Predicted mutation
evidence seq id position mutation freq annotation gene description
RA Exported 1,211,229 G→A 15.3% F41F (TTC→TTT cspC ← stress protein, member of the CspA‑family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*Exported1,211,2290GA15.3% 335.6 / 41.0 137F41F (TTC→TTTcspCstress protein, member of the CspA‑family
Reads supporting (aligned to +/- strand):  ref base G (64/52);  new base A (3/18);  total (67/70)
Fisher's exact test for biased strand distribution p-value = 6.36e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

AACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAG  >  Exported/1211160‑1211298
                                                                     |                                                                     
aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg                                                                       <  1:100053/70‑1 (MQ=255)
aaCAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg                                                                       <  1:2518423/70‑1 (MQ=255)
   aGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                                   <  1:2106075/71‑1 (MQ=255)
    gCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                                   >  1:2169202/1‑70 (MQ=255)
      tGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGa                                                                      >  1:785200/1‑65 (MQ=255)
       gCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt                                                                >  1:4532151/1‑70 (MQ=255)
        ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa                                                              <  1:1369501/71‑1 (MQ=255)
        ccGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa                                                              >  1:3423870/1‑71 (MQ=255)
             ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                         <  1:773513/71‑1 (MQ=255)
             ccTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                         <  1:575558/71‑1 (MQ=255)
              cTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                             >  1:98333/1‑66 (MQ=255)
                 tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                                     >  1:935144/1‑55 (MQ=255)
                 tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                       >  1:626907/1‑69 (MQ=255)
                 tCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                       >  1:2660368/1‑69 (MQ=255)
                  cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                                     >  1:3414663/1‑54 (MQ=255)
                  cTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCa                                                                 >  1:2554025/1‑58 (MQ=255)
                     gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCATCCAGAGTTTTGAAGCCATTACCCTGGATAGc                                                   <  1:4264330/69‑1 (MQ=255)
                     gCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCg                                                  <  1:1299178/70‑1 (MQ=255)
                       cGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                               <  1:3148808/71‑1 (MQ=255)
                        gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGa                                                       >  1:63875/1‑62 (MQ=255)
                        gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                               >  1:3579978/1‑70 (MQ=255)
                        gTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATAACCCTGGa                                                       >  1:321761/1‑62 (MQ=255)
                         tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg                                                                       <  1:1983637/45‑1 (MQ=255)
                         tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                                   <  1:181383/49‑1 (MQ=255)
                         tCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGga                                                >  1:1729316/1‑68 (MQ=255)
                          ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTg                                                                       <  1:3484238/44‑1 (MQ=255)
                          ccTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAg                                            >  1:2486325/1‑71 (MQ=255)
                           cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                                     >  1:2539915/1‑45 (MQ=255)
                           cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                                     >  1:2750985/1‑45 (MQ=255)
                           cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                                     >  1:5085462/1‑45 (MQ=255)
                           cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                                     >  1:5601381/1‑45 (MQ=255)
                           cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                                     >  1:1543151/1‑45 (MQ=255)
                           cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGaa                                                                     >  1:2168864/1‑45 (MQ=255)
                           cTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTg                                                         <  1:1263722/57‑1 (MQ=255)
                            gtgaTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAgtg                                          <  1:3133154/68‑1 (MQ=255)
                              aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTa                                                              >  1:4811216/1‑49 (MQ=255)
                              aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                        <  1:5534542/71‑1 (MQ=255)
                              aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                        <  1:1780813/71‑1 (MQ=255)
                              aaTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                        >  1:5393203/1‑71 (MQ=255)
                               aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTAc                                       >  1:1514981/1‑71 (MQ=255)
                               aTTTCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTCAAAACCATTAGTCTGGATTGCAGAGAAgtg                                          <  1:1848828/68‑1 (MQ=255)
                               aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                        <  1:5208823/70‑1 (MQ=255)
                               aTTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTAc                                       >  1:3218852/1‑71 (MQ=255)
                                tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAc                                                             <  1:3847426/48‑1 (MQ=255)
                                tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                      <  1:367860/71‑1 (MQ=255)
                                tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                      <  1:2215967/71‑1 (MQ=255)
                                tttCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACg                                      <  1:2343692/71‑1 (MQ=255)
                                  tCGAACTCTACGCGCTGACCTTCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGa                                     <  1:2607437/70‑1 (MQ=255)
                                  tCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                            >  1:2481993/1‑47 (MQ=255)
                                     aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                          <  1:4910506/62‑1 (MQ=255)
                                     aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAgtg                                          <  1:2621380/62‑1 (MQ=255)
                                     aaCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAaca                                  >  1:202511/1‑70 (MQ=255)
                                      aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                            >  1:3794890/1‑43 (MQ=255)
                                      aCTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                            >  1:5322828/1‑43 (MQ=255)
                                       cTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGc                                                                   <  1:5246201/35‑1 (MQ=255)
                                          aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCAt                                                                >  1:530325/1‑35 (MQ=255)
                                          aaCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCtt                            >  1:4738548/1‑71 (MQ=255)
                                           aCGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                               <  1:4306100/51‑1 (MQ=255)
                                            ctttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAGAGAAgt                                           >  1:3540719/3‑54 (MQ=255)
                                            cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                            >  1:5516915/1‑37 (MQ=255)
                                            cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                            >  1:1834766/1‑37 (MQ=255)
                                            cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                            >  1:148853/1‑37 (MQ=255)
                                            cGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtg                          <  1:1692410/71‑1 (MQ=255)
                                             tttCTGACCTTCGTCCAGAGATTTGTAACCATCGTTCTGGATAGCAgaga                                              <  1:3311892/49‑1 (MQ=255)
                                             gTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTAcc                                                            >  1:2607791/1‑36 (MQ=255)
                                               tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg                       >  1:5603969/1‑71 (MQ=255)
                                               tCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctg                       >  1:4519688/1‑71 (MQ=255)
                                                cTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc                      >  1:1254573/1‑71 (MQ=255)
                                                 tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGAt                                                      <  1:5508843/38‑1 (MQ=255)
                                                 tGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTtgctgc                      >  1:468109/1‑70 (MQ=255)
                                                  gACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCa                    >  1:5551196/1‑71 (MQ=255)
                                                      ttCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTa                                        <  1:138043/47‑1 (MQ=255)
                                                       tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc               >  1:3933927/1‑71 (MQ=255)
                                                       tCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc               >  1:2276753/1‑71 (MQ=255)
                                                       tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                            <  1:3821948/58‑1 (MQ=255)
                                                       tCAGCAAGAGTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCtt                            <  1:5328213/58‑1 (MQ=255)
                                                        cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGgaga                                              >  1:1257439/1‑39 (MQ=255)
                                                        cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTa                                        >  1:574708/1‑45 (MQ=255)
                                                        cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAg                <  1:1528557/69‑1 (MQ=255)
                                                        cAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGc               <  1:1600824/70‑1 (MQ=255)
                                                           ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                               <  1:2987294/35‑1 (MQ=255)
                                                           ccAGAGTTTTGAAGCCATTACCCTGGATAGCGgag                                               <  1:3270136/35‑1 (MQ=255)
                                                            cAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCt                             >  1:5316754/1‑52 (MQ=255)
                                                            aagagTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAg          >  1:2641007/2‑71 (MQ=255)
                                                             agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGact         <  1:2794389/71‑3 (MQ=255)
                                                             agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAg          >  1:5312850/1‑70 (MQ=255)
                                                             agagTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGt         <  1:1759384/71‑1 (MQ=255)
                                                               agTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTaa       <  1:5371059/71‑1 (MQ=255)
                                                               agTTTTAAATCCATTAGTCTGGATTGCAGAGAAGTGTACGAac                                   <  1:2637403/43‑1 (MQ=16)
                                                               agTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTaa       <  1:4061192/71‑1 (MQ=16)
                                                                gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt      <  1:2078635/71‑1 (MQ=255)
                                                                gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt      <  1:5075619/71‑1 (MQ=255)
                                                                gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt      <  1:5394219/71‑1 (MQ=255)
                                                                gTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAAt      >  1:2661813/1‑71 (MQ=255)
                                                                gTTTTAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGCCGTCTTCCGGAGTAAt      <  1:1547175/71‑1 (MQ=16)
                                                                 ttttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATg     <  1:4816761/71‑1 (MQ=255)
                                                                  tttAAAACCATTAGTCTGGATTGCAGAGAAGTGTACGAACACGTCTTTGCTGccg                    <  1:5545042/55‑2 (MQ=16)
                                                                   ttGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGa    <  1:2205268/70‑1 (MQ=255)
                                                                    tGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAg  >  1:1975074/1‑71 (MQ=255)
                                                                     gAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATc                              >  1:661413/1‑42 (MQ=255)
                                                                     |                                                                     
AACAGCTGCCGGACCTTTCTGGCCGTCCTGAATTTCGAACTCAACGTTCTGACCTTCAGCCAGAGTTTTGAAGCCATTACCCTGGATAGCGGAGAAGTGTACGAACACATCTTTGCTGCCATCAGCCGGAGTAATGAAG  >  Exported/1211160‑1211298

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: