Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,015,415 C→G 10.0% T218T (ACG→ACC fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,015,4150CG10.0% 164.3 / 8.0 60T218T (ACG→ACCfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base C (21/33);  new base G (3/3);  total (24/36)
Fisher's exact test for biased strand distribution p-value = 6.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.75e-01

GTTCGTTTCTGCAGAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAT  >  Exported/1015346‑1015482
                                                                     |                                                                   
gTTCGTTTCTGCAGAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCg                                                                    <  1:4111035/71‑1 (MQ=255)
     tttCTGCAGAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGc                                                                     <  1:3543521/65‑1 (MQ=255)
     tttCTGCAGAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGc                                                                     <  1:777738/65‑1 (MQ=255)
     tttCTGCAGAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTgcgc                                                               <  1:727259/71‑1 (MQ=255)
           cAGAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCAtc                                                            <  1:5247040/68‑1 (MQ=255)
             gAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCAtcttct                                                        >  1:3249245/1‑70 (MQ=255)
              aaGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTtctc                                                       >  1:4577754/1‑70 (MQ=255)
                gTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAAc                                                    <  1:2277619/71‑1 (MQ=255)
                     accaATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGt                                               <  1:1023314/71‑1 (MQ=255)
                     accaATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGt                                               <  1:814153/71‑1 (MQ=255)
                          tAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttt                                          <  1:3473114/71‑1 (MQ=255)
                            acgaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt                                        <  1:4536778/71‑1 (MQ=255)
                            acgaacgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt                                        <  1:2967324/71‑1 (MQ=255)
                                 cgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa                                      >  1:794365/1‑68 (MQ=255)
                                 cgCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGtt                                   <  1:5365493/71‑1 (MQ=255)
                                  gCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttt                                          <  1:1857489/63‑1 (MQ=255)
                                  gCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttt                                          <  1:3748241/63‑1 (MQ=255)
                                    gatatGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGtttgc                                <  1:4848153/70‑3 (MQ=255)
                                      tatGGTACGGCGGGCAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTgccg                              >  1:631194/1‑70 (MQ=255)
                                      tatGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAAc                                                    <  1:2984148/49‑1 (MQ=255)
                                      tatGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTcccc                              <  1:4375839/71‑1 (MQ=255)
                                      tatGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTcccc                              >  1:5045484/1‑71 (MQ=255)
                                       atGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCaa                                                     >  1:551921/1‑47 (MQ=255)
                                       atGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAAc                                                    >  1:1898184/1‑48 (MQ=255)
                                        tGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGttt                                  >  1:1269513/1‑65 (MQ=255)
                                           tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa                                      <  1:4972865/58‑1 (MQ=255)
                                           tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                         <  1:4563966/71‑1 (MQ=255)
                                           tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                         <  1:142070/71‑1 (MQ=255)
                                           tACGGAGGACAGGCCGCCGTACCCAGCGTGAGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                         <  1:2622271/71‑1 (MQ=255)
                                             cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTa                                              >  1:3833740/1‑48 (MQ=255)
                                             cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                         >  1:1383487/1‑69 (MQ=255)
                                             cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                         >  1:4328100/1‑69 (MQ=255)
                                               gaggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAg                                                 >  1:3164576/1‑43 (MQ=255)
                                               gaggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAg                      >  1:2282417/1‑70 (MQ=255)
                                                 ggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa                                      <  1:5363181/52‑1 (MQ=255)
                                                 ggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTaa                   >  1:666597/1‑71 (MQ=255)
                                                  ggcAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAg                      <  1:4801432/65‑1 (MQ=255)
                                                  gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt                                        <  1:4864288/49‑1 (MQ=255)
                                                  gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt                                        <  1:799995/49‑1 (MQ=255)
                                                  gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt                                        <  1:1109754/49‑1 (MQ=255)
                                                  gaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt                                        <  1:2588765/49‑1 (MQ=255)
                                                   gcAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGtttg                                 <  1:1713811/54‑2 (MQ=255)
                                                    cAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGc                >  1:4211564/1‑71 (MQ=255)
                                                       gccgccGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTc                        <  1:867798/60‑1 (MQ=255)
                                                         cgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt                                        <  1:3312633/42‑1 (MQ=255)
                                                         cgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa                                      <  1:5326349/44‑1 (MQ=255)
                                                         cgccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCa                                      <  1:1375564/44‑1 (MQ=255)
                                                           ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTaa                                             <  1:1319913/35‑1 (MQ=255)
                                                           ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAAttttt                                        <  1:2836546/40‑1 (MQ=255)
                                                           ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTc                                       <  1:3432428/41‑1 (MQ=255)
                                                           ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGt                                    >  1:2798167/1‑44 (MQ=255)
                                                           ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGt                                    >  1:2249303/1‑44 (MQ=255)
                                                           ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt         >  1:5261989/1‑71 (MQ=255)
                                                           ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt         <  1:3999617/71‑1 (MQ=255)
                                                             gTACCGAGGGTACACATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGATTTGGTTTc       >  1:5678796/1‑71 (MQ=255)
                                                             gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc       >  1:3451956/1‑71 (MQ=255)
                                                             gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc       >  1:3458471/1‑71 (MQ=255)
                                                               aCCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAgca                    >  1:4605434/1‑54 (MQ=255)
                                                                  cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa   >  1:2520491/1‑70 (MQ=255)
                                                                  cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAt  <  1:2068252/71‑1 (MQ=255)
                                                                  cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAt  <  1:2061669/71‑1 (MQ=255)
                                                                   aGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa   >  1:1410421/1‑69 (MQ=255)
                                                                     |                                                                   
GTTCGTTTCTGCAGAAGTTCCACCAATAACGAACGCAATATGATACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAT  >  Exported/1015346‑1015482

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: