| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,602 | G→C | 18.4% | A489A (GCC→GCG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,602 | 0 | G | C | 18.4% | 141.1 / 25.0 | 65 | A489A (GCC→GCG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base G (27/26); new base C (5/7); total (32/33) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.51e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01 | |||||||||||
GCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCT > Exported/1014534‑1014666 | gCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGc > 1:838174/1‑71 (MQ=255)gCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGc > 1:2413168/1‑71 (MQ=255)gCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGc < 1:3358408/71‑1 (MQ=255) cTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGcc < 1:1576348/71‑1 (MQ=255) cTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGcc > 1:836335/1‑71 (MQ=255) cTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGcc < 1:1383428/71‑1 (MQ=255) ccATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGa > 1:3342986/1‑71 (MQ=255) cccAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGaccacc > 1:5442996/1‑71 (MQ=255) cccAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGaccacc > 1:1637925/1‑71 (MQ=255) ccAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGaccacc < 1:1525995/70‑1 (MQ=255) cAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCg > 1:1622604/1‑62 (MQ=255) gTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGcc < 1:5281274/59‑1 (MQ=255) ttCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATAc < 1:607906/71‑1 (MQ=255) cGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCg > 1:5482691/1‑56 (MQ=255) cGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCg > 1:4276901/1‑56 (MQ=255) cGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCg > 1:2233633/1‑56 (MQ=255) cGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCg > 1:1588932/1‑56 (MQ=255) ggATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGcc < 1:3217333/71‑1 (MQ=255) ttCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCgg > 1:4067478/1‑51 (MQ=255) ctcacaTTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAg > 1:1360593/3‑70 (MQ=255) aaCACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAg < 1:2520645/70‑1 (MQ=255) aaCACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAg < 1:2641148/70‑1 (MQ=255) aaCACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAg < 1:1980413/70‑1 (MQ=255) aaCACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAg < 1:4134195/70‑1 (MQ=255) acacATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGac < 1:65243/49‑1 (MQ=255) tCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCaa > 1:5568517/1‑58 (MQ=255) tCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAgccgcc > 1:924183/1‑71 (MQ=255) aGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCgtg < 1:3329862/71‑1 (MQ=255) aGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCgt < 1:2919898/70‑1 (MQ=255) gCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAgccgcc < 1:1879519/66‑1 (MQ=255) tctTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGa < 1:2680070/42‑1 (MQ=255) ctTAATACTTCCCTGCGCCAATACAGCGGCCGGac < 1:4384918/35‑1 (MQ=255) ctTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGttttt > 1:4765620/1‑71 (MQ=255) ttAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCa < 1:462459/48‑1 (MQ=255) aaaTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATa > 1:4431457/2‑41 (MQ=255) aaTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGAt > 1:5015040/1‑39 (MQ=255) aaTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATa > 1:1266360/1‑40 (MQ=255) aaTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATa > 1:4257964/1‑40 (MQ=255) aaTACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGa > 1:1823041/1‑53 (MQ=255) tACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAgg < 1:3616730/47‑1 (MQ=255) ttCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAg < 1:162245/47‑1 (MQ=255) cccTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCa < 1:5435483/69‑1 (MQ=255) cccTGCGCCAATACAGCGGCCGGACCACCGATACAGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCa < 1:3040774/69‑1 (MQ=255) cTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGcgt < 1:2813926/68‑3 (MQ=255) cTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTcgg > 1:5648506/1‑69 (MQ=255) cTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTcg > 1:5141058/1‑69 (MQ=255) cTGCGCCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTc < 1:227697/69‑1 (MQ=255) cTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCa > 1:5498553/1‑67 (MQ=255) cTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCg < 1:406601/71‑1 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGtaa > 1:4676832/1‑69 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGta < 1:2331422/70‑2 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGta < 1:2271464/70‑2 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGt < 1:4717182/69‑1 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGt < 1:2188830/69‑1 (MQ=255) cgcCAGTACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGt < 1:3096114/69‑1 (MQ=255) cgcCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTc < 1:1776041/70‑1 (MQ=255) cAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACct > 1:2937934/1‑71 (MQ=255) cAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACct > 1:5625313/1‑71 (MQ=255) agtACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACct > 1:3344664/3‑70 (MQ=255) gtACCGCCGCCGGACCGCCGATGCTACCGAGGTAGAAGCCGCCGTGTTTATGACACGCGTCGGTAACctgc > 1:401206/2‑71 (MQ=255) aTACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGccgccg > 1:3263390/1‑44 (MQ=255) aTACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCAt < 1:5582778/60‑1 (MQ=255) tACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATcc < 1:2153796/61‑1 (MQ=255) tACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACctgct > 1:477448/1‑71 (MQ=255) aCAGCGGCCGGACCACCGATACTGACAAGGTAGAAGCCGCCGTGTTTTTTAc > 1:2034702/1‑52 (MQ=255) ggCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTAc < 1:3324028/47‑1 (MQ=255) | GCTTCCATTCCCAGTTCCGGATATTCAACACATTCCAGGCTCTTAATACTTCCCTGCGCCAATACAGCGGCCGGACCACCGATACTGCCAAGGTAGAAGCCGCCGTGTTTTTTACAGGCATCCGTCACCTGCT > Exported/1014534‑1014666 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |