/71‑1 (MQ=255) ccTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa > 1:691397/1‑71 (MQ=255) cTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt < 1:2764800/71‑1 (MQ=255) tttatCAGCGTTAAACCATTTTACTAAACCAGTCATTTTATTTGa < 1:1796779/45‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt < 1:2627508/69‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAtt < 1:4040715/69‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTa < 1:5659966/70‑1 (MQ=255) tttCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAg > 1:697425/1‑71 (MQ=255) tCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:2869713/70‑1 (MQ=255) tCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:1376651/70‑1 (MQ=255) tCAGCGTTAAACCATTTTACTAAACCAGTCATTTTAttt < 1:3098439/39‑2 (MQ=25) cAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:778949/69‑1 (MQ=255) aGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCt < 1:1923509/71‑1 (MQ=255) ttAAACCATTTTACTAAACCAGTCATTTTATTTGa < 1:5405127/35‑1 (MQ=25) ttAAACCATTTTACTAAACCAGTCATTTTATTAGACAt < 1:3734753/38‑1 (MQ=38) ttAAACCATTTTACTAAACCAGTCATTTTATTAGACAt < 1:26667/38‑1 (MQ=38) ttAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAat < 1:2705755/41‑1 (MQ=255) tAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAAt < 1:5585337/59‑1 (MQ=255) aaaCCATTTTACTAAACCAGTCATTTTATTAGACATat > 1:208707/1‑37 (MQ=37) aaaCCATTTTACTAAACCAGTCATTTTATTAGACATAtttta > 1:2586244/1‑40 (MQ=37) aaaCCATTTTACTAAACCAGTCATTTTATTAGACATAtttta > 1:4194764/1‑40 (MQ=37) aaCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAg < 1:822315/71‑1 (MQ=255) aaCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAg < 1:1135570/71‑1 (MQ=255) aaCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAg < 1:5210032/71‑1 (MQ=255) aCCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:1228825/1‑39 (MQ=255) aCCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:3855462/1‑39 (MQ=255) aCCATTTCACTAAACCAGTCATTTTGTTAGACATAatta > 1:3903845/1‑39 (MQ=255) aCCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTg < 1:5656010/65‑1 (MQ=255) tttCACTAAACCAGTCATTTTGTTAGACATAatta > 1:1697838/1‑35 (MQ=255) tttCACTAAACCAGTCATTTTGTTAGACATAatta > 1:1317535/1‑35 (MQ=255) tttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTgaagaa < 1:805918/50‑1 (MQ=255) tttCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATgg < 1:324009/71‑1 (MQ=255) tCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa > 1:1662990/1‑49 (MQ=255) cACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTcc > 1:2260131/1‑71 (MQ=255) cTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTg < 1:1574227/56‑1 (MQ=255) cTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGa < 1:1615037/71‑1 (MQ=255) tAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGa > 1:4667678/1‑70 (MQ=255) aaaCCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTa < 1:4349363/47‑1 (MQ=255) aaaCCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGaa > 1:1268701/1‑70 (MQ=255) aaCCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa > 1:4647215/1‑43 (MQ=255) aaCCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa > 1:5162719/1‑43 (MQ=255) aaCCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGaaa > 1:3400259/1‑43 (MQ=255) aaCCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGaaa < 1:2944775/70‑1 (MQ=255) gTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCtt < 1:2945207/47‑1 (MQ=255) gTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAat < 1:5500449/71‑1 (MQ=255) tCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGc < 1:1190865/42‑1 (MQ=255) tCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTgg < 1:3166608/48‑1 (MQ=255) tCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTgg < 1:908996/48‑1 (MQ=255) tCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATg < 1:2905249/56‑1 (MQ=255) tCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAata < 1:1167003/71‑1 (MQ=255) aTTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCt > 1:2719502/1‑43 (MQ=255) aTTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAatat > 1:1868999/1‑70 (MQ=255) aTTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAatat > 1:5624157/1‑70 (MQ=255) aTTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAatat > 1:2534053/1‑70 (MQ=255) aTTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAatat < 1:4326628/70‑1 (MQ=255) tttGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCt > 1:2000319/1‑41 (MQ=255) tttGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCt > 1:1543214/1‑41 (MQ=255) ttGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCa > 1:3122699/1‑69 (MQ=255) tGTTAGACATAATTATTACCTTTTGAAGAAATTAGccc > 1:13992/1‑38 (MQ=255) tGTTAGACATAATTATTACCTTTTGAAGAAATTAGccc > 1:4148454/1‑38 (MQ=255) | CCATCTTTCGGCGTGATGAAACCAAAACCTTTTTCAGGGTTAAACCATTTCACTAAACCAGTCATTTTGTTAGACATAATTATTACCTTTTGAAGAAATTAGCCCTTGGGCAGAATGGTCCGAAAAAAAATATCA > Exported/980228‑980362 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |