| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 1,015,694 | A→G | 28.6% | G125G (GGT→GGC) | fumA ← | aerobic Class I fumarate hydratase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 1,015,694 | 0 | A | G | 28.6% | 72.3 / 36.7 | 56 | G125G (GGT→GGC) | fumA | aerobic Class I fumarate hydratase |
| Reads supporting (aligned to +/- strand): ref base A (23/17); new base G (5/11); total (28/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.38e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.51e-01 | |||||||||||
TTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGT > Exported/1015624‑1015759 | tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcacca > 1:327459/1‑71 (MQ=255)tttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcacca > 1:163289/1‑71 (MQ=255) ttGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccac > 1:4547975/1‑71 (MQ=255) gTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCgg > 1:1569488/1‑71 (MQ=255) cgcAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCa < 1:2678046/71‑1 (MQ=255) cAGGTTATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCaca > 1:5239332/1‑69 (MQ=255) cAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATa > 1:707321/1‑71 (MQ=255) ggttATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCacac > 1:2203505/3‑70 (MQ=255) aTTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTcc < 1:141177/65‑1 (MQ=255) tATCTTCGATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACgcgc > 1:2326450/1‑71 (MQ=255) tCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTg > 1:3654795/1‑71 (MQ=255) cTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATcaccaccac < 1:2301525/54‑1 (MQ=255) gATATAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCtt < 1:1704682/71‑1 (MQ=255) gATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCAt > 1:1070353/1‑58 (MQ=255) atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:2118962/71‑1 (MQ=255) atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:1647556/71‑1 (MQ=255) atatAGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:1357801/71‑1 (MQ=255) tataGGTGTTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCttt < 1:1981250/70‑1 (MQ=255) ataAGTGTTATAGACACCGCGCGCCAGCGCCGCTCCATCACCACCACCGGTCCATACACGCTGCCCttttt > 1:4085079/1‑71 (MQ=255) taAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCa < 1:4237096/53‑1 (MQ=255) aGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTAc > 1:2649153/1‑71 (MQ=255) gtgtTATAGACGCCTTTCGACAGCGTTTCTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTAcc < 1:5075048/71‑1 (MQ=255) ataGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaaca > 1:5127351/1‑70 (MQ=255) taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaacaa > 1:2528372/1‑70 (MQ=255) taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACcaacaa > 1:3402511/1‑70 (MQ=255) taGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat < 1:4727397/71‑1 (MQ=255) gACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaataa > 1:3811583/1‑71 (MQ=255) acacCGCGCGCCAGCGCCGCTTCATCACCACCCCCGGTCCATACCCGCTGCCCTTTTTTACCAACaataat > 1:520643/1‑71 (MQ=255) cacCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc < 1:3447993/71‑1 (MQ=255) acCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg < 1:2695996/71‑1 (MQ=255) ccGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcgc > 1:3982361/1‑71 (MQ=255) cgcgcgCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg < 1:3658047/69‑1 (MQ=255) gcgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCgg > 1:232508/1‑71 (MQ=255) cgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATa > 1:435346/1‑37 (MQ=255) cgcgcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCttttt > 1:5052715/1‑52 (MQ=255) gcCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGccc < 1:3002665/44‑1 (MQ=255) ccAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGccc < 1:4759638/43‑1 (MQ=255) aGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg > 1:1551041/1‑70 (MQ=255) cgccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTAt > 1:4722473/1‑71 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat > 1:5430502/1‑53 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACaat > 1:1775721/1‑53 (MQ=255) gccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc > 1:4629508/1‑57 (MQ=255) ccgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATc < 1:1063355/70‑1 (MQ=255) cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt < 1:3515801/71‑1 (MQ=255) cgcTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCt < 1:3896823/71‑1 (MQ=255) tcTTCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACcgac > 1:1429097/2‑47 (MQ=16) cTTCATCACCACCACCGGTCCATACACGCTGCCCtt < 1:623383/36‑1 (MQ=255) cTTCATCACCACCACCGGTCCATACACGCTGCCCtt < 1:5502752/36‑1 (MQ=255) cTTCATCACCACCACCGGTCCATACACGCTGCCCtt < 1:5361848/36‑1 (MQ=255) ttCATCACCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTg < 1:1524142/69‑1 (MQ=255) tcatcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCgg < 1:237579/61‑1 (MQ=255) catcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTggc < 1:2661484/69‑3 (MQ=255) catcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGAGGTGCCGGTATCCTgtc < 1:2363479/69‑3 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCgg > 1:2489002/1‑53 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:3467340/1‑67 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa > 1:5142457/1‑67 (MQ=255) atcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAgg < 1:770359/71‑1 (MQ=255) tcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGt < 1:243615/71‑1 (MQ=255) tcaCCGCCGCCGGTCCACACGCGCTGGCCTTTTTTACCGACGATGATCGCGGTGCCGGTATCCTGGCAGGt > 1:4035952/1‑71 (MQ=255) tcaCCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGa < 1:646429/66‑1 (MQ=255) accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACaataat > 1:4356031/1‑45 (MQ=255) accaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATcg > 1:1572158/1‑47 (MQ=255) ccaccaccGGTCCATACACGCTGCCCTTTTTTACCAACaata < 1:431388/42‑1 (MQ=255) ccaccaccGGTCCATACACGCTGCCCTTTTTTACCAACAATAATc < 1:1340906/45‑1 (MQ=255) | TTTGCGAGTAGCGCAGATTATCTTCGATATAAGTGTTATAGACACCGCGCGCCAGCGCCGCTTCATCACCACCACCGGTCCATACACGCTGCCCTTTTTTACCAACAATAATCGCGGTGCCGGTATCCTGACAGGT > Exported/1015624‑1015759 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |