Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,015,097 G→A 41.3% I324I (ATC→ATT fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,015,0970GA41.3% 63.2 / 112.7 104I324I (ATC→ATTfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base G (36/25);  new base A (21/22);  total (57/47)
Fisher's exact test for biased strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 105 total aligned reads displayed.

CGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGA  >  Exported/1015028‑1015166
                                                                     |                                                                     
cGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa                                                                      <  1:190477/71‑1 (MQ=255)
     tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata                                                                    <  1:4241631/68‑1 (MQ=255)
     tatatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTa                                                                 <  1:1601331/71‑1 (MQ=255)
       tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata                                                                    >  1:3493630/1‑66 (MQ=255)
       tatTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGata                                                                    >  1:685870/1‑66 (MQ=255)
         tttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt                                                             <  1:562367/71‑1 (MQ=255)
          ttGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGt                                                             >  1:5620087/1‑70 (MQ=255)
          tggCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGt                                                             >  1:3042698/3‑70 (MQ=255)
           ggCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTAGCTTTAATGTTACGGt                                                             >  1:5529572/2‑69 (MQ=255)
            gCCTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcag                                                          <  1:493611/71‑1 (MQ=255)
            gCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag                                                          >  1:1544333/1‑71 (MQ=255)
             ccTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcag                                                          <  1:2585167/70‑1 (MQ=255)
              cTGGGTTGTGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTcagcg                                                        <  1:765686/71‑2 (MQ=255)
                ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag                                                       >  1:5459541/1‑70 (MQ=255)
                ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca                                                        >  1:3118813/1‑69 (MQ=255)
                ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca                                                        >  1:2699696/1‑69 (MQ=255)
                ggATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTAGGTcagcag                                                       >  1:2488653/1‑69 (MQ=255)
                 gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa                                                                      >  1:2269201/1‑54 (MQ=255)
                 gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag                                                     >  1:4853656/1‑71 (MQ=255)
                 gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag                                                     <  1:4837800/71‑1 (MQ=255)
                 gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag                                                     >  1:4189154/1‑71 (MQ=255)
                 gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag                                                     >  1:3866169/1‑71 (MQ=255)
                 gATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTAGCTTTGATATTACGGTCagcaga                                                      <  1:489632/70‑1 (MQ=255)
                  aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa                                                                      >  1:2264516/1‑53 (MQ=255)
                  aTTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa                                                                      >  1:5114168/1‑53 (MQ=255)
                   ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagc                                                    <  1:2001343/70‑1 (MQ=255)
                   ttATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagag                                                     >  1:943428/1‑69 (MQ=255)
                    tgtGTTCCAGTTTTTCGATGCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagc                                                    <  1:4692495/67‑1 (MQ=255)
                     gtGTTCCAGTTTTTCGATCCATATACCTTCGCGGTTGATTTTCGCTTTAATGTTACCGTCagcggagcag                                                  <  1:3984026/69‑1 (MQ=255)
                     gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag                                                  <  1:812238/69‑1 (MQ=255)
                     gtGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag                                                  <  1:2617848/69‑1 (MQ=255)
                      tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga                                                <  1:5354398/71‑1 (MQ=255)
                      tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga                                                <  1:1212523/71‑1 (MQ=255)
                      tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga                                                <  1:4598355/71‑1 (MQ=255)
                      tGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga                                                <  1:3690239/71‑1 (MQ=255)
                      tGTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGga                                                <  1:19175/71‑1 (MQ=255)
                       gTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga                                                <  1:2179569/70‑1 (MQ=255)
                       gTTCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGga                                                <  1:1146097/70‑1 (MQ=255)
                        ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga                                              >  1:1604107/1‑71 (MQ=255)
                        ttCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgaga                                              >  1:3603061/1‑71 (MQ=255)
                        ttCCAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga                                              <  1:1525592/71‑1 (MQ=255)
                        ttCCAGTTTTTCGATCCAGATACCTTAGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga                                              <  1:4348262/71‑1 (MQ=255)
                         tCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc                                             >  1:3339198/1‑71 (MQ=255)
                          ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGAc                                             <  1:5284528/70‑1 (MQ=255)
                          ccAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACg                                            >  1:55464/1‑71 (MQ=255)
                          ccAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACg                                            >  1:1805971/1‑71 (MQ=255)
                           cAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagcag                                                  <  1:425225/64‑1 (MQ=255)
                           cAGTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGGCAGCGGAGCAGGAGACGc                                           <  1:4591599/71‑1 (MQ=255)
                            aGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCagcagagca                                                   >  1:2883009/1‑62 (MQ=255)
                             gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag                                                  <  1:3605047/62‑1 (MQ=255)
                             gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag                                                  <  1:1152191/62‑1 (MQ=255)
                             gTTTTTCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCagcggagcag                                                  <  1:988283/62‑1 (MQ=255)
                              tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagcag                                                       <  1:1178992/56‑1 (MQ=255)
                              tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag                                                          <  1:1659209/53‑1 (MQ=255)
                              tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag                                                          <  1:4554022/53‑1 (MQ=255)
                              tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcag                                                          <  1:939032/53‑1 (MQ=255)
                              tttttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGga                                                <  1:3194806/63‑1 (MQ=255)
                               ttttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAatg                                                                    >  1:610987/1‑41 (MQ=16)
                               ttttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAatg                                                                    >  1:3770352/1‑41 (MQ=16)
                                tttCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGa                                                                      >  1:4828660/1‑39 (MQ=255)
                                tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGgaga                                              >  1:1768927/1‑63 (MQ=255)
                                tttCGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCAtg                                      >  1:5106137/1‑70 (MQ=255)
                                   cGATCCAGATACCTTCGGGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCTGGAGACGCCCATgcc                                    <  1:959164/70‑1 (MQ=255)
                                   cGATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgcc                                    >  1:631225/1‑70 (MQ=255)
                                    gATCCAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccga                                  <  1:2196846/71‑1 (MQ=255)
                                       ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca                                                        >  1:1112260/1‑46 (MQ=255)
                                       ccAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca                                                        >  1:1884346/1‑46 (MQ=255)
                                       ccAGATACCTTCGCGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATgccgaccg                               >  1:2889007/1‑71 (MQ=255)
                                          gATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTcagca                                                        <  1:4953286/43‑1 (MQ=255)
                                             ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAg                          >  1:2236504/1‑70 (MQ=255)
                                             ccccTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCACATACCGACCGGGCAg                          >  1:2294138/1‑70 (MQ=255)
                                                cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGCCCGGGCAGGAt                       >  1:5521129/1‑70 (MQ=255)
                                                cTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATg                      >  1:3316979/1‑71 (MQ=255)
                                                 tGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATgc                     >  1:2567914/1‑71 (MQ=255)
                                                  gACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGgag                                               >  1:5123485/1‑44 (MQ=255)
                                                  cgcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAgg                                                 >  1:2607614/3‑42 (MQ=25)
                                                  cgcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAgg                                                 >  1:1368071/3‑42 (MQ=25)
                                                   gcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgcgc                   >  1:678811/2‑71 (MQ=255)
                                                   gcGGTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATgc                     <  1:3486527/68‑1 (MQ=255)
                                                   aCGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGcc                                          <  1:5279903/48‑1 (MQ=255)
                                                     ggTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCg                 <  1:2419461/71‑1 (MQ=255)
                                                      gTTGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt                <  1:1919808/71‑1 (MQ=255)
                                                       ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCa                                        >  1:492989/1‑46 (MQ=255)
                                                       ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCa                                        >  1:2768811/1‑46 (MQ=255)
                                                       ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCa                                        >  1:4669302/1‑46 (MQ=255)
                                                       ttGATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGt                >  1:938789/1‑70 (MQ=255)
                                                       ttGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGt                >  1:4734037/1‑70 (MQ=255)
                                                        tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa              >  1:1126322/1‑71 (MQ=255)
                                                        tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa              >  1:2493085/1‑71 (MQ=255)
                                                        tGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGa              >  1:4244530/1‑71 (MQ=255)
                                                         gATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGAc             >  1:5690256/1‑71 (MQ=255)
                                                         gATTTTCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGAc             >  1:5197910/1‑71 (MQ=255)
                                                          aTCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATaccgaccg                               >  1:4283364/1‑52 (MQ=255)
                                                           ttttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGa                        <  1:1891375/56‑1 (MQ=255)
                                                           ttttCGCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGt           <  1:519775/69‑1 (MQ=255)
                                                               cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCa                                        >  1:226671/1‑38 (MQ=255)
                                                               cGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGt           <  1:2708153/67‑1 (MQ=255)
                                                                gCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGAc             >  1:1503710/1‑64 (MQ=255)
                                                                gCTTTAATGTTACGGTCAGCGGAGCAGGAGACGCCCATGCCGACCGGGCAGGATGCGCCGTGACGTGGCa       <  1:820874/70‑1 (MQ=255)
                                                                     gATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGa  <  1:5118496/70‑1 (MQ=255)
                                                                     |                                                                     
CGGGATATATTTGCCTGGATTATGTTCCAGTTTTTCGATCCAGATCCCCTGACGGTTGATCTTCGCTTTGATATTACGGTCAGCAGAGCAGGAGACGCCCATACCGACCGGGCAGGATGCGCCGTGACGTGGCAGGCGA  >  Exported/1015028‑1015166

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: