Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 199,619 A→G 8.0% V45V (GTT→GTC metQ ← DL‑methionine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported199,6190AG 8.0% 244.2 / 8.8 87V45V (GTT→GTCmetQDL‑methionine transporter subunit
Reads supporting (aligned to +/- strand):  ref base A (52/28);  new base G (5/2);  total (57/30)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.06e-01

GTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCG  >  Exported/199550‑199685
                                                                     |                                                                  
gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa                                                                    <  1:3098313/70‑1 (MQ=255)
gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa                                                                    <  1:2439409/70‑1 (MQ=255)
gTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCa                                                                    <  1:4693274/70‑1 (MQ=255)
    ttGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCaa                                                                   <  1:3199998/67‑1 (MQ=255)
       aaGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                                            >  1:3125641/1‑71 (MQ=255)
       aaGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                                            >  1:498431/1‑71 (MQ=255)
          gTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc                                                         <  1:237018/71‑1 (MQ=255)
          cctACCAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTc                                                         >  1:44007/3‑71 (MQ=255)
             aCCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctg                                                               >  1:1912610/1‑62 (MQ=255)
              ccAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcg                                                     >  1:4296210/1‑70 (MQ=255)
               cAGTTCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGcgc                                                    <  1:4101298/71‑3 (MQ=255)
               cAGCTCAACGTCCAGGCCATATATCACTTTCGCAACATTCTGCCCGACTTCTGCAACCTGCTGtt                                                          >  1:4735878/1‑65 (MQ=255)
                agttCAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGcc                                                   <  1:1546961/68‑1 (MQ=255)
                    cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac                                                >  1:4920648/1‑70 (MQ=255)
                    cAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac                                                >  1:3109691/1‑70 (MQ=255)
                    cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat                                                 >  1:661592/1‑68 (MQ=255)
                    cAACATCGAGGCCATATTTCTCTTTTGCCACCTTTTTAGCGACTTCCGCGACATCTTGTTCTGCGCcat                                                 >  1:2335649/1‑68 (MQ=255)
                     aaCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac                                                <  1:553301/69‑1 (MQ=255)
                      aCGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTACTGCAACCTGCTGTTCGGCACCAACAAt                                             <  1:2688514/71‑1 (MQ=255)
                         tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc                                          >  1:3192328/1‑71 (MQ=255)
                         tCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCAc                                          <  1:3783933/71‑1 (MQ=255)
                           cAGGCCTTATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGc                                        >  1:732471/1‑71 (MQ=255)
                           cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc                                                         <  1:2996453/54‑1 (MQ=255)
                           cAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTc                                                         <  1:5571799/54‑1 (MQ=255)
                            aGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGcc                                       <  1:946684/71‑1 (MQ=255)
                             ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                                                  >  1:4501396/1‑43 (MQ=255)
                             ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                                                  >  1:624659/1‑43 (MQ=255)
                             ggCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCa                                           >  1:2362217/1‑66 (MQ=255)
                               ccATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACg                                         >  1:4402586/1‑66 (MQ=255)
                                cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCgg                                                       >  1:2581423/1‑51 (MQ=255)
                                cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCgg                                                       >  1:2293087/1‑51 (MQ=255)
                                cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACt                                   >  1:2912895/1‑71 (MQ=255)
                                cATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACt                                   >  1:4797009/1‑71 (MQ=255)
                                 atatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGAc                                    >  1:1435920/1‑69 (MQ=255)
                                 atatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCAGCACCAACAATCACGCCGACtt                                  <  1:5226942/71‑1 (MQ=255)
                                  tatTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACttt                                 <  1:4599779/71‑1 (MQ=255)
                                   atTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACttt                                 <  1:4870568/70‑1 (MQ=255)
                                    tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                                                  >  1:2795265/1‑36 (MQ=255)
                                    tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                                                  >  1:548530/1‑36 (MQ=255)
                                    tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                                                  >  1:5120446/1‑36 (MQ=255)
                                    tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGc                                                      >  1:3245063/1‑48 (MQ=255)
                                    tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCa                                                     >  1:3503704/1‑49 (MQ=255)
                                    tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCACCAATc                                            >  1:1993699/1‑58 (MQ=255)
                                    tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTaa                               >  1:4701123/1‑71 (MQ=255)
                                    tttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTa                                <  1:4414412/70‑1 (MQ=255)
                                     ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAAc                                                                  >  1:1296223/1‑35 (MQ=255)
                                     ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                                            <  1:2149686/41‑1 (MQ=255)
                                     ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                                            <  1:5334055/41‑1 (MQ=255)
                                     ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                                            <  1:904095/41‑1 (MQ=255)
                                     ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACctgctg                                                            <  1:4268615/41‑1 (MQ=255)
                                     ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt                              >  1:4436240/1‑71 (MQ=255)
                                     ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt                              >  1:3959560/1‑71 (MQ=255)
                                     ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt                              >  1:5197455/1‑71 (MQ=255)
                                     ttGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAt                              >  1:2851138/1‑71 (MQ=255)
                                      tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtg                             >  1:3717946/1‑71 (MQ=255)
                                      tGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtg                             >  1:3724639/1‑71 (MQ=255)
                                        tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGa                                     >  1:3354070/1‑61 (MQ=255)
                                        tCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAAtgtg                           <  1:2101692/71‑1 (MQ=255)
                                         cTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTgg                          >  1:2120852/1‑71 (MQ=255)
                                          tttCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATc                                            >  1:291920/1‑52 (MQ=255)
                                            tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTgg                          >  1:5155188/1‑68 (MQ=255)
                                            tCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGtt                        <  1:345309/70‑1 (MQ=255)
                                             cGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTg                      >  1:121920/1‑71 (MQ=255)
                                               cAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACcaac                                                <  1:1999374/43‑1 (MQ=255)
                                                   tttCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtt                >  1:1769013/1‑71 (MQ=255)
                                                     tCTGCGCGCCTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtttt              >  1:5476276/1‑71 (MQ=255)
                                                     tCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACg                                         >  1:1042255/1‑44 (MQ=255)
                                                     tCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtttt              >  1:5141071/1‑71 (MQ=255)
                                                      cTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttt             >  1:5647715/1‑71 (MQ=255)
                                                      cTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCtttt              <  1:3818655/70‑1 (MQ=255)
                                                        gcgcGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTg                      <  1:1765505/60‑1 (MQ=255)
                                                         cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttttt             <  1:4991990/68‑1 (MQ=255)
                                                         cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCa           <  1:4383731/70‑1 (MQ=255)
                                                         cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCa           <  1:4940091/70‑1 (MQ=255)
                                                         cgcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCa           <  1:5305873/70‑1 (MQ=255)
                                                          gcgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCg                                      >  1:3226176/1‑42 (MQ=255)
                                                           cgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATcc        >  1:476340/1‑71 (MQ=255)
                                                           cgACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATcc        >  1:2961040/1‑71 (MQ=255)
                                                            gACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCt       <  1:2163639/71‑1 (MQ=255)
                                                            gACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCt       >  1:2461757/1‑71 (MQ=255)
                                                            gACTTCCGCGACATCTTGTTCTGCGCCATTTATAACGCCAACTTTAATGTGTTTTGCATcgct               >  1:2968153/1‑60 (MQ=255)
                                                                tCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGAcc   >  1:2699028/1‑71 (MQ=255)
                                                                tCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGAc    >  1:2299905/1‑70 (MQ=255)
                                                                 cTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg  >  1:2364959/1‑71 (MQ=255)
                                                                 cTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg  >  1:5150116/1‑71 (MQ=255)
                                                                  tGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg  >  1:3483311/1‑70 (MQ=255)
                                                                  tGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg  >  1:450319/1‑70 (MQ=255)
                                                                  tGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCg  >  1:697501/1‑70 (MQ=255)
                                                                   gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt               <  1:62238/56‑1 (MQ=255)
                                                                   gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt               <  1:2766342/56‑1 (MQ=255)
                                                                   gCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCttt               <  1:1587460/56‑1 (MQ=255)
                                                                     |                                                                  
GTCGTTGAAGGTTACCAGCTCAACGTCCAGGCCATATTTGTCTTTCGCAACTTTCTGCGCGACTTCTGCAACCTGCTGTTCGGCACCAACAATCACGCCGACTTTAATGTGGTTTGGATCTTTTTCATCCTGACCG  >  Exported/199550‑199685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: