Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,015,823 A→G 11.3% N82N (AAT→AAC fumA ← aerobic Class I fumarate hydratase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,015,8230AG11.3% 251.2 / 19.0 97N82N (AAT→AACfumAaerobic Class I fumarate hydratase
Reads supporting (aligned to +/- strand):  ref base A (56/30);  new base G (2/9);  total (58/39)
Fisher's exact test for biased strand distribution p-value = 6.20e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.40e-01

ACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAAC  >  Exported/1015754‑1015892
                                                                     |                                                                     
aCAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt                                                                      >  1:1035501/1‑71 (MQ=255)
aCAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt                                                                      >  1:4058642/1‑71 (MQ=255)
 cAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt                                                                     >  1:5096043/1‑71 (MQ=255)
 cAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt                                                                     <  1:4048499/71‑1 (MQ=255)
    gtcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTc                                                                  <  1:184778/68‑1 (MQ=255)
     tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg                                                                 <  1:800685/69‑1 (MQ=255)
     tcggCAGCACGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCg                                                                 <  1:4983230/69‑1 (MQ=255)
       ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                                                <  1:1965448/70‑1 (MQ=255)
       ggCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt                                                               >  1:3020013/1‑71 (MQ=255)
         cAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTgg                                                             >  1:1444056/1‑71 (MQ=255)
         cAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTgg                                                             >  1:389951/1‑71 (MQ=255)
          aGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc                                                            >  1:2746248/1‑71 (MQ=255)
           gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc                                                           >  1:978119/1‑71 (MQ=255)
           gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc                                                           >  1:4326027/1‑71 (MQ=255)
           gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc                                                           >  1:4096225/1‑71 (MQ=255)
           gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGcc                                                           >  1:321512/1‑71 (MQ=255)
           gAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc                                                            >  1:334766/1‑70 (MQ=255)
            aaCGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCt                                                          <  1:2604459/71‑1 (MQ=255)
             aCGCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCt                                                               >  1:3612019/1‑65 (MQ=255)
              cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc                                                        >  1:4245253/1‑71 (MQ=255)
              cGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTcc                                                        >  1:2402841/1‑71 (MQ=255)
               gCCTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGtttt                                                                   <  1:4648872/59‑1 (MQ=255)
                ccTTTGGCGGCGATTTCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTctg                                                       >  1:3510957/1‑68 (MQ=255)
                  tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTc                                                                  <  1:1010751/57‑1 (MQ=255)
                  tttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTc                                                                  <  1:1200733/57‑1 (MQ=255)
                   ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGt                                                    >  1:2318724/1‑70 (MQ=255)
                   ttCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTACGGGt                                                    >  1:5195157/1‑70 (MQ=255)
                    tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                                                >  1:3601394/1‑57 (MQ=255)
                    tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                                                >  1:2722800/1‑57 (MQ=255)
                    tCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                                                >  1:3277650/1‑57 (MQ=255)
                        cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg                                                >  1:5350317/1‑69 (MQ=255)
                        cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg                                                >  1:53878/1‑69 (MQ=255)
                        cgcgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACg                                                >  1:5304385/1‑69 (MQ=255)
                          cgATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGa                                            <  1:3181149/71‑1 (MQ=255)
                           gATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtt                                                                     <  1:3794902/45‑1 (MQ=255)
                            atttCGGAGTTTCTTAAGAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAAt                                          <  1:5408500/68‑1 (MQ=255)
                            aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAtttt                                                                   >  1:3358815/1‑46 (MQ=255)
                            aTGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCAt                                                                      <  1:1164286/43‑1 (MQ=255)
                             tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCgg                                                      >  1:4340196/1‑58 (MQ=255)
                             tGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg                                         <  1:5305721/71‑1 (MQ=255)
                              gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGc                                               >  1:3968666/1‑64 (MQ=255)
                              gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt                                        >  1:780844/1‑71 (MQ=255)
                              gTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt                                        >  1:1802020/1‑71 (MQ=255)
                                 ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc                                                            >  1:3472034/1‑48 (MQ=255)
                                 ggAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGc                                                            >  1:4184880/1‑48 (MQ=255)
                                     ttACGCAGGAATTGCAGCGCCACATATTTATCATTTTc                                                                  <  1:1534111/38‑1 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                                                 >  1:3471870/1‑37 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                                                 >  1:806016/1‑37 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCg                                                                 >  1:3437760/1‑37 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                                                >  1:3403599/1‑38 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGc                                                                >  1:1129622/1‑38 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCttg                                                             >  1:507482/1‑39 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt                                                               >  1:5571374/1‑39 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCt                                                               >  1:1777286/1‑39 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                                                       >  1:3334255/1‑47 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                                                       >  1:3739970/1‑47 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                                                       >  1:1514323/1‑47 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                                                       >  1:5484388/1‑47 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCg                                                       >  1:1097490/1‑47 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAcc                                <  1:429491/70‑1 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCAc                                 >  1:1528072/1‑69 (MQ=255)
                                       aCGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCt                               >  1:5421536/1‑71 (MQ=255)
                                        cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGTTCACGCAGAATg                                         <  1:3467043/60‑1 (MQ=255)
                                        cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAGTg                                         <  1:2447274/60‑1 (MQ=255)
                                        cGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATg                                         <  1:4853194/60‑1 (MQ=255)
                                           aagAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgt                             <  1:4656579/67‑1 (MQ=255)
                                           aGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttg                           <  1:4675294/71‑1 (MQ=255)
                                            ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt                          >  1:4551879/1‑71 (MQ=255)
                                            ggAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt                          >  1:4422276/1‑71 (MQ=255)
                                             gAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgtttct                         <  1:454642/71‑4 (MQ=255)
                                             gAATTGCAGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCtgtttct                         <  1:5373429/71‑4 (MQ=255)
                                             gAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTgttgtt                         <  1:1863648/71‑1 (MQ=255)
                                             gAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTgttgtt                         <  1:3149309/71‑1 (MQ=255)
                                              aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGaa                                           >  1:1826152/1‑52 (MQ=255)
                                              aaTTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGttgttg                        <  1:1931121/71‑1 (MQ=255)
                                                ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt                                        <  1:4871374/53‑1 (MQ=255)
                                                ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGt                                        <  1:1562051/53‑1 (MQ=255)
                                                ttGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTgttgtt                         <  1:5392302/68‑1 (MQ=255)
                                                  gCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgc                    >  1:875784/1‑71 (MQ=255)
                                                   cAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTgcg                   <  1:396762/71‑1 (MQ=255)
                                                    aGCGCCACGTACTTGTCGTTTTCGCTGGCTTCTGGATCGTGAAGAATAGCCGCAACCTGTTTCTGGTgtgc                  <  1:5010150/71‑1 (MQ=255)
                                                         cacaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgtt                            <  1:3193465/56‑1 (MQ=255)
                                                          acaTATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTgg                       <  1:4727920/60‑1 (MQ=255)
                                                            atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGcc                                   <  1:4517514/46‑1 (MQ=255)
                                                            atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt                          <  1:5620737/55‑1 (MQ=255)
                                                            atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGt                      >  1:1850817/1‑59 (MQ=255)
                                                            atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGt                      >  1:1200501/1‑59 (MQ=255)
                                                            atatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTg                     >  1:2582099/1‑60 (MQ=255)
                                                             tatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag         >  1:4616315/1‑71 (MQ=255)
                                                             tatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag         >  1:2084705/1‑71 (MQ=255)
                                                             tatTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcag         >  1:2288621/1‑71 (MQ=255)
                                                               tttATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACgcagc        <  1:1409482/70‑1 (MQ=255)
                                                                 tATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCtgttgt                          >  1:2550882/1‑50 (MQ=255)
                                                                   tCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTgg                       <  1:677568/51‑1 (MQ=255)
                                                                   tCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGa    <  1:4186115/70‑1 (MQ=255)
                                                                    cATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGaa   <  1:1095304/70‑1 (MQ=255)
                                                                    cATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac  >  1:5410106/1‑71 (MQ=255)
                                                                     aTTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAac  <  1:4840600/70‑1 (MQ=255)
                                                                     |                                                                     
ACAGGTTGGCAGAACGCCTTTCGCCGCGATGTCGGAGTTACGCAGGAATTGCAGCGCCACATATTTATCATTTTCGCTGGCCTCCGGGTCACGCAGAATGTCGGCCACCTGTTGTTGGTGCGCCGGACGCAGCATGAAC  >  Exported/1015754‑1015892

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: