Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,201,858 T→G 21.5% V337V (GTT→GTG sdaA → L‑serine deaminase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,201,8580TG21.5% 150.6 / 43.2 79V337V (GTT→GTGsdaAL‑serine deaminase I
Reads supporting (aligned to +/- strand):  ref base T (31/31);  new base G (7/10);  total (38/41)
Fisher's exact test for biased strand distribution p-value = 5.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGG  >  Exported/1201788‑1201926
                                                                      |                                                                    
gTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAgtt                                                                      >  1:3047871/1‑71 (MQ=255)
 ttACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAgttg                                                                     <  1:1793481/71‑1 (MQ=255)
    cTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAgttggt                                                                   <  1:1758321/70‑1 (MQ=255)
       tATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGc                                                                <  1:5313353/70‑1 (MQ=255)
       tATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGc                                                                >  1:4301139/1‑70 (MQ=255)
         tGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCa                                                              <  1:1872239/70‑1 (MQ=255)
          ggCAGCGAGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCa                                                              <  1:5188995/69‑1 (MQ=255)
            cAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAgtt                                                                      >  1:3836782/1‑59 (MQ=255)
              gCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCa                                                              >  1:50090/1‑65 (MQ=255)
              gCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGa                                                        <  1:1280949/71‑1 (MQ=255)
              gCGGGCGCGATTGGTACATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGcc                                                               >  1:380683/1‑64 (MQ=255)
                   cgcgATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGa                                                        <  1:5555514/66‑1 (MQ=255)
                   cgcgATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTgg                                                   <  1:3280033/71‑1 (MQ=255)
                        ttGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGtt                                              >  1:3776762/1‑71 (MQ=255)
                        ttGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGtt                                              >  1:786844/1‑71 (MQ=255)
                         tGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGtt                                              >  1:3453460/1‑70 (MQ=255)
                           gTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGcc                                           <  1:5654169/71‑1 (MQ=255)
                            tGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCt                                          <  1:2979584/71‑1 (MQ=255)
                             gCATTGTATAAAATGAACGCATCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAg                                                      >  1:2370817/1‑58 (MQ=255)
                              cATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGt                                        >  1:419170/1‑71 (MQ=255)
                              cATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGt                                        >  1:3849571/1‑71 (MQ=255)
                              cATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGt                                        >  1:2230101/1‑71 (MQ=255)
                                ttGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGa                                                        <  1:32093/53‑1 (MQ=255)
                                ttGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTggg                                                  <  1:1045454/59‑1 (MQ=255)
                                  gTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAg                                                             >  1:3311333/1‑46 (MQ=255)
                                  gTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGgtg                                                >  1:2109799/1‑59 (MQ=255)
                                  gTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                    >  1:4111029/1‑71 (MQ=255)
                                   tataAGATGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTc                                      <  1:2274963/68‑1 (MQ=9)
                                   tataAGATGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAAt                                   >  1:2170275/1‑71 (MQ=255)
                                   tataAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGa                                                        <  1:5571954/50‑1 (MQ=255)
                                   tataAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGa                                                        <  1:2739620/50‑1 (MQ=255)
                                    ataAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGAGAAGTGGGTGTTGCCTGTTc                                      <  1:1090400/67‑1 (MQ=255)
                                    aaaaaaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTc                                      <  1:1201554/65‑1 (MQ=255)
                                     taAGATGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAgggtg                                                         <  1:766365/47‑3 (MQ=16)
                                      aaaaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAg                                                      <  1:3502844/49‑1 (MQ=255)
                                      aaaaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGgc                                >  1:2639675/1‑71 (MQ=255)
                                       agaTGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGaa                                                       >  1:4343575/3‑47 (MQ=16)
                                       agaTGAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGaa                                                       >  1:2606903/3‑47 (MQ=16)
                                       aaaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGc                                                                <  1:5607852/38‑1 (MQ=255)
                                       aaaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCa                                                              >  1:3233926/1‑40 (MQ=255)
                                       aaaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAg                                                             >  1:2455315/1‑41 (MQ=255)
                                        aaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCa                                                              >  1:875319/1‑39 (MQ=255)
                                        aaTGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGgctg                              >  1:2878333/1‑71 (MQ=255)
                                        aaTCAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGgctg                              <  1:4324716/71‑1 (MQ=255)
                                           gAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTtggcg                                                <  1:398475/50‑3 (MQ=255)
                                           gAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTtggcg                                                <  1:1311146/50‑3 (MQ=255)
                                           gAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTtggcg                                                <  1:3019909/50‑3 (MQ=255)
                                           gAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCaa                                    >  1:885873/1‑62 (MQ=9)
                                           gAACGCGTCGATTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCaa                                    >  1:3672582/1‑62 (MQ=9)
                                                  tctATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCt                    >  1:1281896/1‑71 (MQ=255)
                                                  tcgaTTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCt                    <  1:3856265/68‑1 (MQ=2)
                                                   ctATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATg                                  <  1:5575919/56‑1 (MQ=255)
                                                   ctATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATg                                  <  1:2330361/56‑1 (MQ=255)
                                                    tATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCtt                   >  1:1033113/1‑70 (MQ=255)
                                                     aTTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGtt                                                    >  1:1172022/1‑35 (MQ=16)
                                                     aTTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGgcgtgg                                             <  1:3633851/43‑3 (MQ=16)
                                                     aTTTCTGGTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGc                                            >  1:3698550/1‑44 (MQ=9)
                                                     aTTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTg                  >  1:423164/1‑70 (MQ=255)
                                                      tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTggg                                                  >  1:4249059/1‑37 (MQ=255)
                                                      tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGgtg                                                >  1:491725/1‑39 (MQ=255)
                                                      tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGgtg                                                >  1:2060467/1‑39 (MQ=255)
                                                      tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGgtg                                                >  1:3539557/1‑39 (MQ=255)
                                                      tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTc                                      <  1:2981976/49‑1 (MQ=255)
                                                      tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTg                  <  1:5639978/69‑1 (MQ=255)
                                                      tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTg                  <  1:1414700/69‑1 (MQ=255)
                                                      tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGc                 <  1:589542/70‑1 (MQ=255)
                                                      tttCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGc                 <  1:1377739/70‑1 (MQ=255)
                                                        tctggTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAATGGCGGCGGCTGGTCTGGCAg               <  1:2404421/67‑1 (MQ=2)
                                                        tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGc                                            <  1:4917877/41‑1 (MQ=255)
                                                        tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGc                                            <  1:5364911/41‑1 (MQ=255)
                                                        tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg               <  1:5555480/70‑1 (MQ=255)
                                                        tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg               >  1:871001/1‑70 (MQ=255)
                                                        tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg               >  1:3259618/1‑70 (MQ=255)
                                                        tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg               <  1:2082192/70‑1 (MQ=255)
                                                        tCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg               >  1:1969824/1‑70 (MQ=255)
                                                          tggTGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTc                                      <  1:2748762/44‑1 (MQ=9)
                                                            gTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAActg          >  1:4693927/1‑71 (MQ=255)
                                                             tGCTGAAGTGGGTTGCCAGGGTGAAGTTGGCGTGGCGTGCTCAAt                                   <  1:2913148/45‑1 (MQ=255)
                                                               cGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAg               <  1:4713454/63‑1 (MQ=255)
                                                                 gAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGaa             >  1:1592768/1‑63 (MQ=255)
                                                                  aaGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGaa             >  1:5674933/1‑62 (MQ=255)
                                                                  aaGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGc    <  1:3233971/71‑1 (MQ=255)
                                                                   aGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCg   <  1:5418114/71‑1 (MQ=255)
                                                                    gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                    <  1:2824695/37‑1 (MQ=255)
                                                                    gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                    <  1:3918597/37‑1 (MQ=255)
                                                                    gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                    <  1:4013511/37‑1 (MQ=255)
                                                                    gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCaa                                    <  1:4128865/37‑1 (MQ=255)
                                                                    gttggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCgg  <  1:1424339/71‑1 (MQ=255)
                                                                      tggttgCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCTGGTCTTGCAGAACTGCTGGGCgg  >  1:4521925/1‑69 (MQ=255)
                                                                      |                                                                    
GTTACTTTATGGCAGCGGGCGCGATTGGTGCATTGTATAAAATGAACGCCTCTATTTCCGGTGCGGAAGTTGGTTGCCAGGGCGAAGTGGGTGTTGCCTGTTCAATGGCTGCTGCGGGTCTTGCAGAACTGCTGGGCGG  >  Exported/1201788‑1201926

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: