Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 106,058 G→T 13.3% S78S (TCC→TCA aroP ← aromatic amino acid transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported106,0580GT13.3% 81.6 / 2.4 30S78S (TCC→TCAaroParomatic amino acid transporter
Reads supporting (aligned to +/- strand):  ref base G (13/13);  new base T (2/2);  total (15/15)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.12e-01

GTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCA  >  Exported/105988‑106127
                                                                      |                                                                     
gTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAg                                                                       <  1:2718070/71‑1 (MQ=255)
     cAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGcc                                                                  >  1:2739150/1‑71 (MQ=255)
           ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc                                                            <  1:361639/71‑1 (MQ=255)
           ttCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGAc                                                            <  1:3509339/71‑1 (MQ=255)
             ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg                                                          >  1:994333/1‑71 (MQ=255)
             ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg                                                          >  1:4309736/1‑71 (MQ=255)
             ccagccagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAg                                                          >  1:1694569/1‑71 (MQ=255)
                  cagAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttct                                                     >  1:1899357/1‑71 (MQ=255)
                       gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac                                                >  1:2519690/1‑71 (MQ=255)
                       gCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGac                                                >  1:4109909/1‑71 (MQ=255)
                                  aaaaCTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGaccac                                             >  1:5197546/1‑63 (MQ=255)
                                      cTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAg                                 <  1:5270303/71‑1 (MQ=255)
                                        tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt                               <  1:1085254/70‑1 (MQ=255)
                                        tccccAGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCt                               <  1:5349701/70‑1 (MQ=255)
                                        gCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCt                               >  1:2700314/1‑71 (MQ=255)
                                             aGTATTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCg                           >  1:1605763/1‑70 (MQ=255)
                                             aGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGc                          <  1:606703/71‑1 (MQ=255)
                                              gTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCa                                      >  1:805067/1‑58 (MQ=255)
                                               tATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGttcttc                                                   <  1:2509002/44‑1 (MQ=255)
                                                aTTTATAGGCAAAGTGGGCAAATGAACCGGATACCGGCTCCTCAACCACCATTTCGCCAAGCTGGCGCAt                        >  1:1419409/1‑70 (MQ=255)
                                                 tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTg                              <  1:856727/63‑1 (MQ=255)
                                                 tttATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATg                       <  1:5494395/70‑1 (MQ=255)
                                                          aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCt                               >  1:3789774/1‑53 (MQ=255)
                                                          aaaGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAgg             >  1:4151564/1‑71 (MQ=255)
                                                             gTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTTACCCa                                  <  1:2182475/47‑1 (MQ=255)
                                                              tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCAtt                                         <  1:4094996/39‑1 (MQ=255)
                                                              tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCAtt                                         <  1:3799355/39‑1 (MQ=255)
                                                              tGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAt         <  1:178533/71‑1 (MQ=255)
                                                              tGGCTAAAGGAGCATGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGAt         <  1:482131/71‑1 (MQ=255)
                                                                    aaGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAAc    >  1:3476152/1‑70 (MQ=255)
                                                                     aGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCa  <  1:2706303/71‑1 (MQ=255)
                                                                      |                                                                     
GTACCCAGTAGTTCCAGCCAGAGGCGAAACCGGCAAAACTGCCCCAGTATTTATAAGCAAAGTGGCTAAAGGAGCCTGCGACAGGTTCTTCGACCACCATTTCACCCAGCTGACGCATGATCAGAAAGGCGATAAAACCA  >  Exported/105988‑106127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: