Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,191,560	T→C	69.8%	*116W (TGA→TGG)	rnd ←	ribonuclease D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,191,560	0	T	C	69.8%	101.0 / 54.2	76	*116W (TGA→TGG)	rnd	ribonuclease D
Reads supporting (aligned to +/- strand):  ref base T (13/10);  new base C (29/24);  total (42/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

AGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCA  >  Exported/1191493‑1191629                                                                    |                                                                      agTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCAt                                                                    <  1:1695743/71‑1 (MQ=255) agTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCAt                                                                    <  1:1286880/71‑1 (MQ=255)     ggTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACa                                                                <  1:1116261/71‑1 (MQ=255)     ggTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACa                                                                >  1:3435791/1‑71 (MQ=255)      gtgtGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACAt                                                               <  1:3979065/71‑1 (MQ=255)      gTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACAt                                                               <  1:2902803/71‑1 (MQ=255)       tGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACAt                                                               <  1:5683018/70‑1 (MQ=255)        gcgcGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCg                                                             >  1:5558734/1‑71 (MQ=255)         cgcgATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCgg                                                            <  1:694846/71‑1 (MQ=255)         cgcgATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCgg                                                            >  1:3338439/1‑71 (MQ=255)            gATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCg                                                         >  1:5660801/1‑71 (MQ=255)            gATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCg                                                         >  1:5055130/1‑71 (MQ=255)             aTTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGt                                                        >  1:2609941/1‑71 (MQ=255)             aTTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGt                                                        >  1:2189164/1‑71 (MQ=255)             aTTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGt                                                        >  1:4860554/1‑71 (MQ=255)              ttCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTc                                                       >  1:5271823/1‑71 (MQ=255)               tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTcc                                                      >  1:3696132/1‑71 (MQ=255)               tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGa                                                                  >  1:327485/1‑59 (MQ=255)               tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGa                                                                  >  1:305567/1‑59 (MQ=255)               tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTcc                                                      >  1:3557863/1‑71 (MQ=255)               tCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTcc                                                      >  1:1595948/1‑71 (MQ=255)                cACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCg                                                     >  1:1966608/1‑71 (MQ=255)                 aCTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCg                                                     >  1:885320/1‑70 (MQ=255)                    ctTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAg                                                  >  1:3705913/1‑70 (MQ=255)                       gTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCg                                                         >  1:1779109/1‑60 (MQ=255)                       gTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa                                                 <  1:1913818/68‑1 (MQ=255)                       gTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa                                                 <  1:3630306/68‑1 (MQ=255)                       gTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa                                                 <  1:1248167/68‑1 (MQ=255)                        tCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGgc                                             <  1:4957590/71‑1 (MQ=255)                         ccAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAg                                                  <  1:3247608/65‑1 (MQ=255)                           aGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGgcagc                                          <  1:385931/71‑1 (MQ=255)                             cGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGgcagc                                          >  1:2093728/1‑69 (MQ=255)                              gTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAg                                       <  1:5166510/71‑1 (MQ=255)                              gTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAg                                       >  1:5430549/1‑71 (MQ=255)                              gTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAg                                       >  1:2734450/1‑71 (MQ=255)                                 aCGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCa                                                                     >  1:3141957/1‑38 (MQ=255)                                 aCGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCa                                                   >  1:4276948/1‑56 (MQ=255)                                 aCGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGa                                     <  1:2336051/70‑1 (MQ=255)                                 aCGCCGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGAt                                    >  1:3874343/1‑71 (MQ=255)                                    ccGGAATACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg                                 <  1:5148212/71‑1 (MQ=255)                                        aaTACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAg                                                  <  1:2196711/50‑1 (MQ=255)                                          tACTCTTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtc                           <  1:3846381/71‑1 (MQ=255)                                            ctctTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgtg                             <  1:5388374/67‑1 (MQ=255)                                            ctctTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtcaa                         >  1:4890908/1‑71 (MQ=255)                                            ctctTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtca                          <  1:5055779/70‑1 (MQ=255)                                             tctTCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCgtg                             <  1:4542072/66‑1 (MQ=255)                                               ttCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGc                                <  1:5054368/61‑1 (MQ=255)                                                tCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa                                                 <  1:2020294/43‑1 (MQ=255)                                                tCCACCATGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGa                                                 <  1:1651101/43‑1 (MQ=255)                                                 ccaccaTGGAAGCGAAACCCCATGACATCGGGCGTTCGCAg                                                  >  1:124873/1‑41 (MQ=38)                                                  caccaTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGTGTGTCAATCAAg                    <  1:5235365/70‑1 (MQ=255)                                                   accaTGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAggg                  >  1:1039116/1‑71 (MQ=255)                                                    ccaTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGtc                           <  1:4147045/61‑1 (MQ=255)                                                    ccaTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGt                 <  1:2197162/71‑1 (MQ=255)                                                    ccaTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGt                 <  1:372372/71‑1 (MQ=255)                                                     caTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGtt                <  1:5414120/71‑1 (MQ=255)                                                      aTGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTtg               >  1:1244167/1‑71 (MQ=255)                                                       tGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGc                                <  1:4956068/53‑1 (MQ=255)                                                       tGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGc                                <  1:4962434/53‑1 (MQ=255)                                                       tGGAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAgg                                      <  1:3096740/47‑1 (MQ=255)                                                        ggAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGc                                <  1:1310722/52‑1 (MQ=255)                                                         gAAGCGAAACCCCATGACATCGGGCGTCCGCAGaa                                                >  1:445553/1‑35 (MQ=255)                                                         gAAGCGAAACCCCATGACATCGGGCGTCCGCAGaa                                                >  1:4091739/1‑35 (MQ=255)                                                         gAAGCGAAACCCCATGACATCGGGCGTCCGCAGaa                                                >  1:1961488/1‑35 (MQ=255)                                                         gAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTgg            >  1:5073327/1‑71 (MQ=255)                                                         gAAGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTgg            >  1:5068180/1‑71 (MQ=255)                                                           aGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGAtt                                   >  1:1495634/1‑46 (MQ=255)                                                           aGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg                                 >  1:374586/1‑48 (MQ=255)                                                           aGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg                                 >  1:5247547/1‑48 (MQ=255)                                                           aGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg                                 >  1:3264401/1‑48 (MQ=255)                                                           aGCGAAACCTCAAGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTg                                 >  1:48360/1‑48 (MQ=255)                                                           aGCGAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGt           >  1:4701558/1‑70 (MQ=255)                                                              gAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAgg                                      >  1:3840118/1‑40 (MQ=255)                                                              gAAACCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAgg                                      >  1:2360993/1‑40 (MQ=255)                                                                 aCCCCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGc    >  1:1830921/1‑71 (MQ=255)                                                                   cTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCa  <  1:2128028/71‑1 (MQ=255)                                                                    |                                                                      AGTCGGTGCGCGATTCACTCTTGTCCAGCGTAACGCCGGAATACTCTTCCACCATGGAAGCGAAACCTCATGACATCGGGCGTCCGCAGAAGGCAGCAAGGATTTGCGTGTCAATCAAGGGTTGTGGTAATTCGCCA  >  Exported/1191493‑1191629

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑