| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,014,861 | C→A | 14.1% | R403L (CGG→CTG) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,014,861 | 0 | C | A | 14.1% | 344.3 / 28.4 | 120 | R403L (CGG→CTG) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base C (52/51); new base A (5/12); total (57/63) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.23e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.70e-01 | |||||||||||
Only 100 of 135 total aligned reads displayed.
TTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGA > Exported/1014792‑1014931 | ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:3490139/1‑71 (MQ=255)ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:2099960/1‑71 (MQ=255)ttGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCg > 1:1742759/1‑71 (MQ=255) cGGACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCtt < 1:386899/71‑1 (MQ=255) ggACCCGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCttt < 1:4819662/71‑1 (MQ=255) cccGCGTAGTAGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAg > 1:488525/1‑71 (MQ=255) cccGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:4683493/1‑71 (MQ=255) tagtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc < 1:5550914/63‑1 (MQ=255) gtaGATCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGt > 1:591435/1‑71 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:2701980/58‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGctct < 1:669181/58‑1 (MQ=255) gtaAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:4561844/1‑63 (MQ=255) taAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt < 1:5614760/70‑1 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGt > 1:2767455/1‑68 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg < 1:3039072/71‑1 (MQ=255) aaTCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:3063084/1‑71 (MQ=255) aTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTgg < 1:571955/64‑1 (MQ=255) aTCGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTgg < 1:1891405/64‑1 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:5657746/54‑1 (MQ=255) tCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTgg < 1:5341876/63‑1 (MQ=255) cGGGTGATCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCaa > 1:76916/1‑71 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:3956383/1‑56 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAg > 1:4045622/1‑56 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa < 1:2865223/70‑1 (MQ=255) ggATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:5541178/71‑1 (MQ=255) atgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAatat < 1:401998/71‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:845235/57‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTg < 1:793295/57‑1 (MQ=255) tgatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATc < 1:3127483/71‑1 (MQ=255) ggatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAata > 1:5177737/2‑69 (MQ=255) gatCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATc > 1:2108383/1‑70 (MQ=255) atCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:3620500/47‑1 (MQ=255) atCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCtt < 1:1608258/47‑1 (MQ=255) atCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAatat < 1:1186710/68‑1 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt > 1:1096361/1‑53 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt > 1:1742229/1‑53 (MQ=255) tCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat < 1:5089026/65‑1 (MQ=255) tCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcg > 1:3013531/1‑71 (MQ=255) tCTTTGATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcg > 1:4376413/1‑71 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:1976161/1‑47 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:3973381/1‑47 (MQ=255) cTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTc > 1:1393894/1‑47 (MQ=255) ttGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc > 1:1243314/1‑71 (MQ=255) tGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcg < 1:1014956/67‑1 (MQ=255) gATGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcggccc < 1:3442358/70‑2 (MQ=255) aTGTACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTcggccca < 1:2628690/70‑3 (MQ=255) tACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACcgacg > 1:2855539/1‑69 (MQ=255) tACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:1044178/71‑1 (MQ=255) tACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt < 1:4260998/71‑1 (MQ=255) tACTGCGGAAGTTCTTTACCGGCGTCAATCAGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGAt < 1:2416040/71‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:2640018/42‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:4970889/42‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGttt < 1:3486280/42‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCaa < 1:3857005/53‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcga > 1:1522583/1‑61 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATaa > 1:4373141/1‑71 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATa < 1:2024512/70‑1 (MQ=255) cTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATa > 1:4221716/1‑70 (MQ=255) gCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGAGTGAGCAat > 1:5339659/1‑52 (MQ=255) cGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCg > 1:4918468/1‑71 (MQ=255) ggCAGCGCTTCACCGTTATCCATCCGCTCTTTCAGt < 1:3237745/36‑1 (MQ=255) ggCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGt < 1:3230830/36‑1 (MQ=255) cAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:5531401/1‑56 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:2042683/61‑1 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg > 1:359039/1‑71 (MQ=255) gCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCg > 1:1479363/1‑71 (MQ=255) gCCCTTCACCGTAATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGAt < 1:5290922/61‑1 (MQ=255) cccTTCACCGTTATCCATCCGCTCTTTCAGTTTgg < 1:1365618/35‑1 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAGTCGTGc > 1:1366287/1‑66 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGc > 1:3192557/1‑66 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt > 1:5052641/1‑70 (MQ=255) cTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGtt > 1:1772105/1‑70 (MQ=255) ttCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgacc < 1:16583/51‑1 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTa < 1:5148124/69‑1 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAg > 1:4027284/1‑71 (MQ=255) tCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCAACGATAATCGTGCCGTTAAg > 1:174298/1‑71 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCTTGAGCAATATCAcgaga > 1:3351006/1‑47 (MQ=39) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAc > 1:2372162/1‑45 (MQ=255) cACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAc > 1:2737524/1‑45 (MQ=255) aCCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAg > 1:5164418/1‑35 (MQ=255) aCCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCg < 1:2110683/71‑1 (MQ=255) ccGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat > 1:4881699/1‑38 (MQ=255) ccGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAat > 1:883362/1‑38 (MQ=255) cGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcg < 1:3509224/43‑1 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:4098501/1‑43 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCAcgac > 1:3658776/1‑43 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATaa < 1:4427120/71‑1 (MQ=255) ttATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATaa < 1:2487399/71‑1 (MQ=255) tATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAg > 1:4278265/1‑71 (MQ=255) tcaatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg < 1:2951692/68‑1 (MQ=255) tcaatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg < 1:792600/68‑1 (MQ=255) tcaatcaGCTCTTTCAGCTTGGCGTGTGCAATATCTCGGCCCACGATAATGGTGCCGGTGAGCGACAAACg < 1:1676986/68‑1 (MQ=255) cacccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgt < 1:5103627/67‑1 (MQ=255) tccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGcc > 1:4689288/1‑52 (MQ=255) tccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGcc > 1:1322693/1‑52 (MQ=255) tccGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCgtg > 1:3549813/1‑69 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAg > 1:933886/1‑70 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:3308024/71‑1 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:2569817/71‑1 (MQ=255) cGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGa < 1:1470266/71‑1 (MQ=255) | TTGGCCGGACCCGCGTAGTAAATCGGATGATCTTTGATGTACTGCGGCAGCCCTTCACCGTTATCCATCCGCTCTTTCAGTTTGGCGTGAGCAATATCACGACCGACGATAATCGTGCCGTTAAGCGATAAGCGTGTAGA > Exported/1014792‑1014931 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |