BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	Exported	1,201,060	A→G	14.3%	A71A (GCA→GCG)	sdaA →	L‑serine deaminase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	Exported	1,201,060	0	A	G	14.3%	301.5 / 35.5	126	A71A (GCA→GCG)	sdaA	L‑serine deaminase I
Reads supporting (aligned to +/- strand): ref base A (52/56); new base G (7/11); total (59/67)
Fisher's exact test for biased strand distribution p-value = 6.11e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 126 total aligned reads displayed.

CGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCG  >  Exported/1200991‑1201127

cGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAg                                                                    <  1:4697721/71‑1 (MQ=255)

  tttATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAgg                                                                   >  1:3736191/1‑70 (MQ=255)

  tttATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAgg                                                                   >  1:3126734/1‑70 (MQ=255)

    tATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTaa                                                                >  1:5435198/1‑71 (MQ=255)

    tATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTa                                                                 <  1:2150604/70‑1 (MQ=255)

    tATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTa                                                                 <  1:3053035/70‑1 (MQ=255)

        gTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                             <  1:912467/70‑1 (MQ=255)

         ttCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAc                                                           <  1:481076/71‑1 (MQ=255)

           cACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCt                                                         >  1:1876963/1‑71 (MQ=255)

            aCTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAcc                                                          <  1:1358070/69‑1 (MQ=255)

            aCTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCt                                                         <  1:3041287/70‑1 (MQ=255)

            aCTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCt                                                         <  1:106621/70‑1 (MQ=255)

            aCTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTg                                                        >  1:4793423/1‑71 (MQ=255)

              tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                       >  1:5539730/1‑70 (MQ=255)

              tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                       >  1:5175328/1‑70 (MQ=255)

              tGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGc                                                       >  1:2059994/1‑70 (MQ=255)

               gTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                             <  1:2673851/63‑1 (MQ=255)

               gTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAc                                                           >  1:2793122/1‑65 (MQ=255)

                tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAg                                                                    <  1:1518438/55‑1 (MQ=255)

                tCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                                                    >  1:1145933/1‑71 (MQ=255)

                    tGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTg                                                >  1:2724003/1‑71 (MQ=255)

                     gACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTg                                                >  1:2265245/1‑70 (MQ=255)

                      aCGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCAACCGTg                                                <  1:2093796/69‑1 (MQ=255)

                         ggTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGatat                                           >  1:5640380/1‑71 (MQ=255)

                          gTAAAGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATc                                          <  1:3545152/71‑1 (MQ=255)

                           tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTaa                                                                >  1:1116551/1‑48 (MQ=255)

                           tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTaa                                                                >  1:2002513/1‑48 (MQ=255)

                           tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTaa                                                                >  1:1601581/1‑48 (MQ=255)

                           tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACg                                                              <  1:4425788/50‑1 (MQ=255)

                           tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACg                                                              <  1:2645510/50‑1 (MQ=255)

                           tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACg                                                              <  1:568434/50‑1 (MQ=255)

                           tAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACg                                                              <  1:2963199/50‑1 (MQ=255)

                             aaGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGa                                        >  1:4609480/1‑70 (MQ=255)

                             aaGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGa                                        >  1:797924/1‑70 (MQ=255)

                             aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                        <  1:3802516/70‑1 (MQ=255)

                             aaGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGa                                        <  1:5313696/70‑1 (MQ=255)

                              aGGCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGatt                                      <  1:1721752/71‑3 (MQ=255)

                               ggCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                     <  1:822517/71‑4 (MQ=255)

                               ggCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                     <  1:3683581/71‑4 (MQ=255)

                               ggCCACCACACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGattc                                     <  1:2724485/71‑4 (MQ=255)

                               ggCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                  >  1:3985719/1‑58 (MQ=255)

                                gCCACCACACCGATCTCGCCATTATTATGGGTCTTGCAGGt                                                                  <  1:5056792/41‑1 (MQ=255)

                                gCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGa                                              >  1:1748441/1‑61 (MQ=255)

                                 ccaccaCACTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGatt                                      <  1:5359276/68‑3 (MQ=255)

                                   accacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGa                                                             <  1:2920708/43‑1 (MQ=255)

                                    ccacACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAcc                                                          <  1:4402897/45‑1 (MQ=255)

                                     cacaCTGATATCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATc                                          <  1:4232881/60‑1 (MQ=255)

                                     cacaCCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAc                                                           >  1:1883555/1‑43 (MQ=255)

                                     cacaCCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAAc                                                           >  1:4101675/1‑43 (MQ=255)

                                      acacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGa                                              >  1:1579627/1‑55 (MQ=255)

                                      acacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTccc                              >  1:2749234/1‑71 (MQ=255)

                                       cacCGATATCGCCATTATTATGGGTCTTGCAGGTaa                                                                >  1:523184/1‑36 (MQ=255)

                                       cacCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCg                             >  1:1683737/1‑71 (MQ=255)

                                        acCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCgg                            >  1:5515268/1‑71 (MQ=255)

                                        acCGATATCGCCATTATTATGGGACTTCCACGTaa                                                                >  1:4293903/1‑35 (MQ=21)

                                         ccGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGt                           >  1:1125878/1‑71 (MQ=255)

                                         ccGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGt                           >  1:3155375/1‑71 (MQ=255)

                                          tgATATCGCCATTATTATGGGCCTTGCGGGTAACCTGCCGGATACCGTGGATATCGat                                       <  1:2910886/57‑2 (MQ=255)

                                            atatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGAc                                       <  1:5570109/56‑1 (MQ=255)

                                            atatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGAc                                       <  1:2376697/56‑1 (MQ=255)

                                             tatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTa                       <  1:2652223/71‑1 (MQ=255)

                                             tatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTa                       <  1:14276/71‑1 (MQ=255)

                                             tatCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTa                       <  1:5695205/71‑1 (MQ=255)

                                              atCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCAc                                                    >  1:5268274/1‑41 (MQ=255)

                                              atCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                      >  1:2619947/1‑71 (MQ=255)

                                               tCGCCATTATTATGGGTCTTGCAGGTAAGGAACCTGCCACCGTGGatat                                           >  1:8664/1‑49 (MQ=255)

                                               tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                  >  1:799848/1‑42 (MQ=255)

                                               tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCg                                                  >  1:181865/1‑42 (MQ=255)

                                               tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGatat                                           >  1:3658793/1‑49 (MQ=255)

                                               tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAtt                     >  1:4179570/1‑71 (MQ=255)

                                               tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                      <  1:1733972/70‑1 (MQ=255)

                                               tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                      <  1:5274320/70‑1 (MQ=255)

                                               tCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAt                      <  1:349867/70‑1 (MQ=255)

                                               tCGCCATTATTATGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTAt                      >  1:2231524/1‑70 (MQ=255)

                                                cGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTa                                   >  1:42484/1‑56 (MQ=255)

                                                  ccATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGt                                                 <  1:1404212/40‑1 (MQ=255)

                                                  ccATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGt                                                 <  1:334685/40‑1 (MQ=255)

                                                  ccATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgc                  <  1:438116/71‑1 (MQ=255)

                                                   cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                     <  1:712724/51‑1 (MQ=255)

                                                   cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                     <  1:166450/51‑1 (MQ=255)

                                                   cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                     <  1:3332616/51‑1 (MQ=255)

                                                   cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                     <  1:4998195/51‑1 (MQ=255)

                                                   cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgcg                 <  1:4439293/71‑1 (MQ=255)

                                                   cATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTcgc                  >  1:458109/1‑70 (MQ=255)

                                                    attattATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                     <  1:4008774/50‑1 (MQ=255)

                                                    attattATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAg                                     <  1:5573431/50‑1 (MQ=255)

                                                    attattATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGa                >  1:3070030/1‑71 (MQ=255)

                                                      tattatGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACg              >  1:5206861/1‑71 (MQ=255)

                                                      tattatGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACg              >  1:5009563/1‑71 (MQ=255)

                                                      tattatGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTAt                      <  1:918092/63‑1 (MQ=255)

                                                       attatGGGCCTGGCGGGTAACCTGCCGGATACCGTGGATATCGATTCCATCCCCAGTTTTATTc                    >  1:2963194/1‑64 (MQ=255)

                                                        ttatGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTa            <  1:4607497/71‑1 (MQ=255)

                                                          atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag           <  1:1330065/70‑1 (MQ=255)

                                                          atGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTag           <  1:5295182/70‑1 (MQ=255)

                                                             ggTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTagaaga       <  1:5659484/71‑1 (MQ=255)

                                                               tCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAgc     <  1:5406685/71‑1 (MQ=255)

                                                                   gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTAtt                     >  1:4062546/1‑51 (MQ=255)

                                                                   gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTc                    >  1:1314832/1‑52 (MQ=255)

                                                                   gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTc                    >  1:3193066/1‑52 (MQ=255)

                                                                   gCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGcgcg  <  1:3789326/70‑1 (MQ=255)

CGTTTATGGTTCACTGTCGCTGACGGGTAAAGGCCACCACACCGATATCGCCATTATTATGGGTCTTGCAGGTAACGAACCTGCCACCGTGGATATCGACAGTATTCCCGGTTTTATTCGCGACGTAGAAGAGCGCG  >  Exported/1200991‑1201127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 36 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`