| Predicted mutation | |||||||
|---|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | freq | annotation | gene | description |
| RA | Exported | 218,166 | C→T | 44.0% | Y51Y (TAC→TAT) | yafF → | conserved hypothetical protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | Exported | 218,166 | 0 | C | T | 44.0% | 54.7 / 157.5 | 135 | Y51Y (TAC→TAT) | yafF | conserved hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base C (48/27); new base T (28/31); total (76/59) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.87e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01 | |||||||||||
Only 100 of 136 total aligned reads displayed.
ACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTA > Exported/218098‑218228 | aCGAATGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCt < 1:1550875/71‑1 (MQ=255) aaTGATTAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTAc < 1:530903/66‑1 (MQ=255) tGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCt < 1:5416401/66‑1 (MQ=255) tGATAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCt < 1:4857886/66‑1 (MQ=255) ataaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTc < 1:3974561/68‑1 (MQ=255) ataaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt > 1:3304418/3‑69 (MQ=255) ataaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt > 1:4955145/3‑69 (MQ=255) taaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAg > 1:5249375/2‑71 (MQ=255) aaGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg > 1:3483338/1‑70 (MQ=255) aGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt < 1:4680723/70‑1 (MQ=255) ggTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTcc < 1:3881365/71‑1 (MQ=255) gTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCt < 1:3867706/71‑1 (MQ=255) tATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtt < 1:2451934/71‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAg < 1:1008257/64‑1 (MQ=255) ttCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCtta < 1:1008818/70‑2 (MQ=255) tCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt < 1:592374/60‑1 (MQ=255) tCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg > 1:5050505/1‑71 (MQ=255) cAAGGCATGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:2166309/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCgg > 1:4845463/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCg < 1:1015791/70‑1 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:3382194/1‑61 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:1116609/1‑61 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCttac > 1:5580842/1‑67 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:1789837/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3109827/1‑71 (MQ=255) cAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:1346359/1‑71 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:1717743/1‑60 (MQ=255) aaGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgg > 1:2833266/1‑70 (MQ=255) aGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACg > 1:1944452/1‑64 (MQ=255) gggTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCgg > 1:3965276/1‑71 (MQ=255) gTTAAGACGTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCgggg < 1:5216980/64‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCggg < 1:710175/62‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGc > 1:3009295/1‑70 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCt > 1:3987943/1‑71 (MQ=255) ttAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGt < 1:1603909/49‑1 (MQ=255) ttAAGACGTAAGATGCGAAAAGAAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCggg < 1:3304238/62‑1 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCtt < 1:2727779/70‑1 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:4586445/1‑49 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg > 1:852468/1‑56 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTg > 1:1125942/1‑56 (MQ=255) aaGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCttt > 1:899542/1‑71 (MQ=255) gACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:3774724/1‑71 (MQ=255) aCGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTGGCGGGGAGCGGGCTTTCgt < 1:3962684/71‑1 (MQ=255) aCGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgt < 1:5643780/71‑1 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGc > 1:745403/1‑53 (MQ=255) cGTAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgta < 1:1786494/71‑1 (MQ=255) gTAAGATGCGAAAAGCAGCCATTGATAGAAACTATCTCGCGt > 1:468321/1‑42 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGt > 1:2020530/1‑42 (MQ=255) gTAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaa > 1:4585127/1‑70 (MQ=255) tAAGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCg < 1:664308/52‑1 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtaa < 1:4927959/70‑2 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtag > 1:1978372/1‑70 (MQ=255) tAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACgggg < 1:3460643/55‑1 (MQ=255) aaGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:2716047/1‑65 (MQ=255) aaGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:4112011/1‑65 (MQ=255) aaGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgta > 1:3841719/1‑68 (MQ=255) aGATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCggg > 1:3489218/1‑52 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCa < 1:5604824/40‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCa < 1:1311927/40‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:1664192/43‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:2275215/43‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:4400580/43‑1 (MQ=255) gATGCGAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGTc < 1:5034668/43‑1 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:2913806/1‑40 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCa > 1:5440118/1‑40 (MQ=255) gATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTc > 1:2241696/1‑63 (MQ=255) gATGCGAAAAGCAGCCATCGACAGAAACTACCTGGCGTc > 1:1177256/1‑39 (MQ=255) aTGCGAAAAGCAGCCATGGACAGAAACTATCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCg > 1:5043737/1‑63 (MQ=255) aTGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACg < 1:2024620/48‑1 (MQ=255) aTGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACg < 1:312583/48‑1 (MQ=255) aTGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACg < 1:2830334/48‑1 (MQ=255) gAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt > 1:2245327/1‑37 (MQ=37) gAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt > 1:3422703/1‑37 (MQ=37) gAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt > 1:5230840/1‑37 (MQ=37) gAAAAGCAGCCATGGATAGAAACTATCTCGCGTCAGt > 1:3451041/1‑37 (MQ=37) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCgtagta > 1:654207/1‑64 (MQ=255) gAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATAcccc < 1:22903/71‑1 (MQ=255) aaaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3709748/1‑44 (MQ=255) aaaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:3008033/1‑44 (MQ=255) aaaaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:4200298/1‑44 (MQ=255) aaGCAGCCATGGATAGAAACTATCTCGCGTCAGTCCTTGCggg < 1:4944522/43‑1 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCgg > 1:640366/1‑42 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCg > 1:1994317/1‑48 (MQ=255) aaGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCtt < 1:418260/53‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTGCg < 1:4232697/39‑1 (MQ=255) gcagcCATGGACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTc > 1:454470/1‑53 (MQ=255) ccATGGACAGAAACTATCTGGCGTCAGTCCTTGCgg < 1:1481279/36‑1 (MQ=255) tGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGt < 1:32265/71‑1 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCg > 1:5167082/1‑37 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGa < 1:269346/59‑1 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTACGGGGAg > 1:5442247/1‑35 (MQ=255) gACAGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTCgtaat > 1:4749352/1‑47 (MQ=39) taGAAACTAGCTGACGTAAGTCCTTGCGGGGAGCAGGGTTTCgta < 1:4048999/44‑1 (MQ=255) cAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCttt > 1:4446192/1‑41 (MQ=255) cAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGAct > 1:1316173/1‑59 (MQ=255) aGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATTCCCCGCCTCTcccc > 1:2861394/1‑64 (MQ=255) aGAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCTTTc > 1:5542994/1‑41 (MQ=255) gAAACTACCTGGCGTCAGTCCTTACGGGGAGCGGGCtt < 1:5284256/38‑1 (MQ=255) tACCTGACGTCAGTCCTTGCGGGGAGCAGGCTTTCgtaaa < 1:1559698/40‑3 (MQ=255) aCCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTaa < 1:5487540/41‑1 (MQ=255) | ACGAATGAGAAGGTATTCAAGGCAGGGTTAAGACGTAAGATGCGAAAAGCAGCCATGGACAGAAACTACCTGGCGTCAGTCCTTGCGGGGAGCGGGCTTTCGTAGTAATACCCCGACTCTCCCCGTCCTTA > Exported/218098‑218228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |