Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,019,201 G→A 55.4% intergenic (+150/+76) ydgA → / ← uidC conserved hypothetical protein/predicted outer membrane porin protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,019,2010GA55.4% 27.1 / 87.4 74intergenic (+150/+76)ydgA/uidCconserved hypothetical protein/predicted outer membrane porin protein
Reads supporting (aligned to +/- strand):  ref base G (14/19);  new base A (19/22);  total (33/41)
Fisher's exact test for biased strand distribution p-value = 8.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.14e-01

CAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTC  >  Exported/1019136‑1019262
                                                                 |                                                              
cAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCa                                                           >  1:1883038/1‑71 (MQ=255)
 aGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCa                                                           <  1:3862038/70‑1 (MQ=255)
  ggCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATc                                                         <  1:375461/71‑1 (MQ=255)
  ggCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATc                                                         <  1:2845017/71‑1 (MQ=255)
  ctatgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATc                                                         <  1:4228679‑M2/14‑1 (MQ=255)
  ctatgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATc                                                         <  1:1437379‑M2/14‑1 (MQ=255)
   tatgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATcc                                                        >  1:2101907‑M2/57‑71 (MQ=255)
   tatgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATcc                                                        >  1:1903227‑M2/57‑71 (MQ=255)
   tatgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATcc                                                        >  1:1004805‑M2/57‑71 (MQ=255)
    atgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATc                                                         >  1:1415165‑M2/56‑69 (MQ=255)
    atgcctgatgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATc                                                         >  1:2946535‑M2/56‑69 (MQ=255)
     cTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCgg                                                      >  1:2754390/1‑71 (MQ=255)
      tACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcgtcgc                                                            <  1:4775957/64‑1 (MQ=255)
      tACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTAcgccgc                                                            <  1:3032851/64‑1 (MQ=255)
       aCCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCa                                                    >  1:4171515/1‑71 (MQ=255)
         cTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATa                                                  <  1:2408845/71‑1 (MQ=255)
          gatgcgacgctgaagcgtcttatcaagcctacatagcactgccacgtatGTTTACACCGCATCCGGCATa                                                  >  1:818729‑M2/50‑70 (MQ=255)
           atgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaa                                                 <  1:713490‑M2/22‑1 (MQ=255)
            tgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaa                                                 <  1:2048094‑M2/22‑1 (MQ=255)
            tgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaa                                                 <  1:2484138‑M2/22‑1 (MQ=255)
            tgcgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaa                                                 <  1:4048171‑M2/22‑1 (MQ=255)
             tCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATaaaa                                               <  1:1845442/70‑1 (MQ=255)
              cgacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCAtaaaaac                                             <  1:274881‑M2/26‑2 (MQ=255)
              ccTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATaaaa                                               <  1:4027220/69‑1 (MQ=255)
               gacgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCataaaaaca                                            >  1:1857692‑M2/45‑69 (MQ=255)
               cTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAc                                           <  1:5511288/71‑1 (MQ=255)
               cTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAAc                                           <  1:1111320/71‑1 (MQ=255)
                acgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGcataaaaacac                                           <  1:2096420‑M2/28‑4 (MQ=255)
                acgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaaaa                                              <  1:3098024‑M2/25‑1 (MQ=255)
                acgctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaaaa                                              <  1:1281470‑M2/25‑1 (MQ=255)
                   ctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaa                                                <  1:1279892‑M2/23‑1 (MQ=255)
                   ctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaa                                                <  1:2391419‑M2/23‑1 (MQ=255)
                   ctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACAcgcg                                        <  1:3770558‑M2/31‑1 (MQ=255)
                   ctgaagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACAcgcg                                        <  1:4858968‑M2/31‑1 (MQ=255)
                    acacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCa                                      >  1:1026075/1‑71 (MQ=255)
                     cacTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCAc                                     <  1:829182/71‑1 (MQ=255)
                      aagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCa                                      <  1:4692444‑M2/33‑1 (MQ=255)
                      aagcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCAc                                     >  1:774384‑M2/38‑71 (MQ=255)
                       cTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCa                                                           <  1:1365159/48‑1 (MQ=255)
                       cTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCa                                                           <  1:4097776/48‑1 (MQ=255)
                       agcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCAc                                     <  1:3150977‑M2/34‑1 (MQ=255)
                        tttGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACttt                                  >  1:3941050/1‑71 (MQ=255)
                        gcgtcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACtt                                   >  1:1562269‑M2/36‑71 (MQ=255)
                         ttGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCa                                                           >  1:2617432/1‑46 (MQ=255)
                          tGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATaaaa                                               <  1:3349831/57‑1 (MQ=255)
                          tGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTg                                 <  1:1034573/70‑1 (MQ=255)
                           tcttatcatgcctacatagcactgccacgtatGTTTTCACCGCATCCGGCAtat                                                 <  1:208239‑M2/22‑2 (MQ=255)
                           tcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaa                                                <  1:5660959‑M2/23‑1 (MQ=255)
                           tcttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATaaa                                                <  1:1078565‑M2/23‑1 (MQ=255)
                            cttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACTTTg                                 >  1:4855398‑M2/32‑69 (MQ=255)
                             ttatcatgcctacatagcactgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACtttgcta                              >  1:5651377‑M2/31‑68 (MQ=255)
                                tATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTcaacaa                          >  1:4326407/1‑71 (MQ=255)
                                    aGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACtt                                   <  1:687423/58‑1 (MQ=255)
                                    aGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCt                       >  1:5362425/1‑70 (MQ=255)
                                     gTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGa                     <  1:5690720/71‑1 (MQ=255)
                                      tttttGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTg                                 <  1:3932357/58‑1 (MQ=255)
                                         catagcactgcaacgtatGTTTACACCGCATCCGGCataaaaaca                                            >  1:194876‑M2/19‑43 (MQ=255)
                                          atagcactgccacgtatGTTTACACCGCATCCGGCATaaa                                                <  1:5174100‑M2/23‑1 (MQ=255)
                                          atagcactgccacgtatGTTTACACCGCATCCGGCATaaa                                                <  1:1356576‑M2/23‑1 (MQ=255)
                                           tagcactgccacgtatGTTTACACCGCATCCGGCATaaaaa                                              >  1:4103816‑M2/17‑41 (MQ=255)
                                            agcactgccacgtatGTTTACACCGCATCCGGCATaaaaa                                              >  1:781077‑M2/16‑40 (MQ=255)
                                            agcactgccacgtatGTTTACACCGCATCCGGCATaaaaa                                              >  1:1438687‑M2/16‑40 (MQ=255)
                                            agcactgccacgtatGTTTACACCGCATCCGGCATaaaaa                                              >  1:1712395‑M2/16‑40 (MQ=255)
                                            agcactgccacgtatGTTTACACCGCATCCGGCATaaaaa                                              >  1:462448‑M2/16‑40 (MQ=255)
                                             gcactgccacgtatGTTTACACCGCATCCGGCataaaaaca                                            >  1:2988740‑M2/15‑39 (MQ=255)
                                             gcactgccacgtatGTTTACACCGCATCCGGCataaaaaca                                            >  1:1837029‑M2/15‑39 (MQ=255)
                                                ctgccacgtatGTTTACACCGCATCCGGCATAAAAACACGCGCACTTtgc                                <  1:2442155‑M2/39‑2 (MQ=255)
                                                   gATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGAttt       >  1:4011657/1‑71 (MQ=255)
                                                       aGGCGTTTACGCCGCATCCGTCATAAAAAACGCGCACTTTGTCAACAATCTGa                     <  1:5426096/53‑1 (MQ=255)
                                                        ggCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTtctc  <  1:1972496/71‑1 (MQ=255)
                                                             ttACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGaa                    <  1:4598763/48‑1 (MQ=255)
                                                              tACGCCGCATCCGGCATAAACAACGCGCACTTTGTCAGCAAt                         >  1:1310397/1‑42 (MQ=11)
                                                              tACGCCGCATCCGGCATAAACAACGCGCACTTTGTCAGCAATCTGa                     >  1:1228585/1‑46 (MQ=11)
                                                              tACGCCGCATCCGGCATAAACAACGCGCACTTTGTCAGCAATCTGa                     >  1:1513414/1‑46 (MQ=11)
                                                              tACGCCGCATCCGGCATAAACAACGCGCACTTTGTCAGCAATCTGa                     >  1:1292796/1‑46 (MQ=11)
                                                              tACGCCGCATCCGGCATAAACAACGCGCACTTTGTCAGCAATCTGa                     >  1:222252/1‑46 (MQ=11)
                                                                 gccgcATCCGGCATAAAAAACGCGCACTTTGTCAACAATCt                       <  1:1631437/41‑1 (MQ=255)
                                                                 |                                                              
CAGGCCTACCTTTTCCTGCAACACTTTGAATTTATGAGTTTTTGTAGGCTGGATAAGGCGTTTACGCCGCATCCGGCATAAAAAACGCGCACTTTGTCAACAATCTGAAACGCCGGAGATTTTTCTC  >  Exported/1019136‑1019262

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: