Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,015,457 C→A 13.6% T204T (ACG→ACT fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,015,4570CA13.6% 259.0 / 17.7 88T204T (ACG→ACTfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base C (35/41);  new base A (7/5);  total (42/46)
Fisher's exact test for biased strand distribution p-value = 5.39e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

TACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACA  >  Exported/1015389‑1015525
                                                                    |                                                                    
tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                                                                    <  1:4563966/71‑1 (MQ=255)
tACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                                                                    <  1:142070/71‑1 (MQ=255)
tACGGAGGACAGGCCGCCGTACCCAGCGTGAGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                                                                    <  1:2622271/71‑1 (MQ=255)
  cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                                                                    >  1:4328100/1‑69 (MQ=255)
  cGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGt                                                                    >  1:1383487/1‑69 (MQ=255)
    gaggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAg                                                                 >  1:2282417/1‑70 (MQ=255)
      ggaCAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTaa                                                              >  1:666597/1‑71 (MQ=255)
       ggcAGGCTGCAGTACCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAg                                                                 <  1:4801432/65‑1 (MQ=255)
         cAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGc                                                           >  1:4211564/1‑71 (MQ=255)
            gccgccGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTc                                                                   <  1:867798/60‑1 (MQ=255)
                ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt                                                    >  1:5261989/1‑71 (MQ=255)
                ccgTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGtt                                                    <  1:3999617/71‑1 (MQ=255)
                  gTACCGAGGGTACACATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGATTTGGTTTc                                                  >  1:5678796/1‑71 (MQ=255)
                  gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc                                                  >  1:3458471/1‑71 (MQ=255)
                  gTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTc                                                  >  1:3451956/1‑71 (MQ=255)
                    aCCGAGGGTACGCATTTTCTCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAgca                                                               >  1:4605434/1‑54 (MQ=255)
                       cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa                                              >  1:2520491/1‑70 (MQ=255)
                       cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAt                                             <  1:2061669/71‑1 (MQ=255)
                       cAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGAt                                             <  1:2068252/71‑1 (MQ=255)
                        aGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa                                              >  1:1410421/1‑69 (MQ=255)
                           gTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATagag                                         <  1:3478245/71‑1 (MQ=255)
                           gTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATa                                            >  1:1316827/1‑68 (MQ=255)
                            tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTgg                                                      <  1:2885404/57‑1 (MQ=255)
                            tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga                                        <  1:3181154/71‑1 (MQ=255)
                            tGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAgaga                                        <  1:1084939/71‑1 (MQ=255)
                              cgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGAt                                       <  1:4789657/70‑1 (MQ=255)
                              cgcATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATa                                      >  1:5430970/1‑71 (MQ=255)
                                cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAg                                                                 <  1:1173456/42‑1 (MQ=255)
                                cATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc                                     <  1:2189701/70‑1 (MQ=255)
                                 aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt                                   >  1:377443/1‑71 (MQ=255)
                                 aTCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGt                                   >  1:5617337/1‑71 (MQ=255)
                                  ttttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTg                                                       <  1:3261203/48‑1 (MQ=255)
                                  ttttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGt                                                     <  1:4397292/50‑1 (MQ=255)
                                    ttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGtttt                                >  1:399374/1‑71 (MQ=255)
                                    ttctCGACGAGGAAGTTTTTCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTAGTAGAGATACGttt                                 >  1:3581867/1‑68 (MQ=255)
                                    ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa                                              >  1:466936/1‑57 (MQ=255)
                                    ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa                                              >  1:5182584/1‑57 (MQ=255)
                                    ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa                                              >  1:5276588/1‑57 (MQ=255)
                                    ttctCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt                                 >  1:3010719/1‑70 (MQ=255)
                                     tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCATGGCTTTGGTTTCCTGGTAGAGAt                                       <  1:4334658/63‑1 (MQ=255)
                                     tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt                                       <  1:5227161/63‑1 (MQ=255)
                                     tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt                                       <  1:2530501/63‑1 (MQ=255)
                                     tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt                                       <  1:2717369/63‑1 (MQ=255)
                                     tctcGACGAGGAAGTTTTTCAGTTTGCCGGGCGTCAGCAGGGCTTTGGTTTCCTGGTAGAGAt                                       <  1:460578/63‑1 (MQ=255)
                                     tctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTaa                                                              >  1:3294336/1‑40 (MQ=255)
                                     tctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc                                     <  1:4694561/65‑1 (MQ=255)
                                     tctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATAc                                     <  1:5426159/65‑1 (MQ=255)
                                      ctcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCt                                 <  1:4258569/68‑1 (MQ=255)
                                       tcAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATagag                                         >  1:4370668/1‑59 (MQ=255)
                                        cAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCttt                                                        >  1:105406/1‑43 (MQ=255)
                                         aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTaa                                                              >  1:2427887/1‑36 (MQ=255)
                                         aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt                              <  1:3086951/68‑1 (MQ=255)
                                         aaCCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt                              <  1:3157312/68‑1 (MQ=255)
                                         aaCCAGGCAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGt                              <  1:5590001/68‑1 (MQ=255)
                                              ggTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGa                                              <  1:1424968/47‑1 (MQ=255)
                                               gTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAac                     <  1:1417246/71‑1 (MQ=255)
                                               gTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAac                     <  1:4787093/71‑1 (MQ=255)
                                                 aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCt                                                >  1:2734046/1‑42 (MQ=255)
                                                 aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCt                                                >  1:2897564/1‑42 (MQ=255)
                                                 aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGcc                         >  1:625148/1‑65 (MQ=255)
                                                 aaTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAacca                   >  1:3940664/1‑71 (MQ=255)
                                                  aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTg                               <  1:4766448/58‑1 (MQ=255)
                                                  aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccac                  <  1:4047212/71‑1 (MQ=255)
                                                  aTTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccac                  <  1:3980521/71‑1 (MQ=255)
                                                   tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATa                                      >  1:1575859/1‑50 (MQ=255)
                                                   tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATa                                      >  1:5466074/1‑50 (MQ=255)
                                                   tttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATa                                      >  1:3440364/1‑50 (MQ=255)
                                                    ttttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATAAGTTTTGttgg                           >  1:2718277/1‑58 (MQ=255)
                                                    ttttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAaccacc                 <  1:3273283/70‑1 (MQ=255)
                                                     tttCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAgccgccgc               >  1:5472185/1‑69 (MQ=255)
                                                     tttCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTc                                  <  1:4008520/52‑1 (MQ=255)
                                                       tCAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGca                         >  1:2195172/1‑58 (MQ=255)
                                                       tCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGc                          <  1:4831137/58‑1 (MQ=255)
                                                        cAGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCt             <  1:3567305/70‑1 (MQ=255)
                                                         aGTTTGCCGGGAGTCAGCAGGGCTTTGGTTTCCTGGTAGAGATACGTTTTGTTGGCAGAGCCGCCGCCtt            <  1:1191864/70‑1 (MQ=255)
                                                         aGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACg                                    <  1:3921417/46‑1 (MQ=255)
                                                         aGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg                        >  1:4799489/1‑58 (MQ=255)
                                                         aGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg                        >  1:3458132/1‑58 (MQ=255)
                                                          gTTTCCCCAGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTg          >  1:1338241/1‑71 (MQ=255)
                                                             tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGa                       <  1:5494009/55‑1 (MQ=255)
                                                             tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc        <  1:3885495/70‑1 (MQ=255)
                                                             tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc        <  1:3608082/70‑1 (MQ=255)
                                                             tCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGc        >  1:1573830/1‑70 (MQ=255)
                                                               cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa      <  1:1886545/70‑1 (MQ=255)
                                                               cccGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGa      <  1:862705/70‑1 (MQ=255)
                                                                  ggCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACa  <  1:668128/71‑1 (MQ=255)
                                                                   gCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg                        >  1:3268327/1‑48 (MQ=255)
                                                                   gCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCg                        >  1:1130160/1‑48 (MQ=255)
                                                                    |                                                                    
TACGGAGGACAGGCCGCCGTACCCAGCGTGCGCATCTTCTCAACCAGGTAATTTTTCAGTTTCCCCGGCGTCAGTAACGCTTTGGTTTCCTGATAGAGATACGTCTTGTTTGCCGAACCACCACCTTTGGCGATACA  >  Exported/1015389‑1015525

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: