Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 983,531 A→C 13.7% intergenic (+443/‑125) ydfC → / → dicB hypothetical protein/cell division inhibition protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported983,5310AC13.7% 109.3 / 14.2 51intergenic (+443/‑125)ydfC/dicBhypothetical protein/cell division inhibition protein
Reads supporting (aligned to +/- strand):  ref base A (19/25);  new base C (2/5);  total (21/30)
Fisher's exact test for biased strand distribution p-value = 6.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.45e-01

CGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGTTAACG  >  Exported/983463‑983601
                                                                    |                                                                      
cGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa                                                                       >  1:3093459/1‑70 (MQ=255)
  tGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAg                                                                    >  1:3683709/1‑71 (MQ=255)
    aCTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGt                                                                   >  1:3041768/1‑70 (MQ=255)
        ctctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTaaaa                                                              >  1:44310/1‑71 (MQ=255)
          ctGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAAc                                                             >  1:3472261/1‑70 (MQ=255)
             ccgccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaa                                                         <  1:701072/71‑1 (MQ=255)
               gccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                       >  1:3810154/1‑71 (MQ=255)
               gccCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                       >  1:2859085/1‑71 (MQ=255)
                cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                       >  1:3801619/1‑70 (MQ=255)
                cccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaa                                                          <  1:2318810/67‑1 (MQ=255)
                 ccTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAg                                                                    >  1:2177498/1‑56 (MQ=255)
                      ttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaa                                                        <  1:1153227/63‑1 (MQ=255)
                      ttAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAgtgt                                                 <  1:3432727/70‑1 (MQ=255)
                      ttAAAGAGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAgtgt                                                 >  1:60501/1‑70 (MQ=255)
                       tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa                                                                       >  1:3522728/1‑47 (MQ=255)
                       tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa                                                                       >  1:4790084/1‑47 (MQ=255)
                       tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGaa                                                                       >  1:2777883/1‑47 (MQ=255)
                       tAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt                                                <  1:4752168/70‑1 (MQ=255)
                        aaaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc                                                      >  1:5115125/1‑63 (MQ=255)
                        aaaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc                                                      >  1:4377844/1‑63 (MQ=255)
                         aaGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTAt                                              <  1:1607623/70‑1 (MQ=255)
                             gAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa                                                              >  1:1109611/1‑50 (MQ=255)
                             gAATTTTGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaaaa                                                              >  1:4340373/1‑50 (MQ=255)
                              aaTTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTg                                         >  1:1791464/1‑70 (MQ=255)
                                ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                                <  1:1661474/45‑1 (MQ=25)
                                ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                                <  1:3726905/45‑1 (MQ=25)
                                ttttGTGATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                                <  1:5535740/45‑1 (MQ=25)
                                  ttGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGAtt                                     >  1:5099483/1‑70 (MQ=255)
                                    gtgATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATtct                                   <  1:3279245/70‑1 (MQ=255)
                                      gATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                                <  1:902769/39‑1 (MQ=25)
                                      gATGCGGTGAATGCGGCTAAGCGCACGTGGCACAGTTaa                                                                <  1:4210935/39‑1 (MQ=25)
                                          tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt                                                <  1:1989506/51‑1 (MQ=255)
                                          tggtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGtt                                                <  1:4610845/51‑1 (MQ=255)
                                            gtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCg                          >  1:4111513/1‑71 (MQ=255)
                                            gtgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCg                          <  1:4843624/71‑1 (MQ=255)
                                             tgAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCgg                         <  1:1709916/71‑1 (MQ=255)
                                              gAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGc                        >  1:2829307/1‑71 (MQ=255)
                                               aaTGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                       <  1:5371144/39‑1 (MQ=255)
                                               aaTGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                       <  1:2236473/39‑1 (MQ=255)
                                               aaTGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaaaa                                                       <  1:454490/39‑1 (MQ=255)
                                               aaTGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGc                        <  1:2943790/70‑1 (MQ=255)
                                                 tGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaa                                                         <  1:3934297/35‑1 (MQ=255)
                                                 tGCGGCTGAGCGCACGCGGAACAGTTAAAACCaaa                                                         <  1:5357137/35‑1 (MQ=255)
                                                 tGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAAc                                                      >  1:4410330/1‑38 (MQ=255)
                                                 tGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGa                                       >  1:2614304/1‑53 (MQ=255)
                                                 tGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGa                                       >  1:4336838/1‑53 (MQ=255)
                                                 tGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGa                                       >  1:4063963/1‑53 (MQ=255)
                                                         aGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGtt              <  1:5331030/70‑1 (MQ=255)
                                                             cACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTg         <  1:5567380/71‑1 (MQ=255)
                                                              aCGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATcc                           <  1:161028/52‑1 (MQ=255)
                                                              aCGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTgg        <  1:4898959/71‑1 (MQ=255)
                                                                gcgGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGtt      <  1:3903837/71‑1 (MQ=255)
                                                                 cgGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGTTa     <  1:1441893/71‑1 (MQ=255)
                                                                  ggAACAGTTAAAACCAAAAACAGTTTTATTGGTGGATTCTCTGTATCCGTCTTTAATTGtt              <  1:5337640/61‑1 (MQ=255)
                                                                  ggAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGtt      <  1:1633088/69‑1 (MQ=255)
                                                                    aaCAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAAt                  <  1:1026639/55‑1 (MQ=255)
                                                                    aaCAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAAt                  <  1:5418806/55‑1 (MQ=255)
                                                                    aaCAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGTTAACg  >  1:5386824/1‑71 (MQ=255)
                                                                    aaCAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGTTAACg  >  1:2288182/1‑71 (MQ=255)
                                                                    aaCAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGTTAACg  >  1:3983770/1‑71 (MQ=255)
                                                                    |                                                                      
CGTGACTGCTCTGCCGCCCTTTTTAAAGTGAATTTTGTGATGTGGTGAATGCGGCTGAGCGCACGCGGAACAGTTAAAACCAAAAACAGTGTTATGGGTGGATTCTCTGTATCCGGCGTTAATTGTTAACTGGTTAACG  >  Exported/983463‑983601

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: