Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,014,731 A→G 23.0% S446S (TCT→TCC fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,014,7310AG23.0% 156.1 / 44.5 87S446S (TCT→TCCfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base A (40/27);  new base G (12/8);  total (52/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGA  >  Exported/1014664‑1014800
                                                                   |                                                                     
gctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGaa                                                                    >  1:3249287/1‑71 (MQ=255)
gctgactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGag                                                                    >  1:472056/1‑70 (MQ=255)
 ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGa                                                                     <  1:3328560/69‑1 (MQ=255)
 ctggctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCAACATAAGa                                                                     <  1:2458362/69‑1 (MQ=255)
   gactgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtc                                                                  >  1:2740101/3‑70 (MQ=255)
     ctgCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccat                                                               >  1:919753/1‑71 (MQ=255)
     ctgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtccat                                                               <  1:635382/71‑1 (MQ=255)
      tgCGGTTACCTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtccata                                                              >  1:33357/1‑70 (MQ=255)
       gCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc                                                             >  1:4892227/1‑71 (MQ=255)
          gTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatcca                                                                <  1:3370554/65‑1 (MQ=255)
           ttGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtcc                                                         >  1:2943055/1‑71 (MQ=255)
            taccTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcct                                                        >  1:2604965/3‑70 (MQ=255)
            tGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtccg                                                        >  1:2437893/1‑71 (MQ=255)
             gCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgtccg                                                        >  1:153873/1‑70 (MQ=255)
             gCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccgt                                                           <  1:4345819/67‑1 (MQ=255)
             accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg                                                       >  1:3270859/2‑69 (MQ=255)
             accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg                                                       >  1:5384115/2‑69 (MQ=255)
             accTTTCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcctg                                                       >  1:3405713/2‑69 (MQ=255)
             accTTTCGCCAGCATGATCATGCCGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCAtacggcct                                                        >  1:5142767/2‑69 (MQ=255)
               cTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCcggcg                                                     >  1:2273606/1‑71 (MQ=255)
                tttCGCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAgtcca                                                                <  1:4624087/59‑1 (MQ=255)
                 ttCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt                                                   >  1:3373890/1‑71 (MQ=255)
                  tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGt                                                   <  1:1482870/70‑1 (MQ=255)
                  tCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTc                                                  >  1:4617686/1‑71 (MQ=255)
                    gCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCggtggt                                                <  1:208187/71‑1 (MQ=255)
                    gCCAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGtg                                                  <  1:2335407/69‑2 (MQ=255)
                    gCCAGCATGATCATACTTCCGCCCTGCGCTAGCAGTTGATCGACATAAGaa                                                                    >  1:179943/1‑51 (MQ=255)
                     ccAGCATGATCATGCTGCCGCCGTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGtt                                               <  1:5185377/71‑1 (MQ=255)
                     ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatc                                                                  <  1:3114174/52‑1 (MQ=255)
                     ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCAtccg                                                            <  1:5585939/58‑1 (MQ=255)
                     ccAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt                                                <  1:3198675/70‑1 (MQ=255)
                       aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg                                             <  1:3163240/71‑1 (MQ=255)
                       aGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTgg                                             >  1:1936462/1‑71 (MQ=255)
                         cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt                                                <  1:5507567/66‑1 (MQ=255)
                         cATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGc                                           >  1:2295277/1‑71 (MQ=255)
                           tgttCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCa                                         <  1:3217070/68‑1 (MQ=255)
                           tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg                                              >  1:1444583/1‑66 (MQ=255)
                           tGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc                                          <  1:4603298/70‑1 (MQ=255)
                            gATCATACTTCCGCCCTGTGCTTGCAGTTGATCGACATAAGaa                                                                    <  1:1828306/43‑1 (MQ=255)
                            gATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGtt                                               <  1:2871881/64‑1 (MQ=255)
                             aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc                                                             >  1:2968097/1‑49 (MQ=255)
                             aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAatccatcc                                                             >  1:3933305/1‑49 (MQ=255)
                             aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat                                                                   <  1:4334084/43‑1 (MQ=255)
                             aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat                                                                   <  1:430467/43‑1 (MQ=255)
                             aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAat                                                                   <  1:3905659/43‑1 (MQ=255)
                             aTCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGcc                                          >  1:520481/1‑68 (MQ=255)
                              tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg                                                                      <  1:1201293/39‑1 (MQ=255)
                              tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg                                                                      <  1:5372804/39‑1 (MQ=255)
                              tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg                                                                      <  1:1640576/39‑1 (MQ=255)
                              tCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg                                                 >  1:4616898/1‑60 (MQ=255)
                                aTACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAagag                                     >  1:5288592/1‑70 (MQ=255)
                                  aCTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg                                                                      >  1:276670/1‑35 (MQ=255)
                                  aCTTCCGCCCTGCGCTTGCAGTTGATCGACATAAg                                                                      >  1:917123/1‑35 (MQ=255)
                                   ctgccgccgTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAAc                                  >  1:1768185/4‑70 (MQ=255)
                                     tCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCg                                >  1:3303521/1‑70 (MQ=255)
                                      ccgccgTGGGATTGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAAcctg                               <  1:2496049/70‑3 (MQ=255)
                                      ccGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCAGTCCGGCGGTCGTTGGGCCAAGAGAACCg                                >  1:532879/1‑69 (MQ=255)
                                       cGCCCTGCGCTTGCAGTTGATCGACATAAGAatcca                                                                >  1:1688855/1‑36 (MQ=255)
                                       cGCCCTGCGCTTGCAGTTGATCGACATAAGAatcca                                                                >  1:4922593/1‑36 (MQ=255)
                                           cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga                                      >  1:3919429/1‑58 (MQ=255)
                                           cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga                                      >  1:73698/1‑58 (MQ=255)
                                           cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga                                      >  1:3284340/1‑58 (MQ=255)
                                           cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga                                      >  1:5226654/1‑58 (MQ=255)
                                           cTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga                                      >  1:2758279/1‑58 (MQ=255)
                                                 ttGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt                                                <  1:1779207/42‑1 (MQ=255)
                                                 ttGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAAcctg                               <  1:2145841/59‑3 (MQ=255)
                                                  tGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTc                  >  1:1543561/1‑71 (MQ=255)
                                                  tGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAgtga                                    <  1:5144256/53‑1 (MQ=255)
                                                  tGCAGCAGATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAAccgg                   >  1:3406880/1‑68 (MQ=255)
                                                   gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg                                                 <  1:3510167/39‑1 (MQ=255)
                                                   gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCg                                                 <  1:2092348/39‑1 (MQ=255)
                                                   gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt                                                >  1:1098524/1‑40 (MQ=255)
                                                   gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt                                                >  1:1125083/1‑40 (MQ=255)
                                                   gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGt                                                >  1:61690/1‑40 (MQ=255)
                                                   gCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTcc                 >  1:2276558/1‑71 (MQ=255)
                                                       ttGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa                                        <  1:2636206/44‑1 (MQ=255)
                                                       ttGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa                                        <  1:3044223/44‑1 (MQ=255)
                                                        agATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAAccg                    >  1:1166034/2‑62 (MQ=255)
                                                        agATCCACGTAGGAGTCCATACGGCCTGCGGTGGTTGGGCCAAGTGAACCTGATGGATAAcc                     >  1:4609802/2‑62 (MQ=255)
                                                         gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTg                                              >  1:1441623/1‑36 (MQ=255)
                                                         gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCaa                                        >  1:4746208/1‑42 (MQ=255)
                                                         gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAaga                                      >  1:5664825/1‑44 (MQ=255)
                                                         gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAag                                       >  1:63370/1‑43 (MQ=255)
                                                         gATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGt            <  1:2935683/70‑1 (MQ=255)
                                                           tCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGttt          >  1:116620/1‑70 (MQ=255)
                                                              aCATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGc      <  1:2207995/71‑1 (MQ=255)
                                                                aTAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCg    >  1:1045288/1‑71 (MQ=255)
                                                                  aaGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGa  >  1:3023545/1‑71 (MQ=255)
                                                                   |                                                                     
GCTGGCTGCGGTTGCCTTTCGCCAGCATGATCATACTTCCGCCCTGCGCTTGCAGTTGATCGACATAAGAATCCATCCGTCCGGCGGTCGTTGGGCCAAGAGAACCGGAGGCATAACCTTCCGGCGTTTTGGCCGGA  >  Exported/1014664‑1014800

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: