Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,211,220 C→A 12.4% L44L (CTG→CTT cspC ← stress protein, member of the CspA‑family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,211,2200CA12.4% 389.3 / 25.4 128L44L (CTG→CTTcspCstress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base C (64/48);  new base A (2/14);  total (66/62)
Fisher's exact test for biased strand distribution p-value = 9.24e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 135 total aligned reads displayed.

GCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCG  >  Exported/1211149‑1211287
                                                                       |                                                                   
gCTGTTACGTTAACAGCTGCCGGACCTT‑‑TGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGcc                                                                     <  1:1452106/70‑1 (MQ=255)
 cTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGcc                                                                     <  1:440291/71‑1 (MQ=255)
 cTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGcc                                                                     <  1:2481588/71‑1 (MQ=255)
 cTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGcc                                                                     <  1:2367881/71‑1 (MQ=255)
    ttACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCag                                                                   <  1:5478803/70‑1 (MQ=255)
     tACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCagag                                                                 >  1:1305664/1‑71 (MQ=255)
      aCGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCagag                                                                 >  1:1680577/1‑70 (MQ=255)
        gTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGttt                                                              <  1:1766179/71‑1 (MQ=255)
         ttAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGtttt                                                             <  1:567544/71‑1 (MQ=255)
         ttAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGttt                                                              <  1:3519405/70‑1 (MQ=255)
          tAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGtttt                                                             >  1:276835/1‑70 (MQ=255)
           aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg                                                            <  1:100053/70‑1 (MQ=255)
              aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                        <  1:5466415/71‑1 (MQ=255)
               gCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                        >  1:2169202/1‑70 (MQ=255)
                   ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa                                                   >  1:3423870/1‑71 (MQ=255)
                   ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa                                                   <  1:1369501/71‑1 (MQ=255)
                    cGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGcc                                                       <  1:3180194/66‑1 (MQ=255)
                        ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGttt                                                              >  1:329064/1‑55 (MQ=255)
                        ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                              <  1:773513/71‑1 (MQ=255)
                        ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                              <  1:575558/71‑1 (MQ=255)
                         cTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                  >  1:46080/1‑66 (MQ=255)
                           ttCTGGACGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                            >  1:1498086/1‑70 (MQ=255)
                            tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                          >  1:935144/1‑55 (MQ=255)
                            tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                            <  1:2718993/69‑1 (MQ=255)
                            tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                            >  1:2660368/1‑69 (MQ=255)
                             cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                          >  1:3414663/1‑54 (MQ=255)
                             cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa                                                      >  1:2554025/1‑58 (MQ=255)
                                gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCATCCAGAGTTTTGAAGCCATTACCCTGGATAGc                                        <  1:4264330/69‑1 (MQ=255)
                                gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAg                                         <  1:5100298/68‑1 (MQ=255)
                                gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg                                       <  1:1299178/70‑1 (MQ=255)
                                  cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                    <  1:3148808/71‑1 (MQ=255)
                                  cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                    <  1:1933932/71‑1 (MQ=255)
                                   gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                            >  1:63875/1‑62 (MQ=255)
                                   gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATAACCCTGGa                                            >  1:321761/1‑62 (MQ=255)
                                    tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGtttt                                                             <  1:1854986/44‑1 (MQ=255)
                                    tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                        <  1:181383/49‑1 (MQ=255)
                                    tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                    >  1:854272/1‑69 (MQ=255)
                                    tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga                                     >  1:1729316/1‑68 (MQ=255)
                                     ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAg                                 >  1:2486325/1‑71 (MQ=255)
                                      cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                          >  1:2168864/1‑45 (MQ=255)
                                      cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                          >  1:2539915/1‑45 (MQ=255)
                                      cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                          >  1:5601381/1‑45 (MQ=255)
                                      cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                          >  1:3838492/1‑45 (MQ=255)
                                      cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGa                                                           >  1:2520168/1‑44 (MQ=255)
                                      cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                              <  1:1263722/57‑1 (MQ=255)
                                      cTGAATGTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgt                                >  1:739256/1‑71 (MQ=255)
                                       tGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGcc                                                       <  1:3249401/47‑1 (MQ=255)
                                         aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                               <  1:5304883/69‑1 (MQ=255)
                                         aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                             <  1:5534542/71‑1 (MQ=255)
                                          aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc                            >  1:1514981/1‑71 (MQ=255)
                                          aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTCAAAACCATTAGTCTGGATTGCAGAGAAgtg                               <  1:1848828/68‑1 (MQ=255)
                                          aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                             <  1:5208823/70‑1 (MQ=255)
                                           tttCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCAt                                                     <  1:2096822/45‑1 (MQ=255)
                                           tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                  <  1:4815252/48‑1 (MQ=255)
                                           tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                  <  1:3847426/48‑1 (MQ=255)
                                           tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                           <  1:2215967/71‑1 (MQ=255)
                                           tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                           <  1:2343692/71‑1 (MQ=255)
                                           tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                           <  1:367860/71‑1 (MQ=255)
                                           tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                           <  1:394843/71‑1 (MQ=255)
                                             tCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGa                          <  1:2607437/70‑1 (MQ=255)
                                             tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGcc                                                       >  1:1758978/1‑41 (MQ=255)
                                             tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                 >  1:2481993/1‑47 (MQ=255)
                                                aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                               <  1:4910506/62‑1 (MQ=255)
                                                aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca                       >  1:2992507/1‑70 (MQ=255)
                                                aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca                       >  1:202511/1‑70 (MQ=255)
                                                 aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                 >  1:5322828/1‑43 (MQ=255)
                                                  cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                        <  1:5246201/35‑1 (MQ=255)
                                                     aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt                 >  1:4738548/1‑71 (MQ=255)
                                                       ctttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAgt                                >  1:3540719/3‑54 (MQ=255)
                                                       cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                 >  1:5339600/1‑37 (MQ=255)
                                                       cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                              <  1:3418119/40‑1 (MQ=255)
                                                        gcgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCttt                <  1:59939/66‑1 (MQ=255)
                                                        gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                 >  1:2607791/1‑36 (MQ=255)
                                                         ttCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgct             >  1:1588597/1‑71 (MQ=255)
                                                         cgcTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTtgc              <  1:5022279/68‑1 (MQ=255)
                                                          tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg            >  1:4519688/1‑71 (MQ=255)
                                                          tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg            >  1:5603969/1‑71 (MQ=255)
                                                           cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc           >  1:1254573/1‑71 (MQ=255)
                                                            tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGAt                                           <  1:5508843/38‑1 (MQ=255)
                                                            tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc           >  1:468109/1‑70 (MQ=255)
                                                             gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa         >  1:5551196/1‑71 (MQ=255)
                                                             gACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGa                                            >  1:2001823/1‑36 (MQ=16)
                                                                 ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                             <  1:4446477/47‑1 (MQ=255)
                                                                 ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                             <  1:3684160/47‑1 (MQ=255)
                                                                 ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                             <  1:5523298/47‑1 (MQ=255)
                                                                 ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                             <  1:138043/47‑1 (MQ=255)
                                                                  tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc    >  1:3933927/1‑71 (MQ=255)
                                                                  tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc    >  1:2276753/1‑71 (MQ=255)
                                                                  tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                 <  1:1788695/58‑1 (MQ=255)
                                                                  tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                 <  1:5328213/58‑1 (MQ=255)
                                                                  tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                 <  1:3821948/58‑1 (MQ=255)
                                                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgaga                                   >  1:1257439/1‑39 (MQ=255)
                                                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                             >  1:574708/1‑45 (MQ=255)
                                                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg     <  1:3489174/69‑1 (MQ=255)
                                                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg     <  1:1528557/69‑1 (MQ=255)
                                                                   cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc    <  1:1600824/70‑1 (MQ=255)
                                                                    aGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCg  <  1:2632015/71‑1 (MQ=255)
                                                                      ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                    <  1:3270136/35‑1 (MQ=255)
                                                                      ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                    <  1:2987294/35‑1 (MQ=255)
                                                                       cAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCt                  >  1:5316754/1‑52 (MQ=255)
                                                                       |                                                                   
GCTGTTACGTTAACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCG  >  Exported/1211149‑1211287

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: