Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,015,682 C→T 25.0% A129A (GCG→GCA) ‡ fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,015,6820CT25.0% 71.1 / 22.1 44A129A (GCG→GCA) ‡fumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base C (23/10);  new base T (3/8);  total (26/18)
Fisher's exact test for biased strand distribution p-value = 3.05e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.73e-01

CCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCT  >  Exported/1015612‑1015752
                                                                      |                                                                      
ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc                                                                        >  1:1071204/1‑71 (MQ=255)
ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc                                                                        >  1:1919440/1‑71 (MQ=255)
ccAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgcc                                                                        >  1:5688093/1‑71 (MQ=255)
 cAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgccg                                                                       >  1:967218/1‑71 (MQ=255)
 cAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGcgccg                                                                       >  1:4531729/1‑71 (MQ=255)
    cggcgCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCtt                                                                    <  1:2243750/71‑1 (MQ=255)
            tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcacca                                                            >  1:327459/1‑71 (MQ=255)
            tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcacca                                                            >  1:163289/1‑71 (MQ=255)
             ttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccac                                                           >  1:4547975/1‑71 (MQ=255)
                   gTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCgg                                                     >  1:1569488/1‑71 (MQ=255)
                       cgcAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCa                                                 <  1:2678046/71‑1 (MQ=255)
                         cAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCaca                                               >  1:5239332/1‑69 (MQ=255)
                         cAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATa                                               >  1:707321/1‑71 (MQ=255)
                           ggttATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCacac                                              >  1:2203505/3‑70 (MQ=255)
                            aTTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTcc                                                  <  1:141177/65‑1 (MQ=255)
                             ttATCCTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcacc                                                             >  1:2000193/1‑53 (MQ=255)
                              tATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACgcgc                                          >  1:2326450/1‑71 (MQ=255)
                               aTCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtca                                                                  >  1:4562658/1‑46 (MQ=255)
                                tCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTg                                        >  1:3654795/1‑71 (MQ=255)
                                 cTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATcaccgc                                                           <  1:963907/51‑3 (MQ=255)
                                 cTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccaccac                                                        <  1:2301525/54‑1 (MQ=255)
                                     gATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCtt                                   <  1:1704682/71‑1 (MQ=255)
                                     gATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtcat                                                                 >  1:4994944/1‑41 (MQ=255)
                                     gATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTtcat                                                                 >  1:5581721/1‑41 (MQ=255)
                                     gATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCAt                                                >  1:1070353/1‑58 (MQ=255)
                                      atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt                                  <  1:1647556/71‑1 (MQ=255)
                                      atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt                                  <  1:1357801/71‑1 (MQ=255)
                                      atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt                                  <  1:2118962/71‑1 (MQ=255)
                                       tataGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt                                  <  1:1981250/70‑1 (MQ=255)
                                        ataAGTGTTATAGACACCGCGCGCCAGCGCCGCTCCATCACCACCACCGGTCCATACACGCTGCCCttttt                                >  1:4085079/1‑71 (MQ=255)
                                         taAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCa                                                 <  1:4237096/53‑1 (MQ=255)
                                           aGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTAc                             >  1:2649153/1‑71 (MQ=255)
                                            gtgtTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTAcc                            <  1:5075048/71‑1 (MQ=255)
                                                 ataGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaaca                        >  1:5127351/1‑70 (MQ=255)
                                                  taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaacaa                       >  1:3402511/1‑70 (MQ=255)
                                                  taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaacaa                       >  1:2528372/1‑70 (MQ=255)
                                                  taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat                      <  1:4727397/71‑1 (MQ=255)
                                                    gACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaataa                    >  1:3811583/1‑71 (MQ=255)
                                                     acacCGCGCGCCAGCGCCGCTTCATCACCACCCCCGGTCCATACCCGCTGCCCTTTTTTACCAACaataat                   >  1:520643/1‑71 (MQ=255)
                                                      cacCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc                  <  1:3447993/71‑1 (MQ=255)
                                                       acCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg                 <  1:2695996/71‑1 (MQ=255)
                                                        ccGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcgc                >  1:3982361/1‑71 (MQ=255)
                                                         cgcgcgCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg                 <  1:3658047/69‑1 (MQ=255)
                                                          gcgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCgg              >  1:232508/1‑71 (MQ=255)
                                                           cgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATa                                               >  1:435346/1‑37 (MQ=255)
                                                           cgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCttttt                                >  1:5052715/1‑52 (MQ=255)
                                                              gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGccc                                     <  1:3002665/44‑1 (MQ=255)
                                                               ccAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGccc                                     <  1:4759638/43‑1 (MQ=255)
                                                                 aGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg        >  1:1551041/1‑70 (MQ=255)
                                                                   cgccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt     >  1:4722473/1‑71 (MQ=255)
                                                                    gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat                      >  1:5430502/1‑53 (MQ=255)
                                                                    gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat                      >  1:1775721/1‑53 (MQ=255)
                                                                    gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc                  >  1:4629508/1‑57 (MQ=255)
                                                                     ccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATc    <  1:1063355/70‑1 (MQ=255)
                                                                      cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt  <  1:3896823/71‑1 (MQ=255)
                                                                      cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt  <  1:3515801/71‑1 (MQ=255)
                                                                      |                                                                      
CCAGCGGCGCGTTTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCT  >  Exported/1015612‑1015752

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: